PMID- 37649778
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230907
IS  - 2753-149X (Electronic)
IS  - 2753-149X (Linking)
VI  - 2
DP  - 2023
TI  - Dynamics and Mechanisms of ERK Activation after Different Protocols that Induce 
      Long-Term Synaptic Facilitation in Aplysia.
PG  - kvac014
LID - 10.1093/oons/kvac014 [doi]
LID - kvac014
AB  - Phosphorylation of the MAPK family member extracellular signal-regulated kinase 
      (ERK) is required to induce long-term synaptic plasticity, but little is known 
      about its persistence. We examined ERK activation by three protocols that induce 
      long-term synaptic facilitation (LTF) of the Aplysia sensorimotor synapse - the 
      standard protocol (five 5-min pulses of 5-HT with interstimulus intervals (ISIs) 
      of 20 min), the enhanced protocol (five pulses with irregular ISIs, which induces 
      greater and longer-lasting LTF) and the two-pulse protocol (two pulses with ISI 
      45 min). Immunofluorescence revealed complex ERK activation. The standard and 
      two-pulse protocols immediately increased active, phosphorylated ERK (pERK), 
      which decayed within 5 h. A second wave of increased pERK was detected 18 h 
      post-treatment for all protocols. This late phase was blocked by inhibitors of 
      protein kinase A, TrkB and TGF-beta. These results suggest that complex interactions 
      among kinase pathways and growth factors contribute to the late increase of pERK. 
      ERK activity returned to basal 24 h after the standard or two-pulse protocols, 
      but remained elevated 24 h for the enhanced protocol. This 24-h elevation was 
      also dependent on PKA and TGF-beta, and partly on TrkB. These results begin to 
      characterize long-lasting ERK activation, plausibly maintained by positive 
      feedback involving growth factors and PKA, that appears essential to maintain LTF 
      and LTM. Because many processes involved in LTF and late LTP are conserved among 
      Aplysia and mammals, these findings highlight the importance of examining the 
      dynamics of kinase cascades involved in vertebrate long-term memory.
CI  - (c) The Author(s) 2022. Published by Oxford University Press.
FAU - Zhang, Yili
AU  - Zhang Y
AUID- ORCID: 0000-0002-2591-1324
AD  - Department of Neurobiology and Anatomy, W.M. Keck Center for the Neurobiology of 
      Learning and Memory, McGovern Medical School at the University of Texas Health 
      Science Center at Houston, 6431 Fannin Street, Suite MSB 7.046, Houston, TX 
      77030, United States.
FAU - Liu, Rong-Yu
AU  - Liu RY
AD  - Department of Neurobiology and Anatomy, W.M. Keck Center for the Neurobiology of 
      Learning and Memory, McGovern Medical School at the University of Texas Health 
      Science Center at Houston, 6431 Fannin Street, Suite MSB 7.046, Houston, TX 
      77030, United States.
FAU - Smolen, Paul
AU  - Smolen P
AD  - Department of Neurobiology and Anatomy, W.M. Keck Center for the Neurobiology of 
      Learning and Memory, McGovern Medical School at the University of Texas Health 
      Science Center at Houston, 6431 Fannin Street, Suite MSB 7.046, Houston, TX 
      77030, United States.
FAU - Cleary, Leonard J
AU  - Cleary LJ
AD  - Department of Neurobiology and Anatomy, W.M. Keck Center for the Neurobiology of 
      Learning and Memory, McGovern Medical School at the University of Texas Health 
      Science Center at Houston, 6431 Fannin Street, Suite MSB 7.046, Houston, TX 
      77030, United States.
FAU - Byrne, John H
AU  - Byrne JH
AD  - Department of Neurobiology and Anatomy, W.M. Keck Center for the Neurobiology of 
      Learning and Memory, McGovern Medical School at the University of Texas Health 
      Science Center at Houston, 6431 Fannin Street, Suite MSB 7.046, Houston, TX 
      77030, United States.
LA  - eng
GR  - R01 NS019895/NS/NINDS NIH HHS/United States
GR  - R37 NS019895/NS/NINDS NIH HHS/United States
PT  - Journal Article
DEP - 20221018
PL  - England
TA  - Oxf Open Neurosci
JT  - Oxford open neuroscience
JID - 9918487584406676
PMC - PMC10464504
OTO - NOTNLM
OT  - Aplysia
OT  - ERK
OT  - TGF-beta
OT  - TrkB
OT  - computationally designed training protocol
OT  - long-term facilitation
COIS- None declared.
EDAT- 2023/08/31 06:42
MHDA- 2023/08/31 06:43
PMCR- 2022/10/18
CRDT- 2023/08/31 04:04
PHST- 2022/08/22 00:00 [received]
PHST- 2022/10/05 00:00 [revised]
PHST- 2023/08/31 06:43 [medline]
PHST- 2023/08/31 06:42 [pubmed]
PHST- 2023/08/31 04:04 [entrez]
PHST- 2022/10/18 00:00 [pmc-release]
AID - kvac014 [pii]
AID - 10.1093/oons/kvac014 [doi]
PST - epublish
SO  - Oxf Open Neurosci. 2022 Oct 18;2:kvac014. doi: 10.1093/oons/kvac014. eCollection 
      2023.