PMID- 37653354
OWN - NLM
STAT- Publisher
LR  - 20230831
IS  - 1433-8491 (Electronic)
IS  - 0940-1334 (Linking)
DP  - 2023 Sep 1
TI  - The atypical antidepressant tianeptine confers neuroprotection against 
      oxygen-glucose deprivation.
LID - 10.1007/s00406-023-01685-9 [doi]
AB  - Proregenerative and neuroprotective effects of antidepressants are an important 
      topic of inquiry in neuropsychiatric research. Oxygen-glucose deprivation (OGD) 
      mimics key aspects of ischemic injury in vitro. Here, we studied the effects of 
      24-h pretreatment with serotonin (5-HT), citalopram (CIT), fluoxetine (FLU), and 
      tianeptine (TIA) on primary mouse cortical neurons subjected to transient OGD. 
      5-HT (50 muM) significantly enhanced neuron viability as measured by MTT assay and 
      reduced cell death and LDH release. CIT (10 muM) and FLU (1 muM) did not increase 
      the effects of 5-HT and neither antidepressant conferred neuroprotection in the 
      absence of supplemental 5-HT in serum-free cell culture medium. By contrast, 
      pre-treatment with TIA (10 muM) resulted in robust neuroprotection, even in the 
      absence of 5-HT. Furthermore, TIA inhibited mRNA transcription of candidate genes 
      related to cell death and hypoxia and attenuated lipid peroxidation, a hallmark 
      of neuronal injury. Finally, deep RNA sequencing of primary neurons subjected to 
      OGD demonstrated that OGD induces many pathways relating to cell survival, the 
      inflammation-immune response, synaptic dysregulation and apoptosis, and that TIA 
      pretreatment counteracted these effects of OGD. In conclusion, this study 
      highlights the comparative strength of the 5-HT independent neuroprotective 
      effects of TIA and identifies the molecular pathways involved.
CI  - (c) 2023. The Author(s).
FAU - Ersoy, Burcu
AU  - Ersoy B
AD  - Department of Neurology, Charite-Universitatsmedizin Berlin, Corporate Member of 
      Freie Universitat Berlin and Humboldt-Universitat zu Berlin, Berlin, Germany.
AD  - Center for Stroke Research Berlin, Department of Experimental Neurology, 
      Charite-Universitatsmedizin Berlin, Corporate member of Freie Universitat Berlin 
      and Humboldt-Universitat zu Berlin, Berlin, Germany.
AD  - Berlin-Brandenburg School for Regenerative Therapies, Charite-Universitatsmedizin 
      Berlin, Berlin, Germany.
FAU - Herzog, Marie-Louise
AU  - Herzog ML
AD  - Department of Neurology, Charite-Universitatsmedizin Berlin, Corporate Member of 
      Freie Universitat Berlin and Humboldt-Universitat zu Berlin, Berlin, Germany.
AD  - Center for Stroke Research Berlin, Department of Experimental Neurology, 
      Charite-Universitatsmedizin Berlin, Corporate member of Freie Universitat Berlin 
      and Humboldt-Universitat zu Berlin, Berlin, Germany.
AD  - DZHK (German Center for Cardiovascular Research), Partner site, Berlin, Germany.
FAU - Pan, Wen
AU  - Pan W
AD  - Department of Neurology, Charite-Universitatsmedizin Berlin, Corporate Member of 
      Freie Universitat Berlin and Humboldt-Universitat zu Berlin, Berlin, Germany.
AD  - Center for Stroke Research Berlin, Department of Experimental Neurology, 
      Charite-Universitatsmedizin Berlin, Corporate member of Freie Universitat Berlin 
      and Humboldt-Universitat zu Berlin, Berlin, Germany.
AD  - DZHK (German Center for Cardiovascular Research), Partner site, Berlin, Germany.
FAU - Schilling, Simone
AU  - Schilling S
AD  - Department of Neurology, Charite-Universitatsmedizin Berlin, Corporate Member of 
      Freie Universitat Berlin and Humboldt-Universitat zu Berlin, Berlin, Germany.
AD  - Center for Stroke Research Berlin, Department of Experimental Neurology, 
      Charite-Universitatsmedizin Berlin, Corporate member of Freie Universitat Berlin 
      and Humboldt-Universitat zu Berlin, Berlin, Germany.
AD  - DZHK (German Center for Cardiovascular Research), Partner site, Berlin, Germany.
AD  - Berlin Institute of Health at Charite, Universitatsmedizin Berlin, Berlin, 
      Germany.
FAU - Endres, Matthias
AU  - Endres M
AD  - Department of Neurology, Charite-Universitatsmedizin Berlin, Corporate Member of 
      Freie Universitat Berlin and Humboldt-Universitat zu Berlin, Berlin, Germany.
AD  - Center for Stroke Research Berlin, Department of Experimental Neurology, 
      Charite-Universitatsmedizin Berlin, Corporate member of Freie Universitat Berlin 
      and Humboldt-Universitat zu Berlin, Berlin, Germany.
AD  - DZHK (German Center for Cardiovascular Research), Partner site, Berlin, Germany.
AD  - Einstein Center for Neurosciences, Charite-Universitatsmedizin Berlin, Berlin, 
      Germany.
AD  - DZNE (German Center for Neurodegenerative Diseases), Partner site, Berlin, 
      Germany.
AD  - DZPG (German Center for Mental Health), Partner site, Berlin, Germany.
FAU - Gottert, Ria
AU  - Gottert R
AD  - Department of Neurology, Charite-Universitatsmedizin Berlin, Corporate Member of 
      Freie Universitat Berlin and Humboldt-Universitat zu Berlin, Berlin, Germany.
AD  - Center for Stroke Research Berlin, Department of Experimental Neurology, 
      Charite-Universitatsmedizin Berlin, Corporate member of Freie Universitat Berlin 
      and Humboldt-Universitat zu Berlin, Berlin, Germany.
AD  - DZHK (German Center for Cardiovascular Research), Partner site, Berlin, Germany.
FAU - Kronenberg, Golo D
AU  - Kronenberg GD
AD  - Department of Psychiatry, Psychotherapy and Psychosomatics, University Hospital 
      of Psychiatry Zurich, Lenggstrasse 31, P.O. Box 363, 8032, Zurich, Switzerland.
FAU - Gertz, Karen
AU  - Gertz K
AUID- ORCID: 0000-0002-1203-641X
AD  - Department of Neurology, Charite-Universitatsmedizin Berlin, Corporate Member of 
      Freie Universitat Berlin and Humboldt-Universitat zu Berlin, Berlin, Germany. 
      karen.gertz@charite.de.
AD  - Center for Stroke Research Berlin, Department of Experimental Neurology, 
      Charite-Universitatsmedizin Berlin, Corporate member of Freie Universitat Berlin 
      and Humboldt-Universitat zu Berlin, Berlin, Germany. karen.gertz@charite.de.
AD  - DZHK (German Center for Cardiovascular Research), Partner site, Berlin, Germany. 
      karen.gertz@charite.de.
AD  - Einstein Center for Neurosciences, Charite-Universitatsmedizin Berlin, Berlin, 
      Germany. karen.gertz@charite.de.
LA  - eng
GR  - EXC-2049 - 390688087/Deutsche Forschungsgemeinschaft/
GR  - ReTune TRR 295- 424778381/Deutsche Forschungsgemeinschaft/
GR  - KR 2956/4-1/Deutsche Forschungsgemeinschaft/
GR  - GE 2576/3-1/Deutsche Forschungsgemeinschaft/
PT  - Journal Article
DEP - 20230901
PL  - Germany
TA  - Eur Arch Psychiatry Clin Neurosci
JT  - European archives of psychiatry and clinical neuroscience
JID - 9103030
SB  - IM
OTO - NOTNLM
OT  - Ischemia
OT  - Neuroprotection
OT  - Serotonin
OT  - Stroke
OT  - Tianeptine
EDAT- 2023/09/01 00:41
MHDA- 2023/09/01 00:41
CRDT- 2023/08/31 23:42
PHST- 2023/06/17 00:00 [received]
PHST- 2023/08/14 00:00 [accepted]
PHST- 2023/09/01 00:41 [medline]
PHST- 2023/09/01 00:41 [pubmed]
PHST- 2023/08/31 23:42 [entrez]
AID - 10.1007/s00406-023-01685-9 [pii]
AID - 10.1007/s00406-023-01685-9 [doi]
PST - aheadofprint
SO  - Eur Arch Psychiatry Clin Neurosci. 2023 Sep 1. doi: 10.1007/s00406-023-01685-9.