PMID- 37660522
OWN - NLM
STAT- MEDLINE
DCOM- 20231201
LR  - 20231217
IS  - 2300-732X (Electronic)
IS  - 1642-431X (Linking)
VI  - 23
IP  - 4
DP  - 2023 Dec
TI  - LC-MS-based untargeted metabolomics reveals the mechanism underlying prostate 
      damage in a type 2 diabetes mouse model.
PG  - 100811
LID - S1642-431X(23)00083-9 [pii]
LID - 10.1016/j.repbio.2023.100811 [doi]
AB  - Type 2 diabetes mellitus (T2DM) can cause prostate damage and affect male 
      reproductive function, but the underlying mechanisms are not completely 
      understood. In this study, we used liquid chromatography-mass spectrometry 
      (LC-MS)-based untargeted metabolomics to identify endogenous metabolites in the 
      prostate of a T2DM mouse model. The selected endogenous metabolites were then 
      subjected to bioinformatics analysis and metabolic pathway studies to understand 
      their role in the development of T2DM-induced prostate damage. We used male 
      homozygous BTBR ob/ob mice (n = 12) and BTBR WT mice (n = 11) in this study. We 
      monitored changes in blood glucose, body weight, prostate weight, and prostate 
      index, as well as performed hematoxylin and eosin (H&E) staining and observed 
      that the prostate of the BTBR ob/ob was damaged. We then used 
      ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight 
      mass spectrometry (UPLC-Q-TOF-MS) for metabolomics analysis. The stability of the 
      model was validated using principal component analysis (PCA) and orthogonal 
      partial least squares-discriminant analysis (OPLS-DA). Using variable importance 
      in projection (VIP) > 1, false discovery rate (FDR) < 0.05, and coefficient of 
      variation (CV) < 30 as criteria, a total of 149 differential metabolites (62 
      upregulated and 87 downregulated) were identified between the prostates of the 
      two groups of mice. Topological pathway analysis showed that these differential 
      metabolites were mainly involved in sphingolipid (SP) and glycerophospholipid 
      (GP) metabolism. In conclusion, our study not only emphasizes the damage caused 
      by T2DM to the prostate but also provides new insights into the potential 
      mechanisms of T2DM-induced male reproductive dysfunction.
CI  - Copyright (c) 2023 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Qi, Rong
AU  - Qi R
AD  - Clinical Medical College, North China University of Science and Technology, 
      Tangshan 063210, China.
FAU - Kang, Shao-San
AU  - Kang SS
AD  - Clinical Medical College, North China University of Science and Technology, 
      Tangshan 063210, China.
FAU - Pei, Yongchao
AU  - Pei Y
AD  - Clinical Medical College, North China University of Science and Technology, 
      Tangshan 063210, China.
FAU - Liu, Mingming
AU  - Liu M
AD  - School of Public Health, North China University of Science and Technology, 
      Tangshan 063210, China.
FAU - Zhou, Yufan
AU  - Zhou Y
AD  - School of Public Health, North China University of Science and Technology, 
      Tangshan 063210, China.
FAU - Guan, Bo
AU  - Guan B
AD  - School of Public Health, North China University of Science and Technology, 
      Tangshan 063210, China.
FAU - Zhang, Xinduo
AU  - Zhang X
AD  - School of Public Health, North China University of Science and Technology, 
      Tangshan 063210, China.
FAU - Li, Zhiguo
AU  - Li Z
AD  - School of Public Health, North China University of Science and Technology, 
      Tangshan 063210, China. Electronic address: lizhiguo@ncst.edu.cn.
FAU - Cao, Fenghong
AU  - Cao F
AD  - Clinical Medical College, North China University of Science and Technology, 
      Tangshan 063210, China. Electronic address: caofenghong@163.com.
LA  - eng
PT  - Journal Article
DEP - 20230901
PL  - Poland
TA  - Reprod Biol
JT  - Reproductive biology
JID - 101160559
RN  - 0 (Biomarkers)
SB  - IM
MH  - Male
MH  - Mice
MH  - Animals
MH  - Chromatography, Liquid/methods
MH  - *Diabetes Mellitus, Type 2
MH  - Prostate/metabolism
MH  - Tandem Mass Spectrometry
MH  - Metabolomics/methods
MH  - Disease Models, Animal
MH  - Biomarkers/metabolism
OTO - NOTNLM
OT  - Glycerophospholipid
OT  - Metabolomics
OT  - Prostate
OT  - Sphingolipid
OT  - Type 2 diabetes mellitus
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/09/04 00:41
MHDA- 2023/12/01 06:45
CRDT- 2023/09/03 18:05
PHST- 2023/05/30 00:00 [received]
PHST- 2023/08/18 00:00 [revised]
PHST- 2023/08/24 00:00 [accepted]
PHST- 2023/12/01 06:45 [medline]
PHST- 2023/09/04 00:41 [pubmed]
PHST- 2023/09/03 18:05 [entrez]
AID - S1642-431X(23)00083-9 [pii]
AID - 10.1016/j.repbio.2023.100811 [doi]
PST - ppublish
SO  - Reprod Biol. 2023 Dec;23(4):100811. doi: 10.1016/j.repbio.2023.100811. Epub 2023 
      Sep 1.