PMID- 37679987
OWN - NLM
STAT- MEDLINE
DCOM- 20230911
LR  - 20231016
IS  - 2589-451X (Electronic)
IS  - 0255-2922 (Print)
IS  - 0255-2922 (Linking)
VI  - 43
IP  - 5
DP  - 2023 Oct
TI  - Effect of Tuina along "bladder meridian" alleviating intervertebral disc 
      degeneration by regulating the transforming growth factor-beta1/Smad signaling 
      pathway in a rabbit model.
PG  - 991-1000
LID - 10.19852/j.cnki.jtcm.2023.05.005 [doi]
AB  - OBJECTIVE: The aim of this study was to investigate the protective effects of 
      Tuina (a traditional Chinese massage therapy) on intervertebral disc (IVD) 
      degeneration and the regulatory mechanisms of the transforming growth factor-beta1 
      (TGF-beta1)/small mothers against decapentaplegic (Smad) signaling pathway. METHODS: 
      Thirty New Zealand white rabbits were randomized into five groups: the control 
      group, model group, model + Tuina group (Tuina group), model + TGF-beta1 group 
      (TGF-beta1 group), and model + TGF-beta1 inhibitor SB431542 group (SB431542 group). The 
      model was established by posterolateral annulus fibrosus puncturing (AFP). 
      Recombinant TGF-beta1 and inhibitor SB431542 was injected into the TGF-beta1 group and 
      SB431542 group with a microsyringe, respectively. The rabbits in the Tuina group 
      received Tuina treatment along the bladder meridian for 4 weeks. Magnetic 
      resonance imaging (MRI) was performed on rabbits before AFP and after 4 weeks of 
      intervention. Lumbar IVDs (L2-L3 to L4-L5) were harvested after intervention. 
      Histopathological changes in the IVDs were measured by hematoxylin and eosin (HE) 
      staining. Type I collagen was analyzed by immunohistochemistry detection. The 
      expression level of matrix metalloproteinase-3 (MMP3) was determined by 
      enzyme-linked immunosorbent assay. Cell apoptosis was evaluated by terminal 
      deoxynucleotidyl transferase-mediated nick end labeling and Western blotting. 
      Real-time polymerase chain reaction and Western blotting were used to analyze the 
      expression of TGF-beta1 and Smad2/3/4 and a disintegrin and metalloproteinase with 
      thrombospondin motifs 5. RESULTS: Posterolateral AFP induced IVD degeneration in 
      rabbits with histopathological damage and noticeable changes in MRI images. Tuina 
      alleviated histo-pathological changes and reversed the expression of 
      extracellular matrix degeneration-related molecules and apoptosis-related 
      proteins. Furthermore, AFP induced the activation of TGF-beta1 and Smad2/3/4, 
      whereas Tuina therapy markedly reduced the protein expression of Smad2/3 and the 
      gene expression of TGF-beta1 and Smad2/3/4. Additionally, the TGF-beta1/Smad signaling 
      pathway was activated in the TGF-beta1 group, while the TGF-beta1/Smad signaling 
      pathway was inhibited in the SB431542 group. CONCLUSION: Posterolateral AFP 
      induced disc degeneration as determined by MRI assessment and histological 
      analysis. Tuina alleviated disc degeneration, possibly by inhibiting the fibrotic 
      response mediated by the TGF-beta1/Smad pathway, thus alleviating extracellular 
      matrix degeneration and reducing cell apoptosis.
FAU - Chengguo, S U
AU  - Chengguo SU
AD  - Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, 
      Chengdu 610075, China.
FAU - Xiaoyan, Zhao
AU  - Xiaoyan Z
AD  - Clinical Medical School, Affiliated Hospital of Chengdu University of Traditional 
      Chinese Medicine, Chengdu 610075, China.
FAU - Jiangnan, Y E
AU  - Jiangnan YE
AD  - Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, 
      Chengdu 610075, China.
FAU - Xin, Zhang
AU  - Xin Z
AD  - Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, 
      Chengdu 610075, China.
FAU - Yuqing, Jiang
AU  - Yuqing J
AD  - Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, 
      Chengdu 610075, China.
FAU - Junjie, Guo
AU  - Junjie G
AD  - Clinical Medical School, Affiliated Hospital of Chengdu University of Traditional 
      Chinese Medicine, Chengdu 610075, China.
FAU - Xiyuan, Zhang
AU  - Xiyuan Z
AD  - Clinical Medical School, Affiliated Hospital of Chengdu University of Traditional 
      Chinese Medicine, Chengdu 610075, China.
FAU - Wenchuan, Q I
AU  - Wenchuan QI
AD  - Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, 
      Chengdu 610075, China.
FAU - Jun, Zhu
AU  - Jun Z
AD  - Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, 
      Chengdu 610075, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - J Tradit Chin Med
JT  - Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan
JID - 8211546
RN  - 0 (4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (alpha-Fetoproteins)
SB  - IM
MH  - Rabbits
MH  - Animals
MH  - Female
MH  - Humans
MH  - Transforming Growth Factor beta1/genetics
MH  - *Intervertebral Disc Degeneration/genetics/therapy
MH  - *Meridians
MH  - Mothers
MH  - Urinary Bladder
MH  - alpha-Fetoproteins
MH  - Signal Transduction
PMC - PMC10465829
OTO - NOTNLM
OT  - Smad proteins
OT  - Tuina
OT  - apoptosis
OT  - extracellular matrix degradation
OT  - intervertebral disc degeneration
OT  - transforming growth factor beta1
EDAT- 2023/09/08 06:42
MHDA- 2023/09/11 06:41
PMCR- 2023/10/15
CRDT- 2023/09/08 02:08
PHST- 2023/09/11 06:41 [medline]
PHST- 2023/09/08 06:42 [pubmed]
PHST- 2023/09/08 02:08 [entrez]
PHST- 2023/10/15 00:00 [pmc-release]
AID - 1693294259256-892950836 [pii]
AID - 10.19852/j.cnki.jtcm.2023.05.005 [doi]
PST - ppublish
SO  - J Tradit Chin Med. 2023 Oct;43(5):991-1000. doi: 
      10.19852/j.cnki.jtcm.2023.05.005.