PMID- 37634410
OWN - NLM
STAT- MEDLINE
DCOM- 20230919
LR  - 20230921
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 678
DP  - 2023 Oct 20
TI  - MCTS1 enhances the proliferation of laryngeal squamous cell carcinoma via 
      promoting OTUD6B-1 mediated LIN28B deubiquitination.
PG  - 128-134
LID - S0006-291X(23)00982-8 [pii]
LID - 10.1016/j.bbrc.2023.08.036 [doi]
AB  - The aberrant upregulation of MCTS1 Re-Initiation and Release Factor (also known 
      as Malignant T-cell-amplified sequence 1, MCTS1) can promote laryngeal squamous 
      cell carcinoma (LSCC). It might act as a binding partner of multiple proteins. In 
      this study, we further explored the expression of potential interaction between 
      MCTS1 and OTU domain-containing protein 6B (OTUD6B) and its influence on the 
      ubiquitination and degradation of OTUD6B's substrate in LSCC. LSCC cell lines 
      AMC-HN-8 and TU177 were utilized for assessing protein-protein interaction, 
      protein degradation and tumor growth in vitro and in vivo. The results showed 
      that MCTS1 interacts with OUTD6B isoform 1 (OTUD6B-1) in the cell lines. Higher 
      OTUD6B-1 expression is associated with significantly shorter progression-free 
      interval in LSCC patients. OTUD6B positively modulated the expression of cyclin 
      D1, cyclin E1 and c-Myc and LSCC cell proliferation in vitro and in vivo. MCTS1 
      negatively modulated the degradation of LIN28B in G1/S cells, via enhancing 
      OTUD6B-mediated cleaving of K48-branched ubiquitin chains from LIN28B. OTUD6B or 
      LIN28B shRNA weakened MCTS1 overexpression-induced cyclin D1 and c-Myc protein 
      expression and LSCC cell proliferation. In summary, this study revealed that 
      MCTS1 could enhance LSCC proliferation partially via the OTUD6B-LIN28B axis.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Ma, Binjuan
AU  - Ma B
AD  - Otorhinolaryngology-Head and Neck Surgery, Gansu Provincial Hospital, Lanzhou, 
      China.
FAU - Wei, Xiaoquan
AU  - Wei X
AD  - Otorhinolaryngology-Head and Neck Surgery, Gansu Provincial Hospital, Lanzhou, 
      China.
FAU - Zhou, Shijie
AU  - Zhou S
AD  - State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, 
      Sichuan University, Chengdu, China.
FAU - Yang, Mengsheng
AU  - Yang M
AD  - Otorhinolaryngology-Head and Neck Surgery, Gansu Provincial Hospital, Lanzhou, 
      China. Electronic address: yang76120687@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230818
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Cell Cycle Proteins)
RN  - 136601-57-5 (Cyclin D1)
RN  - 0 (LIN28B protein, human)
RN  - 0 (MCTS1 protein, human)
RN  - 0 (Oncogene Proteins)
RN  - 0 (RNA-Binding Proteins)
RN  - EC 3.4.- (OTUD6B protein, human)
SB  - IM
MH  - Humans
MH  - Cell Cycle Proteins
MH  - Cell Line
MH  - Cell Proliferation
MH  - *Cyclin D1
MH  - *Head and Neck Neoplasms
MH  - Oncogene Proteins
MH  - RNA-Binding Proteins
MH  - Squamous Cell Carcinoma of Head and Neck/genetics
OTO - NOTNLM
OT  - LIN28B
OT  - Laryngeal squamous cell carcinoma
OT  - MCTS1
OT  - OTUD6B
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/08/28 00:41
MHDA- 2023/09/18 12:42
CRDT- 2023/08/27 18:07
PHST- 2023/08/07 00:00 [received]
PHST- 2023/08/16 00:00 [accepted]
PHST- 2023/09/18 12:42 [medline]
PHST- 2023/08/28 00:41 [pubmed]
PHST- 2023/08/27 18:07 [entrez]
AID - S0006-291X(23)00982-8 [pii]
AID - 10.1016/j.bbrc.2023.08.036 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2023 Oct 20;678:128-134. doi: 
      10.1016/j.bbrc.2023.08.036. Epub 2023 Aug 18.