PMID- 37679308
OWN - NLM
STAT- MEDLINE
DCOM- 20230928
LR  - 20230928
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 71
IP  - 38
DP  - 2023 Sep 27
TI  - One-Step Purification of IgE Epitope-Specific Antibody Using Immunomagnetic Beads 
      and Highly Sensitive Detection of Bovine beta-Lactoglobulin for the Prediction of 
      Milk Allergenicity in Foods.
PG  - 14068-14078
LID - 10.1021/acs.jafc.3c03461 [doi]
AB  - Bovine beta-lactoglobulin (BLG) is a common allergen found in milk, and the 
      immunoglobulin E (IgE) epitope plays a crucial role in cow milk allergy. 
      Therefore, targeting the IgE epitope could be useful in accurately detecting BLG 
      and assessing its allergenicity. However, producing an IgE epitope-specific 
      antibody (IgE-EsAb) through traditional methods requires complex and 
      time-consuming procedures. Here, IgE-EsAb was purified from rabbit anti-BLG sera 
      by immunomagnetic beads in one step. Then, a sandwich ELISA (sELISA) based on the 
      IgE-EsAb was developed to detect BLG and predict the potential milk allergenicity 
      in foods. The obtained IgE-EsAb could specifically recognize the target IgE 
      epitope of BLG and exhibited high affinity and specificity. The developed 
      IgE-EsAb-based sELISA demonstrated an ultra-wide linear range of 3.9-1.28 x 10(5) 
      ng/mL, with a limit of detection of 0.49 ng/mL for BLG. Additionally, the 
      proposed immunoassay showed high specificity and recoveries (91.24-109.61%). The 
      ability of the IgE-EsAb-based sELISA to evaluate the potential milk allergenicity 
      in foods was validated using sera from cow milk allergy patients. These results 
      suggest that immunomagnetic beads are an effective tool for rapidly obtaining the 
      IgE-EsAb, and our proposed sELISA could be a reliable and user-friendly method 
      for monitoring trace amounts of BLG and predicting the potential milk 
      allergenicity of food samples.
FAU - He, Shengfa
AU  - He S
AUID- ORCID: 0000-0002-7048-6136
AD  - School of Public Health and Health Management, Gannan Medical University, Ganzhou 
      341000, China.
FAU - Xiong, Meng
AU  - Xiong M
AD  - School of Public Health and Health Management, Gannan Medical University, Ganzhou 
      341000, China.
FAU - Li, Liming
AU  - Li L
AD  - First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China.
FAU - Yan, Yan
AU  - Yan Y
AD  - School of Public Health and Health Management, Gannan Medical University, Ganzhou 
      341000, China.
FAU - Li, Jinyu
AU  - Li J
AD  - First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China.
FAU - Feng, Ziling
AU  - Feng Z
AD  - School of Public Health and Health Management, Gannan Medical University, Ganzhou 
      341000, China.
FAU - Li, Yang
AU  - Li Y
AD  - School of Public Health and Health Management, Gannan Medical University, Ganzhou 
      341000, China.
FAU - Zhao, Jiangqiang
AU  - Zhao J
AD  - School of Public Health and Health Management, Gannan Medical University, Ganzhou 
      341000, China.
FAU - Dong, Yaping
AU  - Dong Y
AD  - School of Public Health and Health Management, Gannan Medical University, Ganzhou 
      341000, China.
FAU - Li, Xin
AU  - Li X
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang 330047, China.
FAU - Chen, Hongbing
AU  - Chen H
AUID- ORCID: 0000-0002-4845-383X
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang 330047, China.
FAU - Long, Caiyun
AU  - Long C
AD  - Ganzhou Center for Disease Control and Prevention, Ganzhou 341000, China.
LA  - eng
PT  - Journal Article
DEP - 20230907
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Allergens)
RN  - 0 (Epitopes)
RN  - 0 (Lactoglobulins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Female
MH  - Humans
MH  - Cattle
MH  - Animals
MH  - Rabbits
MH  - *Allergens
MH  - Epitopes
MH  - Lactoglobulins/analysis
MH  - *Milk Hypersensitivity
MH  - Immunoglobulin E
OTO - NOTNLM
OT  - IgE epitope-specific antibody
OT  - allergenicity assessment
OT  - bovine beta-lactoglobulin
OT  - immunomagnetic beads
OT  - sandwich ELISA
EDAT- 2023/09/08 00:41
MHDA- 2023/09/28 06:42
CRDT- 2023/09/07 22:53
PHST- 2023/09/28 06:42 [medline]
PHST- 2023/09/08 00:41 [pubmed]
PHST- 2023/09/07 22:53 [entrez]
AID - 10.1021/acs.jafc.3c03461 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2023 Sep 27;71(38):14068-14078. doi: 10.1021/acs.jafc.3c03461. 
      Epub 2023 Sep 7.