PMID- 37631917 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20230829 IS - 2076-393X (Print) IS - 2076-393X (Electronic) IS - 2076-393X (Linking) VI - 11 IP - 8 DP - 2023 Aug 9 TI - Protective Efficacy of a Candidate Live-Attenuated Vaccine Derived from the SD-R Strain against NADC34-like Porcine Reproductive and Respiratory Syndrome Virus. LID - 10.3390/vaccines11081349 [doi] LID - 1349 AB - NADC34-like porcine reproductive and respiratory syndrome virus (PRRSV) strains were first detected in China in 2017 and became major circulating strains in 2021. Our previous study showed that the live-attenuated vaccine candidate SD-R strain could provide broad cross-protection against different NADC30-like PRRSVs (sublineage 1.8). However, the protective effect of SD-R against NADC34-like PRRSV is unclear. Here, a novel NADC34-like PRRSV, LNTZJ1341-2012, was isolated from a pig farm experiencing disease in 2020. Sequence analysis revealed that LNTZJ1341-2012 belonged to PRRSV-2 sublineage 1.5, exhibited the same Nsp2 amino-acid deletion characteristics as IA/2014/NADC34, and had not recombined with other strains. Additionally, a good challenge model was established to evaluate the protection afforded by the candidate SD-R vaccine against infection with a representative NADC34-like strain (LNTZJ1341-2012). The control piglets in the challenge experiment displayed clinical signs typical of PRRSV infection, including transient fever, high viremia, mild clinical symptoms, and histopathological changes in the lungs and submaxillary lymph nodes. In contrast, SD-R vaccination significantly reduced serum and lung tissue viral loads, and vaccinated piglets did not show any clinical symptoms or histopathological changes. Our results demonstrated that LNTZJ1341-2012 is a mildly virulent NADC34-like PRRSV and that the live-attenuated vaccine SD-R can prevent the onset of clinical signs upon challenge with the NADC34-like PRRSV LNTZJ1341-2012 strain, indicating that SD-R is a promising vaccine candidate for the swine industry. FAU - Xu, Hu AU - Xu H AUID- ORCID: 0009-0008-3316-8041 AD - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. FAU - Li, Chao AU - Li C AD - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. FAU - Gong, Bangjun AU - Gong B AD - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. FAU - Li, Wansheng AU - Li W AD - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. FAU - Guo, Zhenyang AU - Guo Z AD - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. FAU - Sun, Qi AU - Sun Q AD - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. FAU - Zhao, Jing AU - Zhao J AD - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. FAU - Xiang, Lirun AU - Xiang L AD - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. FAU - Li, Jinhao AU - Li J AD - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. FAU - Tang, Yan-Dong AU - Tang YD AUID- ORCID: 0000-0002-5690-2690 AD - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. FAU - Leng, Chaoliang AU - Leng C AD - Henan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Nanyang Normal University, Nanyang 473061, China. FAU - Wang, Qian AU - Wang Q AD - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. FAU - Peng, Jinmei AU - Peng J AD - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. FAU - Zhou, Guohui AU - Zhou G AD - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. FAU - Liu, Huairan AU - Liu H AD - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. FAU - An, Tongqing AU - An T AUID- ORCID: 0000-0003-4619-4587 AD - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. FAU - Cai, Xuehui AU - Cai X AD - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. FAU - Tian, Zhi-Jun AU - Tian ZJ AD - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. FAU - Zhang, Hongliang AU - Zhang H AUID- ORCID: 0000-0003-0705-6800 AD - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. LA - eng GR - 32002315/National Natural Science Foundation of China/ GR - 32172890/National Natural Science Foundation of China/ GR - YQ2022C042/Natural Science Foundation of Heilongjiang Province/ GR - SKLVBF202208/State Key Laboratory of Veterinary Biotechnology Foundation/ GR - 2022YFF0711004/National Key Research and Development Program/ GR - Not applicable/National Center of Technology Innovation for Pigs/ PT - Journal Article DEP - 20230809 PL - Switzerland TA - Vaccines (Basel) JT - Vaccines JID - 101629355 PMC - PMC10459522 OTO - NOTNLM OT - NADC30-like PRRSV OT - NADC34-like PRRSV OT - SD-R OT - cross-protection COIS- The authors declare no potential conflict of interest with respect to the research, authorship, or publication of this article. EDAT- 2023/08/26 10:44 MHDA- 2023/08/26 10:45 PMCR- 2023/08/09 CRDT- 2023/08/26 01:34 PHST- 2023/05/12 00:00 [received] PHST- 2023/08/04 00:00 [revised] PHST- 2023/08/07 00:00 [accepted] PHST- 2023/08/26 10:45 [medline] PHST- 2023/08/26 10:44 [pubmed] PHST- 2023/08/26 01:34 [entrez] PHST- 2023/08/09 00:00 [pmc-release] AID - vaccines11081349 [pii] AID - vaccines-11-01349 [pii] AID - 10.3390/vaccines11081349 [doi] PST - epublish SO - Vaccines (Basel). 2023 Aug 9;11(8):1349. doi: 10.3390/vaccines11081349.