PMID- 37656330
OWN - NLM
STAT- Publisher
LR  - 20230901
IS  - 1573-4927 (Electronic)
IS  - 0006-2928 (Linking)
DP  - 2023 Sep 1
TI  - MiR-5189-3p Suppresses cell Proliferation, Invasion and Migration Through 
      Targeting EIF5A2 in Laryngeal Squamous Cell Carcinoma.
LID - 10.1007/s10528-023-10489-4 [doi]
AB  - A growing body of evidence suggests that miR-5189-3p plays a critical role in 
      multiple diseases. This study aimed to investigate the function of miR-5189-3p in 
      laryngeal squamous cell carcinoma (LSCC) and explore its underlying mechanisms. 
      qRT-PCR was designed to determine the expression levels of miR-5189-3p and 
      eukaryotic translation initiation factor 5A2 (EIF5A2), while CCK-8 assay was 
      performed to measure the effects of miR-5189-3p on cell proliferation. Transwell 
      assay was performed to evaluate cell invasion as well as migration, and wound 
      healing assay was applied to demonstrate cell migratory ability. Target gene 
      prediction and luciferase reporter assay were developed to screen the possible 
      target gene of miR-5189-3p, and Western blot was designed to measure EIF5A2 
      protein expression. MiR-5189-3p was down-regulated in LSCC tissues and cell 
      lines. Up-regulation of miR-5189-3p notably inhibited cell proliferation, 
      invasion, and migration in HEP2 and FADU cells. EIF5A2 was the potential 
      downstream gene of miR-5189-3p, and overexpression of miR-5189-3p apparently 
      reduced EIF5A2 expression. Moreover, reintroduction of EIF5A2 rescued the tumor 
      suppressive effects of miR-5189-3p. MiR-5189-3p functions as a tumor inhibitor in 
      LSCC progression via directly regulating EIF5A2 and may be a potential 
      therapeutic target for LSCC.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Lou, Dan
AU  - Lou D
AD  - Department of Otolaryngology, The Second Hospital of Hebei Medical University, 
      No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.
AD  - Department of Otolaryngology, The First Hospital of Qinhuangdao, Qinhuangdao, 
      066000, Hebei, China.
FAU - Jia, Qiaojing
AU  - Jia Q
AD  - Department of Otolaryngology, The Second Hospital of Hebei Medical University, 
      No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.
FAU - Zhang, Haizhong
AU  - Zhang H
AD  - Department of Otolaryngology, The Second Hospital of Hebei Medical University, 
      No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.
FAU - Wang, Jingmiao
AU  - Wang J
AD  - Department of Otolaryngology, The Second Hospital of Hebei Medical University, 
      No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.
FAU - Liu, Lisha
AU  - Liu L
AD  - Department of Otolaryngology, The Second Hospital of Hebei Medical University, 
      No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.
FAU - Liu, Zhichang
AU  - Liu Z
AD  - Department of Otolaryngology, The Second Hospital of Hebei Medical University, 
      No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.
FAU - Jia, Xiaofang
AU  - Jia X
AD  - Department of Otolaryngology, The Second Hospital of Hebei Medical University, 
      No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.
FAU - Wang, Jianxing
AU  - Wang J
AD  - Department of Otolaryngology, The Second Hospital of Hebei Medical University, 
      No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China. 
      jianxingwang2008@163.com.
FAU - Shan, Chunguang
AU  - Shan C
AD  - Department of Otolaryngology, The Second Hospital of Hebei Medical University, 
      No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China. 
      shanchunguang0825@hb2h.com.
LA  - eng
GR  - 20372401D/Hebei Province Development of Bureau Science and Technology/
PT  - Journal Article
DEP - 20230901
PL  - United States
TA  - Biochem Genet
JT  - Biochemical genetics
JID - 0126611
SB  - IM
OTO - NOTNLM
OT  - EIF5A2
OT  - Laryngeal carcinoma
OT  - Target
OT  - miR-5189-3p
EDAT- 2023/09/01 12:43
MHDA- 2023/09/01 12:43
CRDT- 2023/09/01 11:13
PHST- 2023/04/23 00:00 [received]
PHST- 2023/08/06 00:00 [accepted]
PHST- 2023/09/01 12:43 [medline]
PHST- 2023/09/01 12:43 [pubmed]
PHST- 2023/09/01 11:13 [entrez]
AID - 10.1007/s10528-023-10489-4 [pii]
AID - 10.1007/s10528-023-10489-4 [doi]
PST - aheadofprint
SO  - Biochem Genet. 2023 Sep 1. doi: 10.1007/s10528-023-10489-4.