PMID- 37691924
OWN - NLM
STAT- MEDLINE
DCOM- 20230912
LR  - 20230913
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 14
DP  - 2023
TI  - Integrative transcriptomic profiling of mRNA, miRNA, circRNA, and lncRNA in 
      alveolar macrophages isolated from PRRSV-infected porcine.
PG  - 1258778
LID - 10.3389/fimmu.2023.1258778 [doi]
LID - 1258778
AB  - INTRODUCTION: The porcine reproductive and respiratory syndrome virus (PRRSV) 
      continues to pose a significant threat to the global swine industry, attributed 
      largely to its immunosuppressive properties and the chronic nature of its 
      infection. The absence of effective vaccines and therapeutics amplifies the 
      urgency to deepen our comprehension of PRRSV's intricate pathogenic mechanisms. 
      Previous transcriptomic studies, although informative, are partially constrained 
      by their predominant reliance on in vitro models or lack of long-term infections. 
      Moreover, the role of circular RNAs (circRNAs) during PRRSV invasion is yet to be 
      elucidated. METHODS: In this study, we employed an in vivo approach, exposing 
      piglets to a PRRSV challenge over varied durations of 3, 7, or 21 days. 
      Subsequently, porcine alveolar macrophages were isolated for a comprehensive 
      transcriptomic investigation, examining the expression patterns of mRNAs, miRNAs, 
      circRNAs, and long non-coding RNAs (lncRNAs). RESULTS: Differentially expressed 
      RNAs from all four categories were identified, underscoring the dynamic interplay 
      among these RNA species during PRRSV infection. Functional enrichment analyses 
      indicate that these differentially expressed RNAs, as well as their target genes, 
      play a pivotal role in immune related pathways. For the first time, we integrated 
      circRNAs into the lncRNA-miRNA-mRNA relationship, constructing a competitive 
      endogenous RNA (ceRNA) network. Our findings highlight the immune-related genes, 
      CTLA4 and SAMHD1, as well as their associated miRNAs, lncRNAs, and circRNAs, 
      suggesting potential therapeutic targets for PRRS. Importantly, we corroborated 
      the expression patterns of selected RNAs through RT-qPCR, ensuring consistency 
      with our transcriptomic sequencing data. DISCUSSION: This study sheds lights on 
      the intricate RNA interplay during PRRSV infection and provides a solid 
      foundation for future therapeutic strategizing.
CI  - Copyright (c) 2023 Peng, Xia, Wei, Zeng, Zou, Hu, Xu, Huang, Geng, Hu, Cao and 
      Zhang.
FAU - Peng, Ouyang
AU  - Peng O
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Xia, Yu
AU  - Xia Y
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Wei, Ying
AU  - Wei Y
AD  - College of Animal Science and Technology/Luoyang Key Laboratory of Live Carrier 
      Biomaterial and Animal Disease Prevention and Control, Henan University of 
      Science and Technology, Luoyang, China.
FAU - Zeng, Siying
AU  - Zeng S
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Zou, Chuangchao
AU  - Zou C
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Hu, Fangyu
AU  - Hu F
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Xu, Qiuping
AU  - Xu Q
AD  - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene 
      Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, 
      China.
FAU - Huang, Yihui
AU  - Huang Y
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Geng, Rui
AU  - Geng R
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Hu, Guangli
AU  - Hu G
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Cao, Yongchang
AU  - Cao Y
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Zhang, Hao
AU  - Zhang H
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University, Guangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230824
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (RNA, Circular)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Animals
MH  - Swine
MH  - RNA, Circular/genetics
MH  - *MicroRNAs/genetics
MH  - RNA, Messenger/genetics
MH  - *RNA, Long Noncoding/genetics
MH  - *Porcine respiratory and reproductive syndrome virus/genetics
MH  - Transcriptome
MH  - Macrophages, Alveolar
PMC - PMC10491896
OTO - NOTNLM
OT  - ceRNA network
OT  - circular RNA
OT  - functional enrichment analysis
OT  - immune response
OT  - integrative transcriptomic profiling
OT  - porcine reproductive and respiratory syndrome virus
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/09/11 06:42
MHDA- 2023/09/12 06:42
PMCR- 2023/01/01
CRDT- 2023/09/11 04:29
PHST- 2023/07/14 00:00 [received]
PHST- 2023/08/09 00:00 [accepted]
PHST- 2023/09/12 06:42 [medline]
PHST- 2023/09/11 06:42 [pubmed]
PHST- 2023/09/11 04:29 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2023.1258778 [doi]
PST - epublish
SO  - Front Immunol. 2023 Aug 24;14:1258778. doi: 10.3389/fimmu.2023.1258778. 
      eCollection 2023.