PMID- 37664596
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230905
IS  - 2589-0042 (Electronic)
IS  - 2589-0042 (Linking)
VI  - 26
IP  - 9
DP  - 2023 Sep 15
TI  - In vivo spontaneous activity and coital-evoked inhibition of mouse accessory 
      olfactory bulb output neurons.
PG  - 107545
LID - 10.1016/j.isci.2023.107545 [doi]
LID - 107545
AB  - Little is known about estrous effects on brain microcircuits. We examined the 
      accessory olfactory bulb (AOB) in vivo, in anesthetized naturally cycling 
      females, as model microcircuit receiving coital somatosensory information. 
      Whole-cell recordings demonstrate that output neurons are relatively 
      hyperpolarized in estrus and unexpectedly fire high frequency bursts of action 
      potentials. To mimic coitus, a calibrated artificial vagino-cervical stimulation 
      (aVCS) protocol was devised. aVCS evoked stimulus-locked local field responses in 
      the interneuron layer independent of estrous stage. The response is sensitive to 
      alpha1-adrenergic receptor blockade, as expected since aVCS increases norepinephrine 
      release in AOB. Intriguingly, only in estrus does aVCS inhibit AOB spike output. 
      Estrus-specific output reduction coincides with prolonged aVCS activation of 
      inhibitory interneurons. Accordingly, in estrus the AOB microcircuit sets the 
      stage for coital stimulation to inhibit the output neurons, possibly via high 
      frequency bursting-dependent enhancement of reciprocal synapse efficacy between 
      inter- and output neurons.
CI  - (c) 2023 The Author(s).
FAU - Lorenzon, Paolo
AU  - Lorenzon P
AD  - Department of Integrative Medical Biology, Umea University, SE90187 Umea, Sweden.
FAU - Antos, Kamil
AU  - Antos K
AD  - Department of Integrative Medical Biology, Umea University, SE90187 Umea, Sweden.
FAU - Tripathi, Anushree
AU  - Tripathi A
AD  - Department of Integrative Medical Biology, Umea University, SE90187 Umea, Sweden.
FAU - Vedin, Viktoria
AU  - Vedin V
AD  - Department of Molecular Biology, Umea University, SE90187 Umea, Sweden.
FAU - Berghard, Anna
AU  - Berghard A
AD  - Department of Molecular Biology, Umea University, SE90187 Umea, Sweden.
FAU - Medini, Paolo
AU  - Medini P
AD  - Department of Integrative Medical Biology, Umea University, SE90187 Umea, Sweden.
LA  - eng
PT  - Journal Article
DEP - 20230807
PL  - United States
TA  - iScience
JT  - iScience
JID - 101724038
PMC - PMC10470370
OTO - NOTNLM
OT  - Behavioral neuroscience
OT  - Cell biology
OT  - Neuroscience
OT  - Sensory neuroscience
COIS- The authors declare no competing interests.
EDAT- 2023/09/04 06:43
MHDA- 2023/09/04 06:44
PMCR- 2023/08/07
CRDT- 2023/09/04 05:16
PHST- 2023/03/30 00:00 [received]
PHST- 2023/04/11 00:00 [revised]
PHST- 2023/08/01 00:00 [accepted]
PHST- 2023/09/04 06:44 [medline]
PHST- 2023/09/04 06:43 [pubmed]
PHST- 2023/09/04 05:16 [entrez]
PHST- 2023/08/07 00:00 [pmc-release]
AID - S2589-0042(23)01622-X [pii]
AID - 107545 [pii]
AID - 10.1016/j.isci.2023.107545 [doi]
PST - epublish
SO  - iScience. 2023 Aug 7;26(9):107545. doi: 10.1016/j.isci.2023.107545. eCollection 
      2023 Sep 15.