PMID- 37630679
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230829
IS  - 2076-2607 (Print)
IS  - 2076-2607 (Electronic)
IS  - 2076-2607 (Linking)
VI  - 11
IP  - 8
DP  - 2023 Aug 20
TI  - Native Pig Neutrophil Products: Insights into Their Antimicrobial Activity.
LID - 10.3390/microorganisms11082119 [doi]
LID - 2119
AB  - Cationic antimicrobial peptides are molecules with potential applications for 
      treating infections due to their antimicrobial and immunomodulatory properties. 
      The aim of this work was to explore the antimicrobial activity and mechanisms of 
      action of a porcine neutrophil cathelicidin mixture (MPPN). Gram-positive and 
      Gram-negative bacteria were used to determine the minimum inhibitory 
      concentration (MIC) and experiments of both time-kill kinetics and effects on 
      growth curves were performed. Planar black lipid bilayer conductance was measured 
      to analyze the interaction of MPPN with lipid bilayers. Visualization of 
      bacterial surfaces and membrane alterations was achieved using atomic force 
      microscopy and transmission electron microscopy. The effects on the activity of 
      efflux pumps (EPs) were studied with an intracellular accumulation of acridine 
      orange (AO) assay. In E. coli, MPPN behaves as a bactericide at high 
      concentrations and as a bacteriostatic at lower concentrations. The 
      bacteriostatic effect was also observed for slightly shorter periods in S. 
      enterica. The mixture was not active on S. aureus. The increase in AO 
      accumulation in the presence of MPPN indicates that, at least in E. coli, the 
      mixture causes inhibition of the EP function. Observed and detected variable 
      conductance events demonstrate a strong MPPN effect on lipid bilayers. Damage to 
      the structure of treated E. coli indicates that MPPN induces alterations in the 
      bacterial surface. The use of AMPs capable of inhibiting EP can be seen as a good 
      tool to combat antimicrobial resistance since they could be used alone or in 
      combination with other conventional antibiotics to which bacteria have become 
      resistant.
FAU - Fernandez-De La Cruz, Eric
AU  - Fernandez-De La Cruz E
AD  - Laboratory of Molecular Microbiology & Antimicrobials, Department of Pathology & 
      Experimental Therapeutics, Faculty of Medicine & Health Sciences, 
      IDIBELL-University of Barcelona, Campus Bellvitge, 08907 L'Hospitalet de 
      Llobregat, Spain.
FAU - Wessely-Szponder, Joanna
AU  - Wessely-Szponder J
AUID- ORCID: 0000-0002-8815-0630
AD  - Sub-Department of Pathophysiology, Department of Preclinical Veterinary Sciences, 
      Faculty of Veterinary Medicine, University of Life Sciences, 20-950 Lublin, 
      Poland.
FAU - Vinas, Miguel
AU  - Vinas M
AUID- ORCID: 0000-0003-2073-3386
AD  - Laboratory of Molecular Microbiology & Antimicrobials, Department of Pathology & 
      Experimental Therapeutics, Faculty of Medicine & Health Sciences, 
      IDIBELL-University of Barcelona, Campus Bellvitge, 08907 L'Hospitalet de 
      Llobregat, Spain.
FAU - Vinuesa, Teresa
AU  - Vinuesa T
AUID- ORCID: 0000-0002-3536-4109
AD  - Laboratory of Molecular Microbiology & Antimicrobials, Department of Pathology & 
      Experimental Therapeutics, Faculty of Medicine & Health Sciences, 
      IDIBELL-University of Barcelona, Campus Bellvitge, 08907 L'Hospitalet de 
      Llobregat, Spain.
FAU - Merlos, Alexandra
AU  - Merlos A
AD  - Laboratory of Molecular Microbiology & Antimicrobials, Department of Pathology & 
      Experimental Therapeutics, Faculty of Medicine & Health Sciences, 
      IDIBELL-University of Barcelona, Campus Bellvitge, 08907 L'Hospitalet de 
      Llobregat, Spain.
FAU - Jorba, Marta
AU  - Jorba M
AUID- ORCID: 0000-0002-8492-6331
AD  - Laboratory of Molecular Microbiology & Antimicrobials, Department of Pathology & 
      Experimental Therapeutics, Faculty of Medicine & Health Sciences, 
      IDIBELL-University of Barcelona, Campus Bellvitge, 08907 L'Hospitalet de 
      Llobregat, Spain.
FAU - Espinal, Paula
AU  - Espinal P
AUID- ORCID: 0000-0002-1014-8936
AD  - Laboratory of Molecular Microbiology & Antimicrobials, Department of Pathology & 
      Experimental Therapeutics, Faculty of Medicine & Health Sciences, 
      IDIBELL-University of Barcelona, Campus Bellvitge, 08907 L'Hospitalet de 
      Llobregat, Spain.
FAU - Fuste, Ester
AU  - Fuste E
AUID- ORCID: 0000-0002-3561-9765
AD  - Laboratory of Molecular Microbiology & Antimicrobials, Department of Pathology & 
      Experimental Therapeutics, Faculty of Medicine & Health Sciences, 
      IDIBELL-University of Barcelona, Campus Bellvitge, 08907 L'Hospitalet de 
      Llobregat, Spain.
AD  - Department of Public Health, Mental Health and Maternal and Child Nursing, 
      University of Barcelona, Campus Bellvitge, 08907 L'Hospitalet de Llobregat, 
      Spain.
LA  - eng
GR  - BARNAPA 201/C/2018 to M.V./MARATO TV3 Foundation/
PT  - Journal Article
DEP - 20230820
PL  - Switzerland
TA  - Microorganisms
JT  - Microorganisms
JID - 101625893
PMC - PMC10459379
OTO - NOTNLM
OT  - antimicrobial peptides
OT  - atomic force microscopy
OT  - black lipid bilayer
OT  - cathelicidins
OT  - efflux pumps
OT  - membrane disruption
OT  - transmission electron microscopy
COIS- The authors declare no conflict of interest.
EDAT- 2023/08/26 10:45
MHDA- 2023/08/26 10:46
PMCR- 2023/08/20
CRDT- 2023/08/26 01:26
PHST- 2023/07/14 00:00 [received]
PHST- 2023/08/13 00:00 [revised]
PHST- 2023/08/17 00:00 [accepted]
PHST- 2023/08/26 10:46 [medline]
PHST- 2023/08/26 10:45 [pubmed]
PHST- 2023/08/26 01:26 [entrez]
PHST- 2023/08/20 00:00 [pmc-release]
AID - microorganisms11082119 [pii]
AID - microorganisms-11-02119 [pii]
AID - 10.3390/microorganisms11082119 [doi]
PST - epublish
SO  - Microorganisms. 2023 Aug 20;11(8):2119. doi: 10.3390/microorganisms11082119.