PMID- 37648885
OWN - NLM
STAT- MEDLINE
DCOM- 20230901
LR  - 20231123
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 13
IP  - 1
DP  - 2023 Aug 30
TI  - Single-cell RNA sequencing to detect age-associated genes that identify senescent 
      cells in the liver of aged mice.
PG  - 14186
LID - 10.1038/s41598-023-41352-6 [doi]
LID - 14186
AB  - Senescent cells are predicted to occur and increase in animal tissues with aging. 
      However, senescent cells in the tissues of aged animals remain to be identified. 
      We refer to the marker genes to identify senescent cells in tissues as 
      "age-associated genes". In this study, we searched for age-associated genes to 
      identify senescent cells in the livers of aged animals. We performed single-cell 
      RNA sequencing (scRNA-seq) to screen candidates for age-associated genes using 
      young and aged rat primary hepatocytes. To remove animal species specificity, 
      gene expression analyses in mouse livers were performed, confirming 
      age-associated increases in the mRNA expression levels of Glipr1, Clec12a, and 
      Phlda3. Moreover, the mRNA expression levels of Glipr1 and Phlda3 were increased 
      by stress-induced premature senescence using doxorubicin in primary hepatocytes 
      and livers of young mice. Transcriptome data of aged rat hepatocytes suggested 
      that Glipr1, Clec12a, and Phlda3 were expressed in almost identical cells. 
      Fluorescence in situ hybridization (FISH) confirmed the presence of cells with 
      abundant Glipr1, Clec12a, and Phlda3 mRNA in 27-month-old mouse primary 
      hepatocytes, which are considered to be senescent cells. This study is the first 
      to identify Glipr1, Clec12a, and Phlda3 as age-associated genes in the mouse 
      liver.
CI  - (c) 2023. Springer Nature Limited.
FAU - Doshida, Yuta
AU  - Doshida Y
AD  - Molecular Regulation of Aging, Tokyo Metropolitan Institute for Geriatrics and 
      Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo, 173-0015, Japan.
AD  - Department of Biological Sciences, Tokyo Metropolitan University, Tokyo, 
      192-0397, Japan.
FAU - Hashimoto, Shinichi
AU  - Hashimoto S
AD  - Department of Molecular Pathophysiology, Institute of Advanced Medicine, Wakayama 
      Medical University, Wakayama, 641-8509, Japan.
FAU - Iwabuchi, Sadahiro
AU  - Iwabuchi S
AD  - Department of Molecular Pathophysiology, Institute of Advanced Medicine, Wakayama 
      Medical University, Wakayama, 641-8509, Japan.
FAU - Takino, Yuka
AU  - Takino Y
AD  - Molecular Regulation of Aging, Tokyo Metropolitan Institute for Geriatrics and 
      Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo, 173-0015, Japan.
FAU - Ishiwata, Toshiyuki
AU  - Ishiwata T
AD  - Aging and Carcinogenesis, Tokyo Metropolitan Institute for Geriatrics and 
      Gerontology, Tokyo, 173-0015, Japan.
FAU - Aigaki, Toshiro
AU  - Aigaki T
AD  - Department of Biological Sciences, Tokyo Metropolitan University, Tokyo, 
      192-0397, Japan.
FAU - Ishigami, Akihito
AU  - Ishigami A
AD  - Molecular Regulation of Aging, Tokyo Metropolitan Institute for Geriatrics and 
      Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo, 173-0015, Japan. 
      ishigami@tmig.or.jp.
AD  - Department of Biological Sciences, Tokyo Metropolitan University, Tokyo, 
      192-0397, Japan. ishigami@tmig.or.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230830
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Mice
MH  - Rats
MH  - Animals
MH  - In Situ Hybridization, Fluorescence
MH  - *Liver
MH  - *Cellular Senescence/genetics
MH  - RNA, Messenger/genetics
MH  - Sequence Analysis, RNA
PMC - PMC10468526
COIS- The authors declare no competing interests.
EDAT- 2023/08/31 00:41
MHDA- 2023/09/01 06:43
PMCR- 2023/08/30
CRDT- 2023/08/30 23:32
PHST- 2022/12/23 00:00 [received]
PHST- 2023/08/24 00:00 [accepted]
PHST- 2023/09/01 06:43 [medline]
PHST- 2023/08/31 00:41 [pubmed]
PHST- 2023/08/30 23:32 [entrez]
PHST- 2023/08/30 00:00 [pmc-release]
AID - 10.1038/s41598-023-41352-6 [pii]
AID - 41352 [pii]
AID - 10.1038/s41598-023-41352-6 [doi]
PST - epublish
SO  - Sci Rep. 2023 Aug 30;13(1):14186. doi: 10.1038/s41598-023-41352-6.