PMID- 37673757
OWN - NLM
STAT- MEDLINE
DCOM- 20231206
LR  - 20231217
IS  - 1473-0502 (Print)
IS  - 1473-0502 (Linking)
VI  - 62
IP  - 6
DP  - 2023 Dec
TI  - Association of HLA-DRB1 alleles with anti-D alloimmunization in RhD negative 
      pregnant women in India.
PG  - 103789
LID - S1473-0502(23)00193-3 [pii]
LID - 10.1016/j.transci.2023.103789 [doi]
AB  - BACKGROUND: Human leukocyte antigen (HLA) restriction plays an important role in 
      the susceptibility to alloimmunization against red blood cell (RBC) antigens. The 
      prevalence of anti-D alloimmunization in RhD negative pregnancy is still quite 
      high in our population. Thus, we planned this study to determine the association 
      of HLA-DRB1 alleles with anti-D alloimmunization in RhD negative pregnant women. 
      MATERIAL AND METHODS: RBC antibody screen (ABS) was performed for RhD negative 
      pregnant women attending the antenatal clinic our institute. Those with a 
      negative result were included in the 'non-alloimmunized' (NAL) group ('Control' 
      group), while those with anti-D alloantibody on performing antibody 
      identification were included in the alloimmunized (AL) group of the study (n = 50 
      each). ABS and identification were done using column agglutination technique. The 
      HLA-DRB1 typing was done by Luminex based reverse sequence specific 
      oligonucleotide probing (SSOP) using commercial kits. The HLA-DRB1 allele 
      frequency was compared in both the groups. RESULTS: There was a significant 
      difference between the two groups in terms of gravida (p < 0.001) and history of 
      anti-D immunoprophylaxis (p < 0.001). The frequency of HLA-DRB1*03 and 
      HLA-DRB1*04 alleles was significantly higher in the AL group than the NAL group: 
      40 % versus 18 % [Odds Ratio (OR): 3.04, 95 % CI: 1.21-7.6; p = 0.015] for 
      HLA-DRB1*03 alleles and 18 % versus 4 % (OR: 5.27, 95 % CI: 1.08-25.78, 
      p = 0.025) for HLA-DRB1*04 alleles. CONCLUSION: The frequency of HLADRB1*03 and 
      HLADRB1*04 alleles was significantly higher in RhD negative pregnant women 
      alloimmunized with anti-D alloantibody.
CI  - Copyright (c) 2023 Elsevier Ltd. All rights reserved.
FAU - Jain, Ashish
AU  - Jain A
AD  - Department of Transfusion Medicine, Postgraduate Institute of Medical Education 
      and Research (PGIMER), Chandigarh 160012, India. Electronic address: 
      ashishjain16@gmail.com.
FAU - Sharma, Ratti Ram
AU  - Sharma RR
AD  - Department of Transfusion Medicine, Postgraduate Institute of Medical Education 
      and Research (PGIMER), Chandigarh 160012, India.
FAU - Minz, Ranjana W
AU  - Minz RW
AD  - Department of Immunopathology, Postgraduate Institute of Medical Education and 
      Research (PGIMER), Chandigarh 160012, India.
FAU - Saha, Subhas Chandra
AU  - Saha SC
AD  - Department of Obstetrics and Gynecology, Postgraduate Institute of Medical 
      Education and Research (PGIMER), Chandigarh 160012, India.
LA  - eng
PT  - Journal Article
DEP - 20230822
PL  - England
TA  - Transfus Apher Sci
JT  - Transfusion and apheresis science : official journal of the World Apheresis 
      Association : official journal of the European Society for Haemapheresis
JID - 101095653
RN  - 0 (HLA-DRB1 Chains)
RN  - 0 (RHO(D) antibody)
RN  - 0 (Rho(D) Immune Globulin)
RN  - 0 (Isoantibodies)
MH  - Humans
MH  - Female
MH  - Pregnancy
MH  - HLA-DRB1 Chains/genetics
MH  - *Pregnant Women
MH  - Alleles
MH  - *Rho(D) Immune Globulin
MH  - Isoantibodies
MH  - Gene Frequency
OTO - NOTNLM
OT  - Anti-D alloimmunization
OT  - HLA-DRB1 alleles
OT  - Red cell alloimmunization
OT  - RhD negative pregnant women
COIS- Conflict of interest None.
EDAT- 2023/09/07 00:41
MHDA- 2023/12/06 06:42
CRDT- 2023/09/06 21:57
PHST- 2023/04/24 00:00 [received]
PHST- 2023/08/03 00:00 [revised]
PHST- 2023/08/18 00:00 [accepted]
PHST- 2023/12/06 06:42 [medline]
PHST- 2023/09/07 00:41 [pubmed]
PHST- 2023/09/06 21:57 [entrez]
AID - S1473-0502(23)00193-3 [pii]
AID - 10.1016/j.transci.2023.103789 [doi]
PST - ppublish
SO  - Transfus Apher Sci. 2023 Dec;62(6):103789. doi: 10.1016/j.transci.2023.103789. 
      Epub 2023 Aug 22.