PMID- 37632388
OWN - NLM
STAT- MEDLINE
DCOM- 20231005
LR  - 20231010
IS  - 1502-7686 (Electronic)
IS  - 0036-5513 (Linking)
VI  - 83
IP  - 6
DP  - 2023 Oct
TI  - Neurofilament light chain: serum reference intervals in Danish children aged 0-17 
      years.
PG  - 403-407
LID - 10.1080/00365513.2023.2251003 [doi]
AB  - Elevated levels of neurofilament light chain (NfL) in the blood is an unspecific 
      biomarker for damage to neuronal axons. The measurement of NfL levels in the 
      blood can provide useful information for monitoring and prognostication of 
      various neurological disorders in children, but a reference interval (RI) is 
      needed before the clinical implementation of the biomarker. We aimed to establish 
      a RI for children aged 0-17 years. Serum samples from 292 healthy reference 
      subjects aged 0.4-17.9 years were analysed by a single-molecule array (Simoa(R)) 
      established for routine clinical use. Non-parametric quantile regression was used 
      to model a continuous RI, and a traditional age-partitioned non-parametric RI was 
      established according to Clinical and Laboratory Standard Institute (CLSI) 
      guideline C28-A3. Furthermore, we investigated the effect of hemolysis on assay 
      performance. The traditional age-partitioned non-parametric RI for the age group 
      <3 years was 3.5-16.6 ng/L and 2.1-13.9 ng/L in the age group >/=3 years, 
      respectively. The continuous RI showed an age-dependent decrease in median NfL 
      levels in the first three years of life which was also evident in the 
      age-partitioning of the traditional RI. We found no difference between sexes and 
      no impact of hemolysis on the NfL test results. This study establishes a 
      pediatric RI for serum NfL and lays the groundwork for its future use in clinical 
      practice.
FAU - Schjorring, Mia Elbek
AU  - Schjorring ME
AD  - Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark.
FAU - Parkner, Tina
AU  - Parkner T
AD  - Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark.
AD  - Department of Clinical Medicine, Aarhus University, Denmark.
FAU - Knudsen, Cindy Soendersoe
AU  - Knudsen CS
AD  - Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark.
FAU - Tybirk, Lea
AU  - Tybirk L
AD  - Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark.
FAU - Hviid, Claus Vinter Bodker
AU  - Hviid CVB
AD  - Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark.
AD  - Department of Clinical Medicine, Aarhus University, Denmark.
AD  - Department of Clinical Biochemistry, Aalborg University Hospital, Denmark.
AD  - Department of Clinical Medicine, Aalborg University, Denmark.
LA  - eng
PT  - Journal Article
DEP - 20230826
PL  - England
TA  - Scand J Clin Lab Invest
JT  - Scandinavian journal of clinical and laboratory investigation
JID - 0404375
RN  - 0 (Biomarkers)
SB  - IM
MH  - Humans
MH  - Child
MH  - Adolescent
MH  - *Intermediate Filaments
MH  - *Hemolysis
MH  - Biomarkers
MH  - Denmark
OTO - NOTNLM
OT  - Neurofilament protein L
OT  - biomarker
OT  - children
OT  - hemolysis
OT  - reference interval
OT  - serum
EDAT- 2023/08/27 05:41
MHDA- 2023/10/05 06:44
CRDT- 2023/08/26 07:43
PHST- 2023/10/05 06:44 [medline]
PHST- 2023/08/27 05:41 [pubmed]
PHST- 2023/08/26 07:43 [entrez]
AID - 10.1080/00365513.2023.2251003 [doi]
PST - ppublish
SO  - Scand J Clin Lab Invest. 2023 Oct;83(6):403-407. doi: 
      10.1080/00365513.2023.2251003. Epub 2023 Aug 26.