PMID- 37695103 OWN - NLM STAT- MEDLINE DCOM- 20240509 LR - 20240510 IS - 1522-2586 (Electronic) IS - 1053-1807 (Print) IS - 1053-1807 (Linking) VI - 59 IP - 6 DP - 2024 Jun TI - Quantitative Assessment of Peripheral Oxidative Metabolism With a New Dynamic (1)H MRI Technique: A Pilot Study in People With and Without Diabetes Mellitus. PG - 2091-2100 LID - 10.1002/jmri.28996 [doi] AB - BACKGROUND: Type 2 diabetes mellitus (T2DM) is linked to impaired mitochondrial function. Chemical exchange saturation transfer (CEST) magnetic resonance imaging (MRI) is a gadolinium-contrast-free (1)H method to assess mitochondrial function by measuring low-concentration metabolites. A CEST MRI-based technique may serve as a non-invasive proxy for assessing mitochondrial health. HYPOTHESIS: A (1)H CEST MRI technique may detect significant differences in in vivo skeletal muscle phosphocreatine (SMPCr) kinetics between healthy volunteers and T2DM patients undergoing standardized isometric exercise. STUDY TYPE: Cross-sectional study. SUBJECTS: Seven subjects without T2DM (T2DM-) and seven age, sex, and BMI-matched subjects with T2DM (T2DM+). FIELD STRENGTH/SEQUENCE: Single-shot rapid acquisition with refocusing echoes (RARE) and single-shot gradient-echo sequences, 3 T. ASSESSMENT: Subjects underwent a rest-exercise-recovery imaging protocol to dynamically acquire SMPCr maps in calf musculature. Medial gastrocnemius (MG) and soleus SMPCr concentrations were plotted over time, and SMPCr recovery time, tau , was determined. Mitochondrial function index was calculated as the ratio of resting SMPCr to tau . Participants underwent a second exercise protocol for imaging of skeletal muscle blood flow (SMBF), and its association with SMPCr was assessed. STATISTICAL TESTS: Unpaired t-tests and Pearson correlation coefficient. A P value <0.05 was considered statistically significant. RESULTS: SMPCr concentrations in MG and soleus displayed expected declines during exercise and returns to baseline during recovery. tau was significantly longer in the T2DM+ cohort (MG 83.5 +/- 25.8 vs. 54.0 +/- 21.1, soleus 90.5 +/- 18.9 vs. 51.2 +/- 14.5). The mitochondrial function index in the soleus was significantly lower in the T2DM+ cohort (0.33 +/- 0.08 vs. 0.66 +/- 0.19). SMBF was moderately correlated with the SMPCr in T2DM-; this correlation was not significant in T2DM+ (r = -0.23, P = 0.269). CONCLUSION: The CEST MRI method is feasible for quantifying SMPCr in peripheral muscle tissue. T2DM+ individuals had significantly lower oxidative capacities than T2DM- individuals. In T2DM, skeletal muscle metabolism appeared to be decoupled from perfusion. LEVEL OF EVIDENCE: 1 TECHNICAL EFFICACY: Stage 1. CI - (c) 2023 International Society for Magnetic Resonance in Medicine. FAU - Wahidi, Ryan AU - Wahidi R AUID- ORCID: 0000-0002-3298-9146 AD - Department of Surgery, Section of Vascular Surgery, Washington University School of Medicine, Saint Louis, Missouri, USA. FAU - Zhang, Yi AU - Zhang Y AD - Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, China. FAU - Li, Ran AU - Li R AUID- ORCID: 0000-0001-8647-1580 AD - Mallinckrodt Institute of Radiology, Washington University School of Medicine, Saint Louis, Missouri, USA. FAU - Xu, Jiadi AU - Xu J AD - Department of Radiology, John Hopkins University, Baltimore, Maryland, USA. FAU - Zayed, Mohamed A AU - Zayed MA AD - Department of Surgery, Section of Vascular Surgery, Washington University School of Medicine, Saint Louis, Missouri, USA. FAU - Hastings, Mary K AU - Hastings MK AD - Physical Therapy Program, Washington University School of Medicine, Saint Louis, Missouri, USA. FAU - Zheng, Jie AU - Zheng J AD - Mallinckrodt Institute of Radiology, Washington University School of Medicine, Saint Louis, Missouri, USA. LA - eng GR - R01 DK105322/DK/NIDDK NIH HHS/United States GR - R01 HL153262/HL/NHLBI NIH HHS/United States GR - R03 EB028415/EB/NIBIB NIH HHS/United States GR - R21 AR065672/AR/NIAMS NIH HHS/United States GR - R21 AR065672/AR/NIAMS NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20230911 PL - United States TA - J Magn Reson Imaging JT - Journal of magnetic resonance imaging : JMRI JID - 9105850 RN - 020IUV4N33 (Phosphocreatine) SB - IM MH - Humans MH - Pilot Projects MH - Male MH - *Muscle, Skeletal/diagnostic imaging/metabolism MH - Female MH - *Magnetic Resonance Imaging/methods MH - Cross-Sectional Studies MH - Middle Aged MH - *Diabetes Mellitus, Type 2/diagnostic imaging/metabolism MH - *Phosphocreatine/metabolism MH - Adult MH - Exercise/physiology MH - Oxidation-Reduction MH - Aged PMC - PMC10925551 MID - NIHMS1931294 OTO - NOTNLM OT - blood flow OT - diabetes mellitus OT - phosphocreatine OT - skeletal muscle EDAT- 2023/09/11 12:42 MHDA- 2024/05/10 05:43 PMCR- 2025/03/11 CRDT- 2023/09/11 09:23 PHST- 2023/08/19 00:00 [revised] PHST- 2023/01/31 00:00 [received] PHST- 2023/08/23 00:00 [accepted] PHST- 2025/03/11 00:00 [pmc-release] PHST- 2024/05/10 05:43 [medline] PHST- 2023/09/11 12:42 [pubmed] PHST- 2023/09/11 09:23 [entrez] AID - 10.1002/jmri.28996 [doi] PST - ppublish SO - J Magn Reson Imaging. 2024 Jun;59(6):2091-2100. doi: 10.1002/jmri.28996. Epub 2023 Sep 11.