PMID- 37682717
OWN - NLM
STAT- MEDLINE
DCOM- 20240308
LR  - 20240308
IS  - 2666-1667 (Electronic)
IS  - 2666-1667 (Linking)
VI  - 4
IP  - 3
DP  - 2023 Sep 15
TI  - Protocol for in vivo and in vitro activation of NLRP3 inflammasome in mice using 
      monosodium urate.
PG  - 102554
LID - S2666-1667(23)00521-X [pii]
LID - 10.1016/j.xpro.2023.102554 [doi]
LID - 102554
AB  - Although intraperitoneal injection of monosodium urate (MSU) is an effective 
      model for studying peritonitis, its establishment remains challenging. Here, we 
      present a protocol for using MSU to activate the NLRP3 inflammasome in 
      bone-marrow-derived macrophages (BMDMs) and to induce peritonitis in mice. We 
      describe steps for isolating and culturing BMDMs, preparing MSU crystals, and 
      activating the NLRP3 inflammasome using western blot and ELISA. We then detail 
      procedures for inducing peritonitis and testing for relevant indicators using 
      flow cytometry and ELISA. For complete details on the use and execution of this 
      protocol, please refer to Huang et al. (2023).(1).
CI  - Copyright (c) 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
FAU - Liu, Didi
AU  - Liu D
AD  - Wuxi School of Medicine, Jiangnan University, Jiangsu 214000, China.
FAU - Huang, Yi
AU  - Huang Y
AD  - Wuxi School of Medicine, Jiangnan University, Jiangsu 214000, China. Electronic 
      address: hy527@jiangnan.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20230907
PL  - United States
TA  - STAR Protoc
JT  - STAR protocols
JID - 101769501
RN  - 0 (Inflammasomes)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 268B43MJ25 (Uric Acid)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Inflammasomes
MH  - NLR Family, Pyrin Domain-Containing 3 Protein
MH  - Uric Acid/pharmacology
MH  - Macrophages
MH  - *Peritonitis/chemically induced
PMC - PMC10493585
OTO - NOTNLM
OT  - Cell Biology
OT  - Immunology
OT  - Metabolism
COIS- Declaration of interests The authors declare no competing interests.
EDAT- 2023/09/08 18:42
MHDA- 2024/03/08 06:43
PMCR- 2023/09/07
CRDT- 2023/09/08 12:36
PHST- 2023/04/26 00:00 [received]
PHST- 2023/06/27 00:00 [revised]
PHST- 2023/08/11 00:00 [accepted]
PHST- 2024/03/08 06:43 [medline]
PHST- 2023/09/08 18:42 [pubmed]
PHST- 2023/09/08 12:36 [entrez]
PHST- 2023/09/07 00:00 [pmc-release]
AID - S2666-1667(23)00521-X [pii]
AID - 102554 [pii]
AID - 10.1016/j.xpro.2023.102554 [doi]
PST - ppublish
SO  - STAR Protoc. 2023 Sep 15;4(3):102554. doi: 10.1016/j.xpro.2023.102554. Epub 2023 
      Sep 7.