PMID- 37674329
OWN - NLM
STAT- MEDLINE
DCOM- 20231109
LR  - 20231111
IS  - 1437-4315 (Electronic)
IS  - 1431-6730 (Linking)
VI  - 404
IP  - 11-12
DP  - 2023 Oct 26
TI  - Cytosolic RGG RNA-binding proteins are temperature sensitive flowering time 
      regulators in Arabidopsis.
PG  - 1069-1084
LID - 10.1515/hsz-2023-0171 [doi]
AB  - mRNA translation is tightly regulated by various classes of RNA-binding proteins 
      (RBPs) during development and in response to changing environmental conditions. 
      In this study, we characterize the arginine-glycine-glycine (RGG) motif 
      containing RBP family of Arabidopsis thaliana representing homologues of the 
      multifunctional translation regulators and ribosomal preservation factors Stm1 
      from yeast (ScStm1) and human SERBP1 (HsSERBP1). The Arabidopsis genome encodes 
      three RGG proteins named AtRGGA, AtRGGB and AtRGGC. While AtRGGA is ubiquitously 
      expressed, AtRGGB and AtRGGC are enriched in dividing cells. All AtRGGs localize 
      almost exclusively to the cytoplasm and bind with high affinity to ssRNA, while 
      being capable to interact with most nucleic acids, except dsRNA. A 
      protein-interactome study shows that AtRGGs interact with ribosomal proteins and 
      proteins involved in RNA processing and transport. In contrast to ScStm1, AtRGGs 
      are enriched in ribosome-free fractions in polysome profiles, suggesting 
      additional plant-specific functions. Mutant studies show that AtRGG proteins 
      differentially regulate flowering time, with a distinct and complex temperature 
      dependency for each AtRGG protein. In conclusion, we suggest that AtRGGs function 
      in fine-tuning translation efficiency to control flowering time and potentially 
      other developmental processes in response to environmental changes.
CI  - (c) 2023 the author(s), published by De Gruyter, Berlin/Boston.
FAU - Bleckmann, Andrea
AU  - Bleckmann A
AUID- ORCID: 0000-0002-0863-9840
AD  - Cell Biology and Plant Biochemistry, University of Regensburg, D-93053 
      Regensburg, Germany.
FAU - Spitzlberger, Nicole
AU  - Spitzlberger N
AD  - Cell Biology and Plant Biochemistry, University of Regensburg, D-93053 
      Regensburg, Germany.
FAU - Denninger, Philipp
AU  - Denninger P
AUID- ORCID: 0000-0003-3794-2360
AD  - Cell Biology and Plant Biochemistry, University of Regensburg, D-93053 
      Regensburg, Germany.
FAU - Ehrnsberger, Hans F
AU  - Ehrnsberger HF
AUID- ORCID: 0000-0002-0007-0485
AD  - Cell Biology and Plant Biochemistry, University of Regensburg, D-93053 
      Regensburg, Germany.
FAU - Wang, Lele
AU  - Wang L
AUID- ORCID: 0000-0002-8217-4332
AD  - Cell Biology and Plant Biochemistry, University of Regensburg, D-93053 
      Regensburg, Germany.
FAU - Bruckmann, Astrid
AU  - Bruckmann A
AUID- ORCID: 0000-0001-6082-2944
AD  - Biochemistry I, University of Regensburg, D-93053 Regensburg, Germany.
FAU - Reich, Stefan
AU  - Reich S
AD  - Biochemistry I, University of Regensburg, D-93053 Regensburg, Germany.
FAU - Holzinger, Philipp
AU  - Holzinger P
AD  - Cell Biology and Plant Biochemistry, University of Regensburg, D-93053 
      Regensburg, Germany.
FAU - Medenbach, Jan
AU  - Medenbach J
AUID- ORCID: 0000-0003-0749-7788
AD  - Biochemistry I, University of Regensburg, D-93053 Regensburg, Germany.
FAU - Grasser, Klaus D
AU  - Grasser KD
AUID- ORCID: 0000-0002-7080-5520
AD  - Cell Biology and Plant Biochemistry, University of Regensburg, D-93053 
      Regensburg, Germany.
FAU - Dresselhaus, Thomas
AU  - Dresselhaus T
AUID- ORCID: 0000-0001-6442-4302
AD  - Cell Biology and Plant Biochemistry, University of Regensburg, D-93053 
      Regensburg, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230908
PL  - Germany
TA  - Biol Chem
JT  - Biological chemistry
JID - 9700112
RN  - 0 (RNA-Binding Proteins)
RN  - TE7660XO1C (Glycine)
RN  - 0 (Arabidopsis Proteins)
SB  - IM
MH  - Humans
MH  - *Arabidopsis/genetics/metabolism
MH  - Temperature
MH  - RNA-Binding Proteins/chemistry
MH  - Cytosol/metabolism
MH  - Glycine/metabolism
MH  - *Arabidopsis Proteins/genetics/chemistry/metabolism
OTO - NOTNLM
OT  - RBP
OT  - RGG motif
OT  - SERBP1
OT  - Stm1
OT  - mRNA binding
OT  - ribosome
EDAT- 2023/09/07 06:42
MHDA- 2023/11/09 06:42
CRDT- 2023/09/07 02:01
PHST- 2023/04/03 00:00 [received]
PHST- 2023/08/03 00:00 [accepted]
PHST- 2023/11/09 06:42 [medline]
PHST- 2023/09/07 06:42 [pubmed]
PHST- 2023/09/07 02:01 [entrez]
AID - hsz-2023-0171 [pii]
AID - 10.1515/hsz-2023-0171 [doi]
PST - epublish
SO  - Biol Chem. 2023 Sep 8;404(11-12):1069-1084. doi: 10.1515/hsz-2023-0171. Print 
      2023 Oct 26.