PMID- 37694332
OWN - NLM
STAT- MEDLINE
DCOM- 20230912
LR  - 20230912
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 45
IP  - 1
DP  - 2023
TI  - Suppression of Kruppel-like factor 5 basal expression by CREB1 binding to far 
      distal element.
PG  - 81-94
LID - 10.3233/TUB-230017 [doi]
AB  - BACKGROUND: Kruppel-like factor 5 (KLF5) is a transcription factor regulating the 
      proliferation and differentiation of epithelial cells, and its uncontrolled 
      expression is closely associated with carcinoma progression. Sp3 binding to the 
      minimal essential region (MER) of KLF5 gene is critical for KLF5 basal 
      expression, but the expression control mechanism is unknown. OBJECTIVE: This 
      study aimed to identify a regulatory region for KLF5 basal expression and the 
      binding protein in carcinoma cells by analyzing the promoter upstream region. 
      METHODS: Reporter assays determined the silencer region. The protein binding to 
      the region was identified by database analysis and ChIP assay. The protein 
      mediating the interaction between the region and the MER was confirmed through 
      chromosome conformation capture (3 C) on ChIP assay. The effects of the protein 
      on KLF5 expression were analyzed using qRT-PCR and western blot. RESULTS: 
      Reporter assay localized the 425-region from upstream KLF5 gene as the silencer. 
      Database analysis and ChIP assay found CREB1 binding to the 425-region. CREB1 
      siRNA or mutation of CREB1-binding site in the 425-region increased luciferase 
      activities and decreased the binding to 425-region. 3 C on ChIP assay showed that 
      CREB1 mediated interaction of the 425-region and the MER. CREB1 overexpression 
      decreased endogenous KLF5 expression and luciferase activity. CONCLUSIONS: The 
      425-region is the silencer of KLF5 basal expression, and CREB1 binding suppresses 
      the expression.
FAU - Mihara, Nozomi
AU  - Mihara N
AD  - Department of Biochemistry, The Nippon Dental University School of Life Dentistry 
      at Tokyo, Tokyo, Japan.
FAU - Imai, Kazushi
AU  - Imai K
AD  - Department of Biochemistry, The Nippon Dental University School of Life Dentistry 
      at Tokyo, Tokyo, Japan.
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental 
      Biology and Medicine
JID - 8409922
RN  - 0 (CREB1 protein, human)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - EC 1.13.12.- (Luciferases)
RN  - 0 (KLF5 protein, human)
SB  - IM
MH  - Humans
MH  - *Carcinoma
MH  - Cell Differentiation
MH  - *Cyclic AMP Response Element-Binding Protein/genetics
MH  - *Kruppel-Like Transcription Factors/genetics
MH  - Luciferases
OTO - NOTNLM
OT  - CREB1
OT  - KLF5
OT  - oral carcinoma
OT  - suppression
OT  - transcriptional regulation
EDAT- 2023/09/11 06:42
MHDA- 2023/09/12 06:42
CRDT- 2023/09/11 05:08
PHST- 2023/09/12 06:42 [medline]
PHST- 2023/09/11 06:42 [pubmed]
PHST- 2023/09/11 05:08 [entrez]
AID - TUB230017 [pii]
AID - 10.3233/TUB-230017 [doi]
PST - ppublish
SO  - Tumour Biol. 2023;45(1):81-94. doi: 10.3233/TUB-230017.