PMID- 37634356
OWN - NLM
STAT- MEDLINE
DCOM- 20230911
LR  - 20230911
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 235
DP  - 2023 Oct 25
TI  - In vitro bloodbrain barrier permeability study of four main active ingredients 
      from Alpiniae oxyphyllae fructus.
PG  - 115637
LID - S0731-7085(23)00406-5 [pii]
LID - 10.1016/j.jpba.2023.115637 [doi]
AB  - The fruits of Alpinia oxyphylla Miq., a broadly utilized traditional Chinese 
      medicine, have a number of effects on the central nervous system (CNS). The main 
      active constituents of Alpiniae oxyphyllae fructus (AOF) were nootkatone, 
      tectochrysin, chrysin and protocatechuic acid. An immortalized human brain 
      microvascular endothelial cell (hCMEC/D3) and astrocyte (HA1800) coculture model 
      was used to investigate the permeability of the blood-brain barrier (BBB). The 
      validation of ultrahigh-performance liquid chromatography-tandem mass 
      spectrometry (UHPLC-MS/MS) methods for the four compounds was conducted following 
      industry guidelines. Calibration curves were generated with mean coefficients 
      (R(2)) better than 0.99. The inter-day and intra-day precisions were less than 
      8.53% and 7.12%, respectively. The accuracies were lower than +/- 11.57%, and 
      recoveries were greater than 86.07%. The samples of the transport experiment were 
      examined, and the apparent permeability coefficients (P(app)) were calculated. 
      The efflux ratios of the four compounds are all less than 2. The P(app) values of 
      protocatechuic acid, chrysin, nootkatone, tectochrysin were at the level of 
      10(-5), 10(-6), 10(-6), and 10(-7) cm/s, respectively. All four compounds crossed 
      the BBB by passive diffusion, with protocatechuic acid having high permeability, 
      and tectochrysin having poor permeability. This research indicated the 
      permeability of protocatechuic acid, chrysin, nootkatone and tectochrysin through 
      the BBB and offered a foundation for related research on AOF in the treatment of 
      CNS illnesses.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Xiao, Tongjie
AU  - Xiao T
AD  - Key Laboratory of Tropical Biological Resources of Ministry of Education, School 
      of Pharmaceutical Sciences, Hainan University, Haikou 570228, China.
FAU - Pan, Mingyu
AU  - Pan M
AD  - Key Laboratory of Tropical Biological Resources of Ministry of Education, School 
      of Pharmaceutical Sciences, Hainan University, Haikou 570228, China.
FAU - Wang, Yuanxiao
AU  - Wang Y
AD  - Key Laboratory of Tropical Biological Resources of Ministry of Education, School 
      of Pharmaceutical Sciences, Hainan University, Haikou 570228, China.
FAU - Huang, Yanjiao
AU  - Huang Y
AD  - Key Laboratory of Tropical Biological Resources of Ministry of Education, School 
      of Pharmaceutical Sciences, Hainan University, Haikou 570228, China.
FAU - Tsunoda, Makoto
AU  - Tsunoda M
AD  - Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, 
      Bunkyo-ku, Tokyo 113-0033, Japan.
FAU - Zhang, Yingxia
AU  - Zhang Y
AD  - Key Laboratory of Tropical Biological Resources of Ministry of Education, School 
      of Pharmaceutical Sciences, Hainan University, Haikou 570228, China.
FAU - Wang, Rong
AU  - Wang R
AD  - Key Laboratory of Tropical Biological Resources of Ministry of Education, School 
      of Pharmaceutical Sciences, Hainan University, Haikou 570228, China.
FAU - Hu, Wenting
AU  - Hu W
AD  - Key Laboratory of Tropical Biological Resources of Ministry of Education, School 
      of Pharmaceutical Sciences, Hainan University, Haikou 570228, China.
FAU - Yang, Haimei
AU  - Yang H
AD  - Key Laboratory of Tropical Biological Resources of Ministry of Education, School 
      of Pharmaceutical Sciences, Hainan University, Haikou 570228, China. Electronic 
      address: haimeiy@hainanu.edu.cn.
FAU - Li, Lu-Shuang
AU  - Li LS
AD  - Key Laboratory of Tropical Biological Resources of Ministry of Education, School 
      of Pharmaceutical Sciences, Hainan University, Haikou 570228, China. Electronic 
      address: lvylushuang@163.com.
FAU - Song, Yanting
AU  - Song Y
AD  - Key Laboratory of Tropical Biological Resources of Ministry of Education, School 
      of Pharmaceutical Sciences, Hainan University, Haikou 570228, China. Electronic 
      address: songyt@hainanu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20230811
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - IZ2Y119N4J (nootkatone)
RN  - 36R5QJ8L4B (protocatechuic acid)
SB  - IM
MH  - Humans
MH  - *Blood-Brain Barrier
MH  - *Fruit
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Alpiniae oxyphyllae fructus
OT  - Blood3brain barrier
OT  - Permeability
COIS- Declaration of Competing Interest The authors declare that they have no conflicts 
      of interest.
EDAT- 2023/08/28 00:41
MHDA- 2023/09/11 06:42
CRDT- 2023/08/27 18:05
PHST- 2023/05/13 00:00 [received]
PHST- 2023/07/15 00:00 [revised]
PHST- 2023/08/04 00:00 [accepted]
PHST- 2023/09/11 06:42 [medline]
PHST- 2023/08/28 00:41 [pubmed]
PHST- 2023/08/27 18:05 [entrez]
AID - S0731-7085(23)00406-5 [pii]
AID - 10.1016/j.jpba.2023.115637 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2023 Oct 25;235:115637. doi: 10.1016/j.jpba.2023.115637. 
      Epub 2023 Aug 11.