PMID- 37661073 OWN - NLM STAT- MEDLINE DCOM- 20230918 LR - 20230918 IS - 1096-1208 (Electronic) IS - 0882-4010 (Linking) VI - 183 DP - 2023 Oct TI - Comparison of heterologous prime-boost immunization strategies with DNA and recombinant vaccinia virus co-expressing GP3 and GP5 of European type porcine reproductive and respiratory syndrome virus in pigs. PG - 106328 LID - S0882-4010(23)00361-3 [pii] LID - 10.1016/j.micpath.2023.106328 [doi] AB - Vaccination is principally used to control and treat porcine reproductive and respiratory syndrome virus (PRRSV) infection. This study investigated immunogenicity and protective efficacy of heterologous prime-boost regimens in pigs, including recombinant DNA and vaccinia virus vectors coexpressing PRRSV European genotype (EU) isolate GP3 and GP5: group A, pVAX1-EU-GP3-GP5 prime and rddVTT-EU-GP3-GP5 boost; group B, rddVTT-EU-GP3-GP5 prime and pVAX1-EU-GP3-GP5 boost; group C, empty vector pVAX1; group D, E3L gene-deleted vaccinia virus E3L- VTT. Vaccine efficacy was tested in an EU-type PRRSV (Lelystad virus strain) challenge pig model based on evaluating PRRSV-specific antibody responses, neutralizing antibodies, cytokines, T lymphocyte proliferation, CD4(+) and CD8(+) T lymphocytes, clinical symptoms, viremia and tissue virus loads. Plasmid DNA was delivered as chitosan-DNA nanoparticles, and Quil A (Quillaja) was used to increase vaccine efficiency. All piglets were boosted 21 days post the initial inoculation (dpi) and then challenged 14 days later. At 14, 21, 28 and 35 dpi, groups A and B developed significantly higher PRRSV-specific antibody responses compared with control groups C and D. Two weeks after the boost, significant differences in neutralizing antibody and IFN-gamma levels were observed between groups A, C, D and B. At 49 dpi, groups A and B had markedly increased peripheral blood CD3(+)CD4(+) T cell levels. Following virus challenge, group A showed viremia, but organ virus loads were lower than those in other groups. Thus, a heterologous prime-boost vaccine regimen (rddVTT-EU-GP3-GP5 prime, pVAX1-EU-GP3-GP5 boost) can improve humoral- and cell-mediated immune responses to provide resistance to EU-type PRRSV infection in vivo. CI - Copyright (c) 2023 Elsevier Ltd. All rights reserved. FAU - Zhang, Hewei AU - Zhang H AD - The 989th Hospital of the Joint Logistics Support Force of Chinese People's Liberation Army, Luoyang, 471031, China; College of Food and Drugs, Luoyang Polytechnic, Luo Yang, 471000, China; Animal Diseases and Public Health Engineering Research Center of Henan Province, Luoyang, 471000, China. FAU - Ren, Jingqiang AU - Ren J AD - Institute of Virology, Wenzhou University, Chashan University Town, Wenzhou, 325035, China; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, 130122, China; Animal Diseases and Public Health Engineering Research Center of Henan Province, Luoyang, 471000, China; Key Laboratory of Special Animal Epidemic Disease, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, 130112, China. Electronic address: rjq207@163.com. FAU - Li, Jiachen AU - Li J AD - College of Food and Drugs, Luoyang Polytechnic, Luo Yang, 471000, China; Animal Diseases and Public Health Engineering Research Center of Henan Province, Luoyang, 471000, China. FAU - Zhai, Chongkai AU - Zhai C AD - College of Food and Drugs, Luoyang Polytechnic, Luo Yang, 471000, China; Animal Diseases and Public Health Engineering Research Center of Henan Province, Luoyang, 471000, China. FAU - Mao, Fuchao AU - Mao F AD - College of Food and Drugs, Luoyang Polytechnic, Luo Yang, 471000, China; Animal Diseases and Public Health Engineering Research Center of Henan Province, Luoyang, 471000, China. FAU - Yang, Shaozhe AU - Yang S AD - Animal Diseases and Public Health Engineering Research Center of Henan Province, Luoyang, 471000, China. FAU - Zhang, Qingwei AU - Zhang Q AD - Animal Diseases and Public Health Engineering Research Center of Henan Province, Luoyang, 471000, China. FAU - Liu, Zhongyu AU - Liu Z AD - The 989th Hospital of the Joint Logistics Support Force of Chinese People's Liberation Army, Luoyang, 471031, China; College of Food and Drugs, Luoyang Polytechnic, Luo Yang, 471000, China; Animal Diseases and Public Health Engineering Research Center of Henan Province, Luoyang, 471000, China. Electronic address: liuzhongyu@lypt.edu.cn. FAU - Fu, Xiuhong AU - Fu X AD - Animal Diseases and Public Health Engineering Research Center of Henan Province, Luoyang, 471000, China. Electronic address: fsh0430@sina.com. LA - eng PT - Journal Article DEP - 20230901 PL - England TA - Microb Pathog JT - Microbial pathogenesis JID - 8606191 RN - 9007-49-2 (DNA) RN - 0 (Antibodies, Neutralizing) RN - 0 (Antibodies, Viral) SB - IM MH - Animals MH - Swine MH - *Porcine respiratory and reproductive syndrome virus/genetics MH - Vaccinia virus/genetics MH - Viremia/prevention & control MH - Vaccination MH - Immunization MH - DNA MH - Antibodies, Neutralizing MH - Antibodies, Viral OTO - NOTNLM OT - Adjuvant OT - DNA vaccine OT - Heterologous prime-boost OT - Porcine reproductive and respiratory syndrome virus OT - Vaccinia virus vaccine COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper EDAT- 2023/09/04 00:41 MHDA- 2023/09/18 12:43 CRDT- 2023/09/03 19:32 PHST- 2023/04/07 00:00 [received] PHST- 2023/08/27 00:00 [revised] PHST- 2023/08/28 00:00 [accepted] PHST- 2023/09/18 12:43 [medline] PHST- 2023/09/04 00:41 [pubmed] PHST- 2023/09/03 19:32 [entrez] AID - S0882-4010(23)00361-3 [pii] AID - 10.1016/j.micpath.2023.106328 [doi] PST - ppublish SO - Microb Pathog. 2023 Oct;183:106328. doi: 10.1016/j.micpath.2023.106328. Epub 2023 Sep 1.