PMID- 37634000
OWN - NLM
STAT- MEDLINE
DCOM- 20230828
LR  - 20231121
IS  - 1868-7083 (Electronic)
IS  - 1868-7075 (Print)
IS  - 1868-7075 (Linking)
VI  - 15
IP  - 1
DP  - 2023 Aug 26
TI  - Objective and subjective measures of sleep initiation are differentially 
      associated with DNA methylation in adolescents.
PG  - 136
LID - 10.1186/s13148-023-01553-2 [doi]
LID - 136
AB  - INTRODUCTION: The onset of puberty is associated with a shift in the circadian 
      timing of sleep, leading to delayed sleep initiation [i.e., later sleep onset 
      time (SOT)] due to later bedtimes and/or longer sleep onset latency (SOL). 
      Several genome-wide association studies (GWAS) have identified genes that may be 
      involved in the etiology of sleep phenotypes. However, circadian rhythms are also 
      epigenetically regulated; therefore, epigenetic biomarkers may provide insight 
      into the physiology of the pubertal sleep onset shift and the pathophysiology of 
      prolonged or delayed sleep initiation. RESULTS: The gene-wide analysis indicated 
      differential methylation within or around 1818 unique genes across the sleep 
      initiation measurements using self-report, actigraphy (ACT), and polysomnography 
      (PSG), while GWAS-informed analysis yielded 67 genes. Gene hits were identified 
      for bedtime (PSG), SOL (subjective, ACT and PSG) and SOT (subjective and PSG). 
      DNA methylation within 12 genes was associated with both subjective and 
      PSG-measured SOL, 31 with both ACT- and PSG-measured SOL, 19 with both subjective 
      and ACT-measured SOL, and one gene (SMG1P2) had methylation sites associated with 
      subjective, ACT- and PSG-measured SOL. CONCLUSIONS: Objective and subjective 
      sleep initiation in adolescents is associated with altered DNA methylation in 
      genes previously identified in adult GWAS of sleep and circadian phenotypes. 
      Additionally, our data provide evidence for a potential epigenetic link between 
      habitual (subjective and ACT) SOL and in-lab SOT and DNA methylation in and 
      around genes involved in circadian regulation (i.e., RASD1, RAI1), 
      cardiometabolic disorders (i.e., FADS1, WNK1, SLC5A6), and neuropsychiatric 
      disorders (i.e., PRR7, SDK1, FAM172A). If validated, these sites may provide 
      valuable targets for early detection and prevention of disorders involving 
      prolonged or delayed SOT, such as insomnia, delayed sleep phase, and their 
      comorbidity.
CI  - (c) 2023. BioMed Central Ltd., part of Springer Nature.
FAU - Larsen, Michael
AU  - Larsen M
AD  - Sleep Research and Treatment Center, Department of Psychiatry & Behavioral 
      Health, The Pennsylvania State University College of Medicine, Hershey, PA, 
      17033, USA.
FAU - He, Fan
AU  - He F
AD  - Department of Public Health Sciences, The Pennsylvania State University College 
      of Medicine, Hershey, PA, 17033, USA.
FAU - Kawasawa, Yuka Imamura
AU  - Kawasawa YI
AD  - Departments of Biochemistry and Molecular Biology and Pharmacology, Institute for 
      Personalized Medicine, The Pennsylvania State University College of Medicine, 
      Hershey, PA, 17033, USA.
FAU - Berg, Arthur
AU  - Berg A
AD  - Department of Public Health Sciences, The Pennsylvania State University College 
      of Medicine, Hershey, PA, 17033, USA.
FAU - Vgontzas, Alexandros N
AU  - Vgontzas AN
AD  - Sleep Research and Treatment Center, Department of Psychiatry & Behavioral 
      Health, The Pennsylvania State University College of Medicine, Hershey, PA, 
      17033, USA.
FAU - Liao, Duanping
AU  - Liao D
AD  - Department of Public Health Sciences, The Pennsylvania State University College 
      of Medicine, Hershey, PA, 17033, USA.
FAU - Bixler, Edward O
AU  - Bixler EO
AD  - Sleep Research and Treatment Center, Department of Psychiatry & Behavioral 
      Health, The Pennsylvania State University College of Medicine, Hershey, PA, 
      17033, USA.
FAU - Fernandez-Mendoza, Julio
AU  - Fernandez-Mendoza J
AD  - Sleep Research and Treatment Center, Department of Psychiatry & Behavioral 
      Health, The Pennsylvania State University College of Medicine, Hershey, PA, 
      17033, USA. jfmendoza@psu.edu.
LA  - eng
GR  - R01 MH118308/MH/NIMH NIH HHS/United States
GR  - R01 HL136587/HL/NHLBI NIH HHS/United States
GR  - R01 HL063772/HL/NHLBI NIH HHS/United States
GR  - R01 HL097165/HL/NHLBI NIH HHS/United States
GR  - UL1 TR000127/TR/NCATS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20230826
PL  - Germany
TA  - Clin Epigenetics
JT  - Clinical epigenetics
JID - 101516977
SB  - IM
MH  - *DNA Methylation
MH  - *Genome-Wide Association Study
MH  - Sexual Maturation
MH  - Sleep/genetics
MH  - Circadian Rhythm/genetics
PMC - PMC10464279
OTO - NOTNLM
OT  - Bedtime
OT  - DNA methylation
OT  - Epigenetics
OT  - Sleep initiation
OT  - Sleep latency
COIS- The authors declare that they have no competing interests.
EDAT- 2023/08/27 05:43
MHDA- 2023/08/28 06:42
PMCR- 2023/08/26
CRDT- 2023/08/26 23:25
PHST- 2023/01/19 00:00 [received]
PHST- 2023/08/14 00:00 [accepted]
PHST- 2023/08/28 06:42 [medline]
PHST- 2023/08/27 05:43 [pubmed]
PHST- 2023/08/26 23:25 [entrez]
PHST- 2023/08/26 00:00 [pmc-release]
AID - 10.1186/s13148-023-01553-2 [pii]
AID - 1553 [pii]
AID - 10.1186/s13148-023-01553-2 [doi]
PST - epublish
SO  - Clin Epigenetics. 2023 Aug 26;15(1):136. doi: 10.1186/s13148-023-01553-2.