PMID- 37671699
OWN - NLM
STAT- MEDLINE
DCOM- 20231124
LR  - 20231204
IS  - 1746-076X (Electronic)
IS  - 1746-0751 (Linking)
VI  - 18
IP  - 11
DP  - 2023 Nov
TI  - Secretome profile of TNF-alpha-induced human umbilical cord mesenchymal stem cells 
      unveils biological processes relevant to skin wound healing.
PG  - 839-856
LID - 10.2217/rme-2023-0085 [doi]
AB  - Aim: To profile and study the proteins responsible for the beneficial effect of 
      the TNF-alpha-induced human umbilical cord mesenchymal stem cells (hUCMSCs) secretome 
      in wound healing. Methods: The hUCMSCs secretome was generated with (induced) or 
      without (uninduced) TNF-alpha and was subsequently analyzed by liquid 
      chromatography-mass spectrometry, immunoassay and in vitro scratch assay. 
      Results: Proteomic analysis revealed approximately 260 proteins, including 51 and 
      55 unique proteins in the induced and uninduced secretomes, respectively. Gene 
      ontology analysis disclosed that differential proteins in the induced secretome 
      mainly involved inflammation-related terms. The induced secretome, consisting of 
      higher levels of FGFb, VEGF, PDGF and IL-6, significantly accelerated wound 
      closure and enhanced MMP-13 secretion in HaCaT keratinocytes. Conclusion: The 
      secretome from induced hUCMSCs includes factors that promote wound closure.
FAU - Tai, Lihui
AU  - Tai L
AUID- ORCID: 0009-0007-6141-6084
AD  - Cytopeutics Sdn Bhd, Suite 2-3 2nd floor, Bio-X Centre, Persiaran Cyberpoint 
      Selatan, Cyber 8, 63000, Cyberjaya, Selangor, Malaysia.
FAU - Saffery, Nik Syazana
AU  - Saffery NS
AUID- ORCID: 0009-0002-8860-2681
AD  - Cytopeutics Sdn Bhd, Suite 2-3 2nd floor, Bio-X Centre, Persiaran Cyberpoint 
      Selatan, Cyber 8, 63000, Cyberjaya, Selangor, Malaysia.
FAU - Chin, Sze Piaw
AU  - Chin SP
AUID- ORCID: 0000-0003-2386-7435
AD  - Cytopeutics Sdn Bhd, Suite 2-3 2nd floor, Bio-X Centre, Persiaran Cyberpoint 
      Selatan, Cyber 8, 63000, Cyberjaya, Selangor, Malaysia.
FAU - Cheong, Soon Keng
AU  - Cheong SK
AUID- ORCID: 0000-0002-3507-6713
AD  - Cytopeutics Sdn Bhd, Suite 2-3 2nd floor, Bio-X Centre, Persiaran Cyberpoint 
      Selatan, Cyber 8, 63000, Cyberjaya, Selangor, Malaysia.
AD  - M. Kandiah Faculty of Medicine & Health Sciences (MK FMHS), Universiti Tunku 
      Abdul Rahman Sungai Long City Campus, Jalan Sungai Long, Bandar Sungai Long, 
      Cheras, 43000, Kajang, Selangor, Malaysia.
LA  - eng
PT  - Journal Article
DEP - 20230906
PL  - England
TA  - Regen Med
JT  - Regenerative medicine
JID - 101278116
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Humans
MH  - *Tumor Necrosis Factor-alpha/metabolism
MH  - Secretome
MH  - Proteomics
MH  - Wound Healing
MH  - *Mesenchymal Stem Cells/metabolism
MH  - Umbilical Cord/metabolism
OAB - An interference or delay in normal stages of the wound healing process, 
      particularly in the elderly population and individuals with comorbid conditions, 
      generally results in the development of chronic wounds with uncontrolled 
      inflammation. Innovative therapies, such as stem cells and their secreted factors 
      (the 'secretome') are potential tools in regulating wound repair. We used an 
      inflammatory factor to precondition human umbilical cord stem cells to generate a 
      secretome (induced secretome) that was beneficial in response to the inflammation 
      environment. Approximately 260 proteins were detected. Further analysis 
      identified that unique proteins in the induced secretome are mainly related to 
      inflammation-related biological processes. We also demonstrated that the induced 
      secretome enhanced the wound closure rate in human keratinocyte cells, as 
      compared with the control and naive secretome. This is likely due to the higher 
      levels of growth factors and cytokines in the induced secretome, which play 
      significant roles in the regulation of the wound healing process. The present 
      findings provide useful information to better understand the role of the human 
      umbilical cord mesenchymal stem cell secretome, especially in an inflammatory 
      niche, as well as the proteins that are important for clinical translation in 
      wound repair.
OABL- eng
OTO - NOTNLM
OT  - TNF-alpha
OT  - protein profiling
OT  - secretome
OT  - umbilical cord-derived MSCs
OT  - wound healing
EDAT- 2023/09/06 12:42
MHDA- 2023/11/24 06:43
CRDT- 2023/09/06 06:53
PHST- 2023/11/24 06:43 [medline]
PHST- 2023/09/06 12:42 [pubmed]
PHST- 2023/09/06 06:53 [entrez]
AID - 10.2217/rme-2023-0085 [doi]
PST - ppublish
SO  - Regen Med. 2023 Nov;18(11):839-856. doi: 10.2217/rme-2023-0085. Epub 2023 Sep 6.