PMID- 37662685
OWN - NLM
STAT- MEDLINE
DCOM- 20230905
LR  - 20230905
IS  - 1178-2013 (Electronic)
IS  - 1176-9114 (Print)
IS  - 1176-9114 (Linking)
VI  - 18
DP  - 2023
TI  - Influence of GO-Antisense miRNA-21 on the Expression of Selected Cytokines at 
      Glioblastoma Cell Lines.
PG  - 4839-4855
LID - 10.2147/IJN.S419957 [doi]
AB  - INTRODUCTION: Graphene oxide (GO) is a single layer of carbon atoms with unique 
      properties, which are beneficial due to its surface functionalisation by miRNA. 
      miRNAs are a non-coding small form of RNA that suppress the expression of 
      protein-coding genes by translational repression or degradation of messenger RNA. 
      Antisense miRNA-21 is very promising for future investigation in cancer therapy. 
      This study aimed to detect cytokine expression levels after the administration of 
      GO-antisense miRNA-21 into U87, U118, U251 and T98 glioma cell lines. METHODS: 
      U87, U118, U251 and T98 glioma cell line were investigated in term of viability, 
      human cytokine expression level at protein and genes after treatment with GO, 
      GO-antisense miRNA-21 and antisense miRNA-21. The delivery of antisense miRNA-21 
      into the glioma cell at in vitro investigation were conducted by GO based 
      transfection and electroporation. RESULTS: The results of the protein microarray 
      and gene expression profile showed that complexes of GO-antisense miRNA-21 
      modified the metallopeptidase inhibitor 2 (TIMP-2), interleukin-6 (IL-6), 
      interleukin 8 (IL-8), intercellular adhesion molecule 1 (ICAM-1), and monocyte 
      chemoattractant protein-1 (MCP-1) expression level compared to transfection by 
      electroporation of antisense miRNA-21 at investigated glioblastoma cell lines. 
      The TIMP-2 protein and gene expression level was upregulated after antisense 
      miRNA-21 delivery by GO complex into U87, U251 and T98 glioblastoma cell lines 
      comparing to the non-treated control group. The downregulation at protein 
      expression level of ICAM - 1 was observed at U87, U118, U251 and T98 glioma cell 
      lines. Moreover, the IL-8 expression level at mRNA for genes and protein was 
      decreased significantly after delivery the antisense-miRNA-21 by GO compared to 
      electroporation as a transfection method. DISCUSSION: This work demonstrated that 
      the graphene oxide complexes with antisense miRNA-21 can effectively modulate the 
      cytokine mRNA and protein expression level at U87, U118, U251 and T98 glioma cell 
      lines.
CI  - (c) 2023 Kutwin et al.
FAU - Kutwin, Marta
AU  - Kutwin M
AD  - Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life 
      Sciences, Warsaw, 02-786, Poland.
FAU - Sosnowska, Malwina
AU  - Sosnowska M
AUID- ORCID: 0000-0002-1882-0846
AD  - Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life 
      Sciences, Warsaw, 02-786, Poland.
FAU - Ostrowska, Agnieszka
AU  - Ostrowska A
AUID- ORCID: 0000-0001-8781-6106
AD  - Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life 
      Sciences, Warsaw, 02-786, Poland.
FAU - Trzaskowski, Maciej
AU  - Trzaskowski M
AUID- ORCID: 0000-0001-5892-1898
AD  - Centre for Advanced Materials and Technologies CEZAMAT, Warsaw University of 
      Technology, Warsaw, 02-822, Poland.
FAU - Lange, Agata
AU  - Lange A
AUID- ORCID: 0000-0003-1523-083X
AD  - Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life 
      Sciences, Warsaw, 02-786, Poland.
FAU - Wierzbicki, Mateusz
AU  - Wierzbicki M
AUID- ORCID: 0000-0003-3623-8929
AD  - Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life 
      Sciences, Warsaw, 02-786, Poland.
FAU - Jaworski, Slawomir
AU  - Jaworski S
AUID- ORCID: 0000-0002-4619-941X
AD  - Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life 
      Sciences, Warsaw, 02-786, Poland.
LA  - eng
PT  - Journal Article
DEP - 20230828
PL  - New Zealand
TA  - Int J Nanomedicine
JT  - International journal of nanomedicine
JID - 101263847
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-8)
RN  - 0 (graphene oxide)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - 0 (MIRN21 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Humans
MH  - Cytokines/genetics
MH  - *Glioblastoma/genetics/therapy
MH  - Interleukin-8/genetics
MH  - Tissue Inhibitor of Metalloproteinase-2
MH  - *Glioma
MH  - Cell Line
MH  - *MicroRNAs/genetics
PMC - PMC10473248
OTO - NOTNLM
OT  - cancer
OT  - glioblastoma
OT  - graphene oxide
OT  - miRNA delivery
OT  - miRNA-21
COIS- The authors report no conflicts of interest in this work.
EDAT- 2023/09/04 06:42
MHDA- 2023/09/05 06:42
PMCR- 2023/08/28
CRDT- 2023/09/04 04:47
PHST- 2023/05/04 00:00 [received]
PHST- 2023/07/24 00:00 [accepted]
PHST- 2023/09/05 06:42 [medline]
PHST- 2023/09/04 06:42 [pubmed]
PHST- 2023/09/04 04:47 [entrez]
PHST- 2023/08/28 00:00 [pmc-release]
AID - 419957 [pii]
AID - 10.2147/IJN.S419957 [doi]
PST - epublish
SO  - Int J Nanomedicine. 2023 Aug 28;18:4839-4855. doi: 10.2147/IJN.S419957. 
      eCollection 2023.