PMID- 37685847
OWN - NLM
STAT- MEDLINE
DCOM- 20230911
LR  - 20230911
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 17
DP  - 2023 Aug 22
TI  - The Combination of Gold and Silver Food Nanoparticles with Gluten Peptides Alters 
      the Autophagic Pathway in Intestinal Crypt-like Cells.
LID - 10.3390/ijms241713040 [doi]
LID - 13040
AB  - Metallic nanoparticles (mNPs) are widely used as food additives and can interact 
      with gliadin triggering an immune response, but evaluation of the effects on 
      crypts, hypertrophic in celiac subjects, is still lacking. This study evaluated 
      the effects of gold and silver mNPs in combination with gliadin on crypt-like 
      cells (HIEC-6). Transmission electron microscopy (TEM) was used to evaluate 
      gliadin-mNP aggregates in cells. Western blot and immunofluorescence analysis 
      assessed autophagy-related molecule levels (p62, LC3, beclin-1, EGFR). Lysosome 
      functionality was tested with acridine orange (AO) and Magic Red assays. TEM 
      identified an increase in autophagic vacuoles after exposure to gliadin + mNPs, 
      as also detected by significant increments in LC3-II and p62 expression. 
      Immunofluorescence confirmed the presence of mature autophagosomes, showing LC3 
      and p62 colocalization, indicating an altered autophagic flux, further assessed 
      with EGFR degradation, AO and Magic Red assays. The results showed a significant 
      reduction in lysosomal enzyme activity and a modest reduction in acidity. Thus, 
      gliadin + mNPs can block the autophagic flux inducing a lysosomal defect. The 
      alteration of this pathway, essential for cell function, can lead to cell damage 
      and death. The potential effects of this copresence in food should be further 
      characterized to avoid a negative impact on celiac disease subjects.
FAU - Mancuso, Clara
AU  - Mancuso C
AUID- ORCID: 0000-0001-7464-1749
AD  - School of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy.
AD  - Laboratory of Intestinal Physiopathology, Faculty of Medicine and Health 
      Sciences, Universite de Sherbrooke, Sherbrooke, QC J1H 5H4, Canada.
FAU - Tremblay, Eric
AU  - Tremblay E
AD  - Laboratory of Intestinal Physiopathology, Faculty of Medicine and Health 
      Sciences, Universite de Sherbrooke, Sherbrooke, QC J1H 5H4, Canada.
FAU - Gnodi, Elisa
AU  - Gnodi E
AUID- ORCID: 0000-0002-4390-6633
AD  - School of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy.
FAU - Jean, Steve
AU  - Jean S
AUID- ORCID: 0000-0001-6881-5781
AD  - Department of Immunology and Cell Biology, Faculty of Medicine and Health 
      Sciences, Universite de Sherbrooke, Sherbrooke, QC J1H 5H4, Canada.
FAU - Beaulieu, Jean-Francois
AU  - Beaulieu JF
AUID- ORCID: 0000-0002-8977-3629
AD  - Laboratory of Intestinal Physiopathology, Faculty of Medicine and Health 
      Sciences, Universite de Sherbrooke, Sherbrooke, QC J1H 5H4, Canada.
FAU - Barisani, Donatella
AU  - Barisani D
AUID- ORCID: 0000-0003-4592-6221
AD  - School of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy.
LA  - eng
GR  - MOP-136991/CAPMC/CIHR/Canada
GR  - PJT-162423/CAPMC/CIHR/Canada
PT  - Journal Article
DEP - 20230822
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 7440-57-5 (Gold)
RN  - 8002-80-0 (Glutens)
RN  - 3M4G523W1G (Silver)
RN  - 9007-90-3 (Gliadin)
RN  - F30N4O6XVV (Acridine Orange)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Humans
MH  - *Gold
MH  - Glutens
MH  - Silver
MH  - Gliadin
MH  - Autophagy
MH  - Acridine Orange
MH  - *Nanoparticles
MH  - ErbB Receptors
PMC - PMC10487529
OTO - NOTNLM
OT  - autophagy
OT  - celiac disease
OT  - dietary metallic nanoparticles
OT  - food additives
OT  - gluten peptides
OT  - lysosome dysfunction
COIS- The authors declare no conflict of interest.
EDAT- 2023/09/09 11:43
MHDA- 2023/09/11 06:42
PMCR- 2023/08/22
CRDT- 2023/09/09 01:10
PHST- 2023/07/31 00:00 [received]
PHST- 2023/08/11 00:00 [revised]
PHST- 2023/08/18 00:00 [accepted]
PHST- 2023/09/11 06:42 [medline]
PHST- 2023/09/09 11:43 [pubmed]
PHST- 2023/09/09 01:10 [entrez]
PHST- 2023/08/22 00:00 [pmc-release]
AID - ijms241713040 [pii]
AID - ijms-24-13040 [pii]
AID - 10.3390/ijms241713040 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Aug 22;24(17):13040. doi: 10.3390/ijms241713040.