PMID- 37650730
OWN - NLM
STAT- MEDLINE
DCOM- 20230901
LR  - 20230912
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Linking)
VI  - 104
IP  - 8
DP  - 2023 Aug
TI  - A novel chimeric vaccine candidate for porcine reproductive and respiratory 
      syndrome virus (PRRSV) I and II elicits neutralizing antibodies against both 
      types.
LID - 10.1099/jgv.0.001889 [doi]
AB  - Porcine reproductive and respiratory syndrome virus (PRRSV) is an economically 
      important virus within the swine industry. The virus causes respiratory disease 
      and reproductive failure. Two species of PRRSV-I and II are co-dominant, yet no 
      effective vaccination strategy has been developed to protect against these two 
      types. With an aim to develop a chimeric vaccine strain to protect against both 
      types, in this study, a chimeric porcine reproductive and respiratory syndrome 
      virus (PRRSV) type I and II was rescued using reverse genetics for the first 
      time. Four chimeric infectious clones were designed based on the genomic 
      arrangement of the structural proteins. However, only the clone carrying the 
      transcriptional regulatory sequence (TRS) and ORF6 of a PRRSV-I and ORF6 of a 
      PRRSV-II generated a viable recombinant virus, suggesting that concurrent 
      expression of ORF6 from both parental viruses is essential for the recovery of 
      type I and II chimeric PRRSV. The chimeric virus showed significantly lower 
      replication ability than its parental strains in vitro, which was improved by 
      serial passaging. In vivo, groups of pigs were inoculated with either the 
      chimeric virus, one of the parental strains, or PBS. The chimeric virus 
      replicated in pig tissue and was detected in serum 7 days post-inoculation. Serum 
      neutralization tests indicated that pigs inoculated with the chimeric virus 
      elicited neutralizing antibodies that inhibited infection with strains of both 
      species and with greater coverage than the parental viruses. In conclusion, the 
      application of this technique to construct a chimeric PRRSV holds promise for the 
      development of a highly effective modified live vaccine candidate. This is 
      particularly significant since there are currently no approved commercial 
      divalent vaccines available to combat PRRSV-I and II co-infections.
FAU - Cha, Sang-Ho
AU  - Cha SH
AD  - PRRS Research Laboratory, Viral Diseases Division, Animal and Plant Quarantine 
      Agency, Gimcheon, 39660, Republic of Korea.
FAU - Hyun, Bang-Hun
AU  - Hyun BH
AD  - PRRS Research Laboratory, Viral Diseases Division, Animal and Plant Quarantine 
      Agency, Gimcheon, 39660, Republic of Korea.
FAU - Lee, Hyang-Sim
AU  - Lee HS
AD  - PRRS Research Laboratory, Viral Diseases Division, Animal and Plant Quarantine 
      Agency, Gimcheon, 39660, Republic of Korea.
FAU - Kang, Seok-Jin
AU  - Kang SJ
AD  - PRRS Research Laboratory, Viral Diseases Division, Animal and Plant Quarantine 
      Agency, Gimcheon, 39660, Republic of Korea.
FAU - You, Su-Hwa
AU  - You SH
AD  - PRRS Research Laboratory, Viral Diseases Division, Animal and Plant Quarantine 
      Agency, Gimcheon, 39660, Republic of Korea.
FAU - Jeong, Jiwoon
AU  - Jeong J
AD  - Division of Animal Care, Yonam College, 313, Yeonam-ro, Seonghwan-eup, Seobuk-gu, 
      Cheonan-si, Chungcheongnam-do, Republic of Korea.
FAU - Park, Chang-Joo
AU  - Park CJ
AD  - Department of Microbiology and Immunology, Eulji University School of Medicine, 
      Yongdu-dong, Jung-gu, Daejeon, Republic of Korea.
FAU - Lee, Myung-Shin
AU  - Lee MS
AD  - Department of Microbiology and Immunology, Eulji University School of Medicine, 
      Yongdu-dong, Jung-gu, Daejeon, Republic of Korea.
FAU - Park, Changhoon
AU  - Park C
AD  - Department of Microbiology and Immunology, Eulji University School of Medicine, 
      Yongdu-dong, Jung-gu, Daejeon, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Vaccines, Attenuated)
SB  - IM
MH  - Animals
MH  - Swine
MH  - Antibodies, Neutralizing
MH  - *Porcine respiratory and reproductive syndrome virus/genetics
MH  - Vaccination
MH  - Vaccines, Attenuated/genetics
MH  - *Coinfection
OTO - NOTNLM
OT  - chimera
OT  - porcine reproductive and respiratory syndrome virus
OT  - reverse genetics
OT  - vaccine
EDAT- 2023/08/31 12:42
MHDA- 2023/09/01 06:43
CRDT- 2023/08/31 09:53
PHST- 2023/09/01 06:43 [medline]
PHST- 2023/08/31 12:42 [pubmed]
PHST- 2023/08/31 09:53 [entrez]
AID - 10.1099/jgv.0.001889 [doi]
PST - ppublish
SO  - J Gen Virol. 2023 Aug;104(8). doi: 10.1099/jgv.0.001889.