PMID- 37659636
OWN - NLM
STAT- Publisher
LR  - 20231022
IS  - 1096-0953 (Electronic)
IS  - 0013-9351 (Linking)
VI  - 237
IP  - Pt 2
DP  - 2023 Nov 15
TI  - Environmental exposure to chlorpyrifos during gestation, APOE polymorphism and 
      the risk on autistic-like behaviors.
PG  - 116969
LID - S0013-9351(23)01773-5 [pii]
LID - 10.1016/j.envres.2023.116969 [doi]
AB  - Autism spectrum disorder (ASD) encompasses several neurodevelopmental conditions 
      characterized by communication and social impairment, as well as repetitive 
      patterns of behavior. However, it can co-occur with other mental conditions such 
      as anxiety. The massive use of chlorpyrifos (CPF) has been linked to the 
      increased prevalence of developmental disorders. Likewise, ASD has also been 
      closely linked to a wide variety of genetic factors. The aims of the present 
      investigation are to study how gestational CPF exposure and APOE polymorphism 
      affects communication skills, early development and mid-term anxiety-like 
      behaviors, as well as, changes in gene expression related to the cholinergic 
      system. C57BL/6J and humanized apoE3 and apoE4 homozygous mice were exposed to 0 
      or 1 mg/kg/day of CPF through the diet, from gestational day (GD) 12-18. In 
      addition, a group of C57BL/6J females were injected subcutaneously with 
      300 mg/kg/day of valproic acid (VPA) on GD 12 and 13. This group was used as a 
      positive control for studying some core and associated autism-like behaviors. 
      Communication skills by means of ultrasonic vocalizations and physical/motor 
      development were assessed during the preweaning period, whereas locomotor 
      activity, anxiety-like behaviors and the gene expression of cholinergic elements 
      were evaluated during adolescence. Our results showed that C57BL/6J mice 
      prenatally exposed to CPF or VPA showed a decrease in body weight and a delay in 
      eye opening. Communication and anxiety behavior were affected differently 
      depending on treatment, while gene expression was altered by sex and treatment. 
      In addition, none of the parameters evaluated in apoE transgenic mice exposed to 
      CPF were affected, but there were differences between genotypes. Therefore, we 
      suggest that prenatal CPF exposure and VPA produce divergent effects on 
      communication and anxiety.
CI  - Copyright (c) 2023 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Biosca-Brull, Judit
AU  - Biosca-Brull J
AD  - Universitat Rovira i Virgili, Research Group in Neurobehavior and Health 
      (NEUROLAB), Tarragona, Spain; Universitat Rovira i Virgili, Department of 
      Psychology and Research Center for Behavior Assessment (CRAMC), Tarragona, Spain; 
      Universitat Rovira i Virgili, Center of Environmental, Food and Toxicological 
      Technology (TECNATOX), Reus, Spain. Electronic address: judit.biosca@urv.cat.
FAU - Basaure, Pia
AU  - Basaure P
AD  - Universitat Rovira i Virgili, Research Group in Neurobehavior and Health 
      (NEUROLAB), Tarragona, Spain.
FAU - Guardia-Escote, Laia
AU  - Guardia-Escote L
AD  - Universitat Rovira i Virgili, Research Group in Neurobehavior and Health 
      (NEUROLAB), Tarragona, Spain.
FAU - Cabre, Maria
AU  - Cabre M
AD  - Universitat Rovira i Virgili, Research Group in Neurobehavior and Health 
      (NEUROLAB), Tarragona, Spain; Universitat Rovira i Virgili, Department of 
      Biochemistry and Biotechnology, Tarragona, Spain.
FAU - Blanco, Jordi
AU  - Blanco J
AD  - Universitat Rovira i Virgili, Research Group in Neurobehavior and Health 
      (NEUROLAB), Tarragona, Spain; Universitat Rovira i Virgili, Center of 
      Environmental, Food and Toxicological Technology (TECNATOX), Reus, Spain; 
      Universitat Rovira i Virgili, Department of Basic Medical Sciences, Reus, Spain.
FAU - Morales-Navas, Miguel
AU  - Morales-Navas M
AD  - Department of Psychology, Health Research Center (CEINSA), Almeria University, 
      04120, Almeria, Spain.
FAU - Sanchez-Santed, Fernando
AU  - Sanchez-Santed F
AD  - Department of Psychology, Health Research Center (CEINSA), Almeria University, 
      04120, Almeria, Spain.
FAU - Colomina, Maria Teresa
AU  - Colomina MT
AD  - Universitat Rovira i Virgili, Research Group in Neurobehavior and Health 
      (NEUROLAB), Tarragona, Spain; Universitat Rovira i Virgili, Department of 
      Psychology and Research Center for Behavior Assessment (CRAMC), Tarragona, Spain; 
      Universitat Rovira i Virgili, Center of Environmental, Food and Toxicological 
      Technology (TECNATOX), Reus, Spain. Electronic address: 
      mariateresa.colomina@urv.cat.
LA  - eng
PT  - Journal Article
DEP - 20230831
PL  - Netherlands
TA  - Environ Res
JT  - Environmental research
JID - 0147621
SB  - IM
OTO - NOTNLM
OT  - APOE genotype
OT  - Anxiety
OT  - Autism
OT  - Chlorpyrifos
OT  - Cholinergic system
OT  - Development
OT  - Ultrasonic vocalizations
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/09/03 00:41
MHDA- 2023/09/03 00:41
CRDT- 2023/09/02 19:26
PHST- 2023/07/31 00:00 [received]
PHST- 2023/08/04 00:00 [revised]
PHST- 2023/08/22 00:00 [accepted]
PHST- 2023/09/03 00:41 [pubmed]
PHST- 2023/09/03 00:41 [medline]
PHST- 2023/09/02 19:26 [entrez]
AID - S0013-9351(23)01773-5 [pii]
AID - 10.1016/j.envres.2023.116969 [doi]
PST - ppublish
SO  - Environ Res. 2023 Nov 15;237(Pt 2):116969. doi: 10.1016/j.envres.2023.116969. 
      Epub 2023 Aug 31.