PMID- 37628604
OWN - NLM
STAT- MEDLINE
DCOM- 20230828
LR  - 20230829
IS  - 2073-4425 (Electronic)
IS  - 2073-4425 (Linking)
VI  - 14
IP  - 8
DP  - 2023 Jul 28
TI  - Arabidopsis RAD16 Homologues Are Involved in UV Tolerance and Growth.
LID - 10.3390/genes14081552 [doi]
LID - 1552
AB  - In plants, prolonged exposure to ultraviolet (UV) radiation causes harmful DNA 
      lesions. Nucleotide excision repair (NER) is an important DNA repair mechanism 
      that operates via two pathways: transcription coupled repair (TC-NER) and global 
      genomic repair (GG-NER). In plants and mammals, TC-NER is initiated by the 
      Cockayne Syndrome A and B (CSA/CSB) complex, whereas GG-NER is initiated by the 
      Damaged DNA Binding protein 1/2 (DDB1/2) complex. In the yeast Saccharomyces 
      cerevisiae (S. cerevisiae), GG-NER is initiated by the Radiation Sensitive 7 and 
      16, (RAD7/16) complex. Arabidopsis thaliana has two homologues of yeast RAD16, 
      At1g05120 and At1g02670, which we named AtRAD16 and AtRAD16b, respectively. In 
      this study, we characterized the roles of AtRAD16 and AtRAD16b. Arabidopsis rad16 
      and rad16b null mutants exhibited increased UV sensitivity. Moreover, AtRAD16 
      overexpression increased plant UV tolerance. Thus, AtRAD16 and AtRAD16b 
      contribute to plant UV tolerance and growth. Additionally, we found physical 
      interaction between AtRAD16 and AtRAD7. Thus, the Arabidopsis RAD7/16 complex is 
      functional in plant NER. Furthermore, AtRAD16 makes a significant contribution to 
      Arabidopsis UV tolerance compared to the DDB1/2 and the CSB pathways. This is the 
      first time the role and interaction of DDB1/2, RAD7/16, and CSA/CSB components in 
      a single system have been studied.
FAU - Alrayes, Linda
AU  - Alrayes L
AD  - Department of Biological Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, 
      Canada.
FAU - Stout, Jake
AU  - Stout J
AUID- ORCID: 0000-0002-0193-9066
AD  - Department of Biological Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, 
      Canada.
FAU - Schroeder, Dana
AU  - Schroeder D
AD  - Department of Biological Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, 
      Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230728
PL  - Switzerland
TA  - Genes (Basel)
JT  - Genes
JID - 101551097
RN  - 0 (RAD7 protein, S cerevisiae)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 3.6.1.3 (RAD16 protein, S cerevisiae)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
SB  - IM
MH  - Animals
MH  - Saccharomyces cerevisiae
MH  - *Arabidopsis/genetics
MH  - *Cockayne Syndrome
MH  - DNA Repair/genetics
MH  - Ultraviolet Rays/adverse effects
MH  - Mammals
MH  - DNA-Binding Proteins/genetics
MH  - *Saccharomyces cerevisiae Proteins
MH  - Adenosine Triphosphatases
PMC - PMC10454142
OTO - NOTNLM
OT  - Arabidopsis
OT  - CSA/CSB
OT  - DDB1/2
OT  - Nucleotide excision repair
OT  - RAD7/16
OT  - UV sensitivity
COIS- The authors declare no conflict of interest.
EDAT- 2023/08/26 10:45
MHDA- 2023/08/28 07:16
PMCR- 2023/07/28
CRDT- 2023/08/26 01:13
PHST- 2023/06/30 00:00 [received]
PHST- 2023/07/21 00:00 [revised]
PHST- 2023/07/24 00:00 [accepted]
PHST- 2023/08/28 07:16 [medline]
PHST- 2023/08/26 10:45 [pubmed]
PHST- 2023/08/26 01:13 [entrez]
PHST- 2023/07/28 00:00 [pmc-release]
AID - genes14081552 [pii]
AID - genes-14-01552 [pii]
AID - 10.3390/genes14081552 [doi]
PST - epublish
SO  - Genes (Basel). 2023 Jul 28;14(8):1552. doi: 10.3390/genes14081552.