PMID- 37654976
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230905
IS  - 2575-9612 (Electronic)
IS  - 2575-9612 (Linking)
VI  - 7
IP  - 3
DP  - 2023
TI  - Elevated Maternal Testosterone Levels Alter PFOA Elimination and Tissue 
      Distribution in Pregnant Rats.
PG  - 131-139
LID - 10.26502/jesph.96120193 [doi]
AB  - Perfluorooctanoic acid (PFOA) is an enduring synthetic chemical that harms human 
      health. Recent studies indicate heightened bioaccumulation of PFOA, particularly 
      in pregnant women experiencing preeclampsia. Since plasma testosterone levels are 
      elevated in pregnant women with preeclampsia, we hypothesized that 
      hyperandrogenic conditions during pregnancy may hinder PFOA elimination and 
      contribute to their higher body burden. Pregnant Sprague-Dawley rats were s/c 
      injected with vehicle or testosterone propionate from gestational day (GD) 15 to 
      20 to increase plasma testosterone levels by 2-fold, similar to levels in 
      preeclampsia. On GD 16, [(14)C]-PFOA (9.4 pmol/kg) was given intravenously, and 
      subsequently, (14)C radioactivity was measured in maternal blood, urine, feces, 
      and tissues. PFOA was primarily eliminated through urine; however, less PFOA was 
      excreted in urine of pregnant rats with elevated testosterone levels than 
      controls. Fecal excretion of PFOA was minimal and did not significantly differ 
      between groups. The total elimination of PFOA (urine plus feces) was 
      significantly reduced by 12% in pregnant rats with elevated testosterone levels. 
      In controls, PFOA distribution was highest in placenta, followed by the kidneys, 
      liver, brain, heart, lungs, and spleen. Pregnant rats with elevated testosterone 
      levels displayed 12% higher concentrations of PFOA in these tissues than 
      controls. Furthermore, the renal expression of Oat2 and Oat3 was significantly 
      decreased, while Oatp1 and Oat-k expression was significantly increased in 
      pregnant rats with elevated testosterone levels than controls. In conclusion, 
      elevated maternal testosterone levels decrease urinary elimination of PFOA, 
      possibly through altered expression of renal transporters leading to increased 
      tissue concentrations of PFOA in pregnant rats.
FAU - Yadav, Pankaj
AU  - Yadav P
AD  - Department of Comparative Biosciences, School of Veterinary Medicine, University 
      of Wisconsin, Madison, Wisconsin, United States of America.
FAU - Mishra, Jay S
AU  - Mishra JS
AD  - Department of Comparative Biosciences, School of Veterinary Medicine, University 
      of Wisconsin, Madison, Wisconsin, United States of America.
FAU - Kumar, Sathish
AU  - Kumar S
AD  - Department of Comparative Biosciences, School of Veterinary Medicine, University 
      of Wisconsin, Madison, Wisconsin, United States of America.
AD  - Department of Obstetrics and Gynecology, School of Medicine and Public Health, 
      University of Wisconsin, Madison, Wisconsin, United States of America.
AD  - Endocrinology-Reproductive Physiology Program, University of Wisconsin, Madison, 
      Wisconsin, United States of America.
LA  - eng
GR  - R01 ES033345/ES/NIEHS NIH HHS/United States
GR  - R01 HL134779/HL/NHLBI NIH HHS/United States
PT  - Journal Article
DEP - 20230803
PL  - United States
TA  - J Environ Sci Public Health
JT  - Journal of environmental science and public health
JID - 9918626489006676
PMC - PMC10470210
MID - NIHMS1923912
OTO - NOTNLM
OT  - Bioaccumulation
OT  - Kidney
OT  - Perfluorooctanoic acid
OT  - Pregnant rats
OT  - Testosterone
OT  - Transporters
COIS- Conflicts of interest: None
EDAT- 2023/09/01 06:43
MHDA- 2023/09/01 06:44
PMCR- 2023/08/31
CRDT- 2023/09/01 04:04
PHST- 2023/09/01 06:44 [medline]
PHST- 2023/09/01 06:43 [pubmed]
PHST- 2023/09/01 04:04 [entrez]
PHST- 2023/08/31 00:00 [pmc-release]
AID - 10.26502/jesph.96120193 [doi]
PST - ppublish
SO  - J Environ Sci Public Health. 2023;7(3):131-139. doi: 10.26502/jesph.96120193. 
      Epub 2023 Aug 3.