PMID- 37686399
OWN - NLM
STAT- MEDLINE
DCOM- 20230911
LR  - 20230912
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 17
DP  - 2023 Sep 2
TI  - Cellular Immunotherapy in Mice Prevents Maternal Hypertension and Restores 
      Anti-Inflammatory Cytokine Balance in Maternal and Fetal Tissues.
LID - 10.3390/ijms241713594 [doi]
LID - 13594
AB  - Preeclampsia is the leading cause of maternal-fetal morbidity worldwide. The 
      concept that persistent feto-placental intolerance is important in the 
      pathogenesis of preeclampsia (PreE) has been demonstrated by our lab and others. 
      Arginine vasopressin (AVP) infusion during pregnancy induces cardiovascular, 
      renal, and T helper (T(H)) cell alterations in mice consistent with human PreE. 
      In addition to their conventional immuno-stimulatory role, dendritic cells (DCs) 
      also play a vital role in immune tolerance. In contrast to conventional DCs, 
      regulatory DCs (DCregs) express low levels of co-stimulatory markers, produce 
      anti-inflammatory cytokines, induce T regulatory (Treg) cells, and promote 
      tolerance. In mice, DCregs prevent pro-inflammatory responses and induce 
      antigen-specific tolerance. Given these known functions of DCregs, we hypothesize 
      that DCregs will prevent the development of AVP-induced PreE in mice. C57BL/6J 
      females were infused with AVP (24 ng/h) or saline throughout gestation via an 
      osmotic minipump. Bone-marrow-derived DCregs were injected into AVP-infused dams 
      at the time of the pump implantation or on gestational day (GD) 7. The blood 
      pressure of the mice was taken throughout their pregnancy. The maternal urine 
      proteins and T(H)-associated cytokines in maternal and fetal tissues were 
      measured on GD 18. The treatment with DCregs effectively prevented the elevation 
      of maternal blood pressure, proteinuria, and fetal growth restriction that were 
      observed in AVP-infused dams. Furthermore, we noted a reduction in the 
      pro-inflammatory T(H)-associated cytokines IFNgamma and IL-17, while 
      anti-inflammatory cytokines IL-4, IL-10, and TGFbeta showed an increase following 
      DCreg treatment. These outcomes provide strong evidence supporting the potential 
      of DCregs as a valuable therapeutic approach in addressing PreE.
FAU - Gray, Gabrielle
AU  - Gray G
AD  - Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA.
FAU - Scroggins, Douglas G
AU  - Scroggins DG
AD  - School of Medicine, Department of Biomedical Sciences, University of Minnesota 
      Duluth, Duluth, MN 55812, USA.
FAU - Wilson, Katlin T
AU  - Wilson KT
AD  - School of Medicine, Department of Biomedical Sciences, University of Minnesota 
      Duluth, Duluth, MN 55812, USA.
FAU - Scroggins, Sabrina M
AU  - Scroggins SM
AUID- ORCID: 0000-0001-5842-0575
AD  - School of Medicine, Department of Biomedical Sciences, University of Minnesota 
      Duluth, Duluth, MN 55812, USA.
LA  - eng
GR  - K01 HL155240/HL/NHLBI NIH HHS/United States
GR  - UL1 TR002494/TR/NCATS NIH HHS/United States
GR  - UL1TR002494/TR/NCATS NIH HHS/United States
GR  - KL2 TR002492/TR/NCATS NIH HHS/United States
GR  - KL2TR002492/TR/NCATS NIH HHS/United States
PT  - Journal Article
DEP - 20230902
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Cytokines)
RN  - 113-79-1 (Arginine Vasopressin)
RN  - 0 (Anti-Inflammatory Agents)
SB  - IM
MH  - Pregnancy
MH  - Humans
MH  - Female
MH  - Animals
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Cytokines
MH  - *Pre-Eclampsia/therapy
MH  - Placenta
MH  - Immunotherapy
MH  - Fetus
MH  - Arginine Vasopressin
MH  - Anti-Inflammatory Agents
MH  - *Hypertension
PMC - PMC10487605
OTO - NOTNLM
OT  - cellular therapy
OT  - cytokines
OT  - inflammation
OT  - preeclampsia
OT  - regulatory dendritic cells
COIS- The authors declare no conflict of interest.
EDAT- 2023/09/09 11:45
MHDA- 2023/09/11 06:42
PMCR- 2023/09/02
CRDT- 2023/09/09 01:13
PHST- 2023/08/15 00:00 [received]
PHST- 2023/08/29 00:00 [revised]
PHST- 2023/09/01 00:00 [accepted]
PHST- 2023/09/11 06:42 [medline]
PHST- 2023/09/09 11:45 [pubmed]
PHST- 2023/09/09 01:13 [entrez]
PHST- 2023/09/02 00:00 [pmc-release]
AID - ijms241713594 [pii]
AID - ijms-24-13594 [pii]
AID - 10.3390/ijms241713594 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Sep 2;24(17):13594. doi: 10.3390/ijms241713594.