PMID- 37661044
OWN - NLM
STAT- MEDLINE
DCOM- 20230920
LR  - 20230920
IS  - 1532-0456 (Print)
IS  - 1532-0456 (Linking)
VI  - 273
DP  - 2023 Nov
TI  - Insights into mechanism of DNA damage and repair-apoptosis in digestive gland of 
      female scallop Chlamys farreri under benzo[a]pyrene exposure during reproductive 
      stage.
PG  - 109738
LID - S1532-0456(23)00193-X [pii]
LID - 10.1016/j.cbpc.2023.109738 [doi]
AB  - As one of the most carcinogenic persistent organic pollutants (POPs), 
      benzo[a]pyrene (B [a]P) brings high toxicity to marine bivalves. Digestive gland 
      is the most important metabolism-related organ of aquatic animals. This study 
      conducted the digestive gland transcriptome of Chlamys farreri under B[a]P 
      treatment at reproductive stages. And the reproductive-stage dependence 
      metabolism-DNA repair-apoptosis process of scallops under 0, 0.04, 0.4 and 4 mug/L 
      B[a]P was studied by qRT-PCR. The results demonstrated that the detoxification 
      metabolism was disturbed after ovulation except for CYP3A4. In antioxidant 
      system, antioxidant enzyme CAT and GPX, and GGT1 (one of the non-enzymatic 
      antioxidants synthesis gene) continuously served the function of antioxidant 
      defense. Three types of DNA repair were activated under B[a]P stress, however, 
      DNA strand breaks were still serious. B[a]P exposure weakened death receptor 
      pathway as well as enhanced mitochondrial pathway, surprisingly suppressing 
      apoptosis in scallops. In addition, ten indicators were screened by Spearman 
      correlation analysis. This study will provide sound theoretical basis for bivalve 
      toxicology and contribute to the biomonitoring of marine POPs pollution.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Zhou, Yueyao
AU  - Zhou Y
AD  - The Key Laboratory of Mariculture, Ministry of Education, Ocean University of 
      China, Qingdao 266003, PR China.
FAU - Xu, Ruiyi
AU  - Xu R
AD  - The Key Laboratory of Mariculture, Ministry of Education, Ocean University of 
      China, Qingdao 266003, PR China.
FAU - Gao, Zhongyuan
AU  - Gao Z
AD  - The Key Laboratory of Mariculture, Ministry of Education, Ocean University of 
      China, Qingdao 266003, PR China.
FAU - Miao, Jingjing
AU  - Miao J
AD  - The Key Laboratory of Mariculture, Ministry of Education, Ocean University of 
      China, Qingdao 266003, PR China.
FAU - Pan, Luqing
AU  - Pan L
AD  - The Key Laboratory of Mariculture, Ministry of Education, Ocean University of 
      China, Qingdao 266003, PR China. Electronic address: panlq@ouc.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20230901
PL  - United States
TA  - Comp Biochem Physiol C Toxicol Pharmacol
JT  - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
JID - 100959500
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - 0 (Antioxidants)
SB  - IM
MH  - Female
MH  - Animals
MH  - *Benzo(a)pyrene/toxicity
MH  - Antioxidants
MH  - *Pectinidae/genetics
MH  - DNA Damage
MH  - Apoptosis
OTO - NOTNLM
OT  - Apoptosis
OT  - Benzo[a]pyrene
OT  - Chlamys farreri
OT  - DNA repair
OT  - Detoxification metabolism
OT  - Oxidative stress
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/09/04 00:41
MHDA- 2023/09/20 06:42
CRDT- 2023/09/03 19:31
PHST- 2023/07/17 00:00 [received]
PHST- 2023/08/25 00:00 [revised]
PHST- 2023/08/30 00:00 [accepted]
PHST- 2023/09/20 06:42 [medline]
PHST- 2023/09/04 00:41 [pubmed]
PHST- 2023/09/03 19:31 [entrez]
AID - S1532-0456(23)00193-X [pii]
AID - 10.1016/j.cbpc.2023.109738 [doi]
PST - ppublish
SO  - Comp Biochem Physiol C Toxicol Pharmacol. 2023 Nov;273:109738. doi: 
      10.1016/j.cbpc.2023.109738. Epub 2023 Sep 1.