PMID- 37655721
OWN - NLM
STAT- MEDLINE
DCOM- 20231225
LR  - 20240109
IS  - 2050-7518 (Electronic)
IS  - 2050-750X (Linking)
VI  - 12
IP  - 1
DP  - 2023 Dec 22
TI  - Mitochondria-targeting nanozyme alleviating temporomandibular joint pain by 
      inhibiting the TNFalpha/NF-kappaB/NEAT1 pathway.
PG  - 112-121
LID - 10.1039/d3tb00929g [doi]
AB  - Inflammatory cytokines that are secreted into the spinal trigeminal nucleus 
      caudalis (Sp5C) may augment inflammation and cause pain associated with 
      temporomandibular joint disorders (TMD). In a two-step process, we attached 
      triphenylphosphonium (TPP) to the surface of a cubic liposome metal-organic 
      framework (MOF) loaded with ruthenium (Ru) nanozyme. The design targeted 
      mitochondria and was designated Mito-Ru MOF. This structure scavenges free 
      radicals and reactive oxygen species (ROS) and alleviates oxidative stress. The 
      present study aimed to investigate the effects and mechanisms by which Mito-Ru 
      MOF ameliorates TMD pain. Intra-temporomandibular joint (TMJ) injections of 
      complete Freund's adjuvant (CFA) induced inflammatory pain for >/=10 d in the skin 
      areas innervated by the trigeminal nerve. Tumor necrosis factor-alpha (TNF-alpha), 
      nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappaB), long 
      non-coding RNA nuclear paraspeckle assembly transcript 1 (lncRNA NEAT1), and ROS 
      also have been proved to be significantly upregulated in the Sp5C of TMD mice. 
      Moreover, a single Mito-Ru MOF treatment alleviated TMD pain for 3 d and 
      downregulated TNF-alpha, NF-kappaB, lncRNA NEAT1, and ROS. NF-kappaB knockdown downregulated 
      NEAT1 in the TMD mice. Hence, Mito-Ru MOF inhibited the production of ROS and 
      alleviated CFA-induced TMD pain via the TNF-alpha/NF-kappaB/NEAT1 pathway. Therefore, 
      Mito-Ru MOF could effectively treat the pain related to TMD and other conditions 
      associated with severe acute inflammatory activation.
FAU - Bai, Qian
AU  - Bai Q
AD  - Medical Research Center, The Second Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, Henan, 450052, China.
FAU - Zhou, Yaoyao
AU  - Zhou Y
AD  - Medical Research Center, The Second Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, Henan, 450052, China.
FAU - Cui, Xiaona
AU  - Cui X
AD  - Medical Research Center, The Second Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, Henan, 450052, China.
AD  - Department of Critical Care Medicine, The Second Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, Henan, China. liuxiaojunzzu@163.com.
FAU - Si, Haichao
AU  - Si H
AD  - Department of Anesthesiology, Nanyang Central Hospital, Nanyang, Henan, China.
FAU - Wu, Tingting
AU  - Wu T
AD  - Medical Research Center, The Second Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, Henan, 450052, China.
FAU - Nasir, Abdul
AU  - Nasir A
AD  - Medical Research Center, The Second Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, Henan, 450052, China.
AD  - Department of Anesthesiology and Perioperative Medicine, The Second Affiliated 
      Hospital of Zhengzhou University, Zhengzhou, Henan, China.
FAU - Ma, Heng
AU  - Ma H
AD  - Medical Research Center, The Second Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, Henan, 450052, China.
AD  - Department of Anesthesiology and Perioperative Medicine, The Second Affiliated 
      Hospital of Zhengzhou University, Zhengzhou, Henan, China.
FAU - Xing, Junyue
AU  - Xing J
AD  - National Health Commission Key Laboratory of Cardiovascular Regenerative 
      Medicine, Heart Center of Henan Provincial People's Hospital, Central China Fuwai 
      Hospital of Zhengzhou University, Fuwai Central China Cardiovascular Hospital & 
      Central China Branch of National Center for Cardiovascular Diseases, Zhengzhou, 
      Henan, 451464, China. tangpku_zzuhao@zzu.edu.cn.
FAU - Wang, Yingying
AU  - Wang Y
AD  - National Health Commission Key Laboratory of Cardiovascular Regenerative 
      Medicine, Heart Center of Henan Provincial People's Hospital, Central China Fuwai 
      Hospital of Zhengzhou University, Fuwai Central China Cardiovascular Hospital & 
      Central China Branch of National Center for Cardiovascular Diseases, Zhengzhou, 
      Henan, 451464, China. tangpku_zzuhao@zzu.edu.cn.
FAU - Cheng, Xiaolei
AU  - Cheng X
AD  - National Health Commission Key Laboratory of Cardiovascular Regenerative 
      Medicine, Heart Center of Henan Provincial People's Hospital, Central China Fuwai 
      Hospital of Zhengzhou University, Fuwai Central China Cardiovascular Hospital & 
      Central China Branch of National Center for Cardiovascular Diseases, Zhengzhou, 
      Henan, 451464, China. tangpku_zzuhao@zzu.edu.cn.
FAU - Liu, Xiaojun
AU  - Liu X
AD  - Department of Critical Care Medicine, The Second Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, Henan, China. liuxiaojunzzu@163.com.
FAU - Qi, Shaoyan
AU  - Qi S
AD  - Department of Critical Care Medicine, The Second Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, Henan, China. liuxiaojunzzu@163.com.
FAU - Li, Zhisong
AU  - Li Z
AD  - Department of Anesthesiology and Perioperative Medicine, The Second Affiliated 
      Hospital of Zhengzhou University, Zhengzhou, Henan, China.
FAU - Tang, Hao
AU  - Tang H
AUID- ORCID: 0000-0001-5401-7295
AD  - National Health Commission Key Laboratory of Cardiovascular Regenerative 
      Medicine, Heart Center of Henan Provincial People's Hospital, Central China Fuwai 
      Hospital of Zhengzhou University, Fuwai Central China Cardiovascular Hospital & 
      Central China Branch of National Center for Cardiovascular Diseases, Zhengzhou, 
      Henan, 451464, China. tangpku_zzuhao@zzu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231222
PL  - England
TA  - J Mater Chem B
JT  - Journal of materials chemistry. B
JID - 101598493
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (triphenylphosphonium)
SB  - IM
EIN - J Mater Chem B. 2023 Dec 6;:. PMID: 38054383
MH  - Mice
MH  - Animals
MH  - *NF-kappa B/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - Pain/metabolism/pathology
MH  - Temporomandibular Joint/metabolism/pathology
EDAT- 2023/09/01 12:43
MHDA- 2023/12/25 06:41
CRDT- 2023/09/01 07:14
PHST- 2023/12/25 06:41 [medline]
PHST- 2023/09/01 12:43 [pubmed]
PHST- 2023/09/01 07:14 [entrez]
AID - 10.1039/d3tb00929g [doi]
PST - epublish
SO  - J Mater Chem B. 2023 Dec 22;12(1):112-121. doi: 10.1039/d3tb00929g.