PMID- 37666596
OWN - NLM
STAT- MEDLINE
DCOM- 20230906
LR  - 20230906
IS  - 1095-9939 (Electronic)
IS  - 0048-3575 (Linking)
VI  - 195
DP  - 2023 Sep
TI  - FDP-Na-induced enhancement of glycolysis impacts larval growth and development 
      and chitin biosynthesis in fall webworm, Hyphantria cunea (Lepidoptera: 
      Arctiidae).
PG  - 105560
LID - S0048-3575(23)00225-0 [pii]
LID - 10.1016/j.pestbp.2023.105560 [doi]
AB  - Fructose 1, 6-diphosphate (FDP) is an endogenous intermediate in the glycolytic 
      pathway, as well as an allosteric activator of phosphofructokinase (PFK). Based 
      on the role in promoting glycolysis, FDP has been widely used as a therapeutic 
      agent for mitigating the damage of endotoxemia and ischemia/reperfusion in 
      clinical practice. However, the effect of exogenous FDP-induced glycolysis 
      activation on insect carbohydrate metabolism and chitin synthesis remains largely 
      unclear. Here, we investigated for the first time the effects of FDP-Na, an 
      allosteric activator of PFK, on the growth and development of Hyphantria cunea 
      larvae, a serious defoliator in agriculture and forestry, especially on 
      glycolysis and chitin synthesis. The results showed that FDP-Na significantly 
      restrained the growth and development of H. cunea larvae and resulted in larval 
      lethality. After treatment with FDP-Na, hexokinase (HK), phosphofructokinase 
      (PFK) and pyruvate kinase (PK) were significantly activated, and HcHK2, HcPFK, 
      HcPK were dramatically upregulated, which suggested that FDP-Na enhanced 
      glycolysis in H. cunea larvae. Meanwhile, FDP-Na also distinctly impacted chitin 
      biosynthesis by disturbing transcriptions of genes in the chitin synthesis 
      pathway, resulting in changes of chitin contents in the midgut and epidermis of 
      H. cunea larvae. Therefore, we considered that FDP-Na caused the growth and 
      development arrest, and impacted chitin biosynthesis, probably by disturbing in 
      vivo glycolysis and carbohydrate metabolism in H. cunea larvae. The findings 
      provide a new perspective on the mechanism by which glycolysis regulates insect 
      growth and development, and lay the foundation for exploring the potential 
      application of glycolysis activators in pest control as well.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Zhang, Shengyu
AU  - Zhang S
AD  - School of Forestry, Northeast Forestry University, Harbin 150040, PR China.
FAU - Zhang, Yu
AU  - Zhang Y
AD  - School of Forestry, Northeast Forestry University, Harbin 150040, PR China.
FAU - Zou, Haifeng
AU  - Zou H
AD  - School of Forestry, Northeast Forestry University, Harbin 150040, PR China.
FAU - Li, Xingpeng
AU  - Li X
AD  - Jilin Agricultural University, Jilin 132013, PR China.
FAU - Zou, Hang
AU  - Zou H
AD  - School of Forestry, Northeast Forestry University, Harbin 150040, PR China.
FAU - Wang, Ze
AU  - Wang Z
AD  - School of Forestry, Northeast Forestry University, Harbin 150040, PR China.
FAU - Zou, Chuanshan
AU  - Zou C
AD  - School of Forestry, Northeast Forestry University, Harbin 150040, PR China. 
      Electronic address: zoucs1980@126.com.
LA  - eng
PT  - Journal Article
DEP - 20230727
PL  - United States
TA  - Pestic Biochem Physiol
JT  - Pesticide biochemistry and physiology
JID - 1301573
RN  - 0 (Diphosphates)
RN  - 18389-49-6 (formycin diphosphate)
RN  - EC 2.7.1 - (Phosphofructokinases)
RN  - 1398-61-4 (Chitin)
SB  - IM
MH  - Animals
MH  - Larva
MH  - *Diphosphates
MH  - Glycolysis
MH  - Phosphofructokinases
MH  - Chitin/pharmacology
MH  - *Moths
OTO - NOTNLM
OT  - Chitin biosynthesis
OT  - FDP-Na
OT  - Glycolysis enhancement
OT  - Growth and development
OT  - Hyphantria cunea
COIS- Declaration of Competing Interest None.
EDAT- 2023/09/05 00:41
MHDA- 2023/09/06 06:42
CRDT- 2023/09/04 20:56
PHST- 2023/03/12 00:00 [received]
PHST- 2023/07/26 00:00 [revised]
PHST- 2023/07/26 00:00 [accepted]
PHST- 2023/09/06 06:42 [medline]
PHST- 2023/09/05 00:41 [pubmed]
PHST- 2023/09/04 20:56 [entrez]
AID - S0048-3575(23)00225-0 [pii]
AID - 10.1016/j.pestbp.2023.105560 [doi]
PST - ppublish
SO  - Pestic Biochem Physiol. 2023 Sep;195:105560. doi: 10.1016/j.pestbp.2023.105560. 
      Epub 2023 Jul 27.