PMID- 37671855
OWN - NLM
STAT- MEDLINE
DCOM- 20240125
LR  - 20240201
IS  - 1742-8149 (Electronic)
IS  - 1464-7273 (Linking)
VI  - 26
IP  - 6
DP  - 2023 Dec
TI  - Nuclear sperm integrity and ICSI prognosis in Tunisian patients with MMAF 
      syndrome (multiple morphological abnormalities of the sperm flagella).
PG  - 1429-1438
LID - 10.1080/14647273.2023.2251679 [doi]
AB  - Multiple Morphological Abnormalities of the Sperm Flagella (MMAF) is a severe 
      form of teratozoospermia associated with several sperm flagellar abnormalities. 
      The study included 52 patients with MMAF syndrome and a control group of 25 
      fertile men. The impact of nuclear sperm quality on intracytoplasmic sperm 
      injection (ICSI) results was studied in 20 couples. TUNEL assay was used to 
      assess sperm DNA fragmentation and aniline-blue staining was used to assess 
      chromatin condensation. To investigate chromosomal meiotic segregation, we used 
      fluorescence in situ hybridization (FISH). Semen morphology analysis revealed a 
      mosaic of multiple flagella morphological abnormalities, including 46.73% short 
      flagella, 16.22% bent flagella, 22.07% coiled flagella, and 10.90% absent 
      flagella, all of which were associated with a high percentage of sperm head 
      abnormalities. The mean DNA fragmentation index was substantially higher in 
      patients compared to controls (p = 0.001), whereas the rate of aniline 
      blue-reacted spermatozoa was not significantly different. There was a significant 
      difference in aneuploidy frequencies between the two groups (p < 0.05). Infertile 
      males with MMAF syndrome had lower sperm nuclear quality, which affected ICSI 
      results. As a result, better sperm selection procedures are being employed to 
      increase the success rate of assisted reproductive technologies (ART).
FAU - Braham, Asma
AU  - Braham A
AD  - Department of Cytogenetic and Reproductive Biology, Farhat Hached University 
      Hospital, Sousse, Tunisia.
FAU - Ghedir, Houda
AU  - Ghedir H
AD  - Department of Cytogenetic and Reproductive Biology, Farhat Hached University 
      Hospital, Sousse, Tunisia.
FAU - Ben Khedher, Myriam Beya
AU  - Ben Khedher MB
AD  - Department of Cytogenetic and Reproductive Biology, Farhat Hached University 
      Hospital, Sousse, Tunisia.
FAU - Ajina, Mounir
AU  - Ajina M
AD  - Reproductive Medicine Unit, University Hospital Farhat Hached, Sousse, Tunisia.
AD  - University of Medicine of Sousse, Farhat Hached Hospital, University of Sousse, 
      Sousse, Tunisia.
FAU - Saad, Ali
AU  - Saad A
AD  - Department of Cytogenetic and Reproductive Biology, Farhat Hached University 
      Hospital, Sousse, Tunisia.
AD  - University of Medicine of Sousse, Farhat Hached Hospital, University of Sousse, 
      Sousse, Tunisia.
FAU - Ibala-Romdhane, Samira
AU  - Ibala-Romdhane S
AD  - Department of Cytogenetic and Reproductive Biology, Farhat Hached University 
      Hospital, Sousse, Tunisia.
AD  - University of Medicine of Sousse, Farhat Hached Hospital, University of Sousse, 
      Sousse, Tunisia.
LA  - eng
PT  - Journal Article
DEP - 20230906
PL  - England
TA  - Hum Fertil (Camb)
JT  - Human fertility (Cambridge, England)
JID - 100888143
SB  - IM
MH  - Humans
MH  - Male
MH  - *Sperm Injections, Intracytoplasmic/methods
MH  - Semen
MH  - In Situ Hybridization, Fluorescence
MH  - Spermatozoa
MH  - *Infertility, Male/genetics
MH  - Prognosis
MH  - Flagella
OTO - NOTNLM
OT  - ART
OT  - DNA fragmentation
OT  - MMAF syndrome
OT  - chromatin condensation
OT  - sperm aneuploidy
EDAT- 2023/09/06 12:42
MHDA- 2024/01/25 06:43
CRDT- 2023/09/06 08:53
PHST- 2024/01/25 06:43 [medline]
PHST- 2023/09/06 12:42 [pubmed]
PHST- 2023/09/06 08:53 [entrez]
AID - 10.1080/14647273.2023.2251679 [doi]
PST - ppublish
SO  - Hum Fertil (Camb). 2023 Dec;26(6):1429-1438. doi: 10.1080/14647273.2023.2251679. 
      Epub 2023 Sep 6.