PMID- 37696370
OWN - NLM
STAT- MEDLINE
DCOM- 20231123
LR  - 20231123
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 253
IP  - Pt 2
DP  - 2023 Dec 31
TI  - PRRSV alters m(6)A methylation and alternative splicing to regulate immune, 
      extracellular matrix-associated function.
PG  - 126741
LID - S0141-8130(23)03638-3 [pii]
LID - 10.1016/j.ijbiomac.2023.126741 [doi]
AB  - The alternative splicing and N6-methyladenosine (m(6)A) modifications occurring 
      during porcine reproductive and respiratory syndrome virus (PRRSV) infections 
      remain poorly understood. Transcriptome and MeRIP-seq analyses were performed to 
      identify the gene expression changes, splicing and m6A modifications in the lungs 
      of PRRSV-infected pigs. In total, 1624 differentially expressed genes (DEGs) were 
      observed between PRRSV-infected and uninfected pigs. We observed significant 
      alterations in alternative splicing (54,367 events) and m6A modifications (2265 
      DASEs) in numerous genes, including LMO7, SLC25A27, ZNF185, and ECM1, during 
      PRRSV infection. LMO7 and ZNF185 exhibited alternative splicing variants and 
      reduced mRNA expression levels following PRRSV infection. Notably, LMO7 inhibited 
      c-JUN, SMAD3, and FAK expression, whereas ZNF185 affected the expression of FAK, 
      CDH1, and GSK3beta downstream. Additionally, ECM1 influenced FAK expression by 
      targeting ITGB3 and AKT2, suggesting its involvement in extracellular matrix 
      accumulation through the ITGB3-AKT2/FAK pathway. These changes may facilitate 
      viral invasion and replication by modulating the expression of genes and proteins 
      participating in crucial cellular processes associated with immunity and the 
      extracellular matrix. We highlight the importance of these genes and their 
      associated pathways in PRRSV infections and suggest that targeting these may be a 
      promising therapeutic approach for treating viral infections.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Lin, Chenghong
AU  - Lin C
AD  - Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise 
      Breeding, Key Laboratory of Animal Molecular Design and Precise Breeding of 
      Guangdong Higher Education Institutes, School of Life Science and Engineering, 
      Foshan University, Foshan 528225, China.
FAU - Zeng, Mu
AU  - Zeng M
AD  - Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise 
      Breeding, Key Laboratory of Animal Molecular Design and Precise Breeding of 
      Guangdong Higher Education Institutes, School of Life Science and Engineering, 
      Foshan University, Foshan 528225, China.
FAU - Song, Jia
AU  - Song J
AD  - Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise 
      Breeding, Key Laboratory of Animal Molecular Design and Precise Breeding of 
      Guangdong Higher Education Institutes, School of Life Science and Engineering, 
      Foshan University, Foshan 528225, China.
FAU - Li, Hua
AU  - Li H
AD  - Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise 
      Breeding, Key Laboratory of Animal Molecular Design and Precise Breeding of 
      Guangdong Higher Education Institutes, School of Life Science and Engineering, 
      Foshan University, Foshan 528225, China.
FAU - Feng, Zheng
AU  - Feng Z
AD  - Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise 
      Breeding, Key Laboratory of Animal Molecular Design and Precise Breeding of 
      Guangdong Higher Education Institutes, School of Life Science and Engineering, 
      Foshan University, Foshan 528225, China.
FAU - Li, Kui
AU  - Li K
AD  - Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key 
      Laboratory of Livestock and Poultry Multi-omics of MARA, Agricultural Genomics 
      Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 
      518124, China. Electronic address: likui@caas.cn.
FAU - Pei, Yangli
AU  - Pei Y
AD  - Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise 
      Breeding, Key Laboratory of Animal Molecular Design and Precise Breeding of 
      Guangdong Higher Education Institutes, School of Life Science and Engineering, 
      Foshan University, Foshan 528225, China. Electronic address: peiyl@fosu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20230909
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
SB  - IM
MH  - Swine
MH  - Animals
MH  - *Porcine respiratory and reproductive syndrome virus/genetics
MH  - *Porcine Reproductive and Respiratory Syndrome
MH  - Gene Expression Profiling
MH  - Methylation
MH  - Alternative Splicing/genetics
OTO - NOTNLM
OT  - Alternative splicing
OT  - N6-methyladenosine
OT  - PRRSV
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/09/12 00:42
MHDA- 2023/11/23 06:42
CRDT- 2023/09/11 19:18
PHST- 2022/11/17 00:00 [received]
PHST- 2023/08/07 00:00 [revised]
PHST- 2023/08/25 00:00 [accepted]
PHST- 2023/11/23 06:42 [medline]
PHST- 2023/09/12 00:42 [pubmed]
PHST- 2023/09/11 19:18 [entrez]
AID - S0141-8130(23)03638-3 [pii]
AID - 10.1016/j.ijbiomac.2023.126741 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2023 Dec 31;253(Pt 2):126741. doi: 
      10.1016/j.ijbiomac.2023.126741. Epub 2023 Sep 9.