PMID- 37652426
OWN - NLM
STAT- MEDLINE
DCOM- 20231204
LR  - 20231204
IS  - 1078-6791 (Print)
IS  - 1078-6791 (Linking)
VI  - 29
IP  - 8
DP  - 2023 Nov
TI  - Regulation of M1/M2 Polarization in LPS-Stimulated Macrophages by 1,25(OH)2D3.
PG  - 501-505
LID - AT8641 [pii]
AB  - OBJECTIVE: This study aims to investigate the impact of 1,25(OH)2D3 on the 
      polarization of LPS-stimulated macrophages and the underlying regulatory 
      mechanisms. METHODS: Primary macrophages were isolated and identified using 
      immunofluorescence assays to detect macrophage biomarker expression levels. 
      RT-PCR was employed to measure the expression of Arginase 1 (Arg-1), 
      Interleukin-10 (IL-10), Inducible isoform of nitric oxide synthase (iNOS), and 
      Tumor necrosis factor-alpha (TNF-alpha) in macrophages treated with various strategies. 
      Western blotting assessed the protein expression levels of AKT1, p-AKT1, NF-kappaB 
      p65, p-NF-kappaB p65, STAT3, and p-STAT3 in LPS-stimulated macrophages exposed to 
      different concentrations of 1,25(OH)2D3. RESULTS: As the LPS concentration 
      increased from 0 to 0.5 mg/L, Arg-1, IL-10, iNOS, and TNF-alpha expression levels 
      significantly increased. However, at LPS concentrations ranging from 1 mg/L to 10 
      mg/L, the expression of Arg-1, IL-10, iNOS, and TNF-alpha displayed a trend from 
      increase to decline. The highest M2 polarization (Arg-1 and IL-10) was observed 
      in macrophages stimulated with 0.5 mg/L LPS among the lower concentrations, while 
      the highest M1 polarization (iNOS and TNF-alpha) was observed in macrophages 
      stimulated with 5 mg/L LPS among the higher concentrations. Subsequent 
      experiments utilized 0.5 mg/L and 5 mg/L LPS as incubation concentrations. Under 
      LPS stimulation, iNOS was significantly upregulated, surpassing the expression 
      level of IL-10, a marker of M2 macrophages. The introduction of 1,25(OH)2D3 
      facilitated M2 polarization, with 50 nM as the incubation concentration of 
      1,25(OH)2D3. Furthermore, 1,25(OH)2D3 reversed the elevated expression of p-AKT1, 
      p-NF-kappaB p65, and p-STAT3 in macrophages stimulated with 5 mg/L LPS. CONCLUSIONS: 
      1,25(OH)2D3 effectively regulates the M1/M2 polarization in LPS-stimulated 
      macrophages.
FAU - Zhen, Hong
AU  - Zhen H
FAU - Hu, Hongbo
AU  - Hu H
FAU - Tan, Chang
AU  - Tan C
FAU - Yu, Xinyuan
AU  - Yu X
FAU - Gan, Xinglin
AU  - Gan X
FAU - Huang, Xiuxiu
AU  - Huang X
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Altern Ther Health Med
JT  - Alternative therapies in health and medicine
JID - 9502013
RN  - 130068-27-8 (Interleukin-10)
RN  - 0 (Lipopolysaccharides)
RN  - FXC9231JVH (Calcitriol)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Humans
MH  - *Interleukin-10/metabolism
MH  - *Lipopolysaccharides/pharmacology/metabolism
MH  - Calcitriol/metabolism
MH  - NF-kappa B/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Macrophages/metabolism
EDAT- 2023/09/01 00:41
MHDA- 2023/12/04 12:42
CRDT- 2023/08/31 19:42
PHST- 2023/12/04 12:42 [medline]
PHST- 2023/09/01 00:41 [pubmed]
PHST- 2023/08/31 19:42 [entrez]
AID - AT8641 [pii]
PST - ppublish
SO  - Altern Ther Health Med. 2023 Nov;29(8):501-505.