PMID- 37700733 OWN - NLM STAT- MEDLINE DCOM- 20230914 LR - 20231004 IS - 2038-8306 (Electronic) IS - 1121-760X (Print) IS - 1121-760X (Linking) VI - 67 IP - 3 DP - 2023 Sep 12 TI - Crocin exerts anti-tumor effect in colon cancer cells via repressing the JAK pathway. LID - 10.4081/ejh.2023.3697 [doi] LID - 3697 AB - Crocin has been reported to have therapeutic effects on multiple cancers including colon cancer, but its specific mechanism is still ambiguous and needs to be further explored. Human colorectal adenocarcinoma cells (HCT-116) and human normal colonic epithelial cells (CCD841) were first treated with increasing concentrations of crocin. Subsequently, with 150 and 200 muM of crocin, the cell vitality was examined by cell counting kit 8. Cell apoptosis and proliferation were tested by TUNEL staining and colony formation assay, respectively. The expression of Ki-67 was assessed by immunofluorescence. Enzyme-linked immunosorbent assay was used to evaluate the level of inflammation- and oxidative-related factors. The reactive oxygen species (ROS) production and mitochondrial membrane potential (MMP) were examined by flow cytometer. Janus kinase (JAK), signal transducer and activator of transcription 3 (STAT3), and extracellular regulated protein kinases (ERK) in HCT-116 cells were tested by Western blot. Different concentrations of crocin barely affected the CCD841 cell vitality, while crocin restrained the HCT-116 cells vitality, proliferation and the expression of Ki-67, while inducing apoptosis in a concentration-dependent manner. Moreover, the contents of inflammation- and oxidative-related factors in HCT-116 cells were largely blunted by crocin that enhanced ROS and restrained the MMP and suppressed p-JAK2/JAK2, p-STAT3/STAT3, and p-ERK/ERK expression in HCT-116 cells. Crocin induced apoptosis and restored mitochondrial function in HCT-116 cells via repressing the JAK pathway. If the threptic effect works in patients, it could herald a new, effective treatment for colon cancer, improving the patients' prognosis and quality of life. FAU - Yang, Hui AU - Yang H AD - Department of Gastroenterology, Changxing People's Hospital, Huzhou, Zhejiang. Yanghuikeyan54@163.com. FAU - Zhang, Yunlong AU - Zhang Y AD - Department of Ultrasound, The First People's Hospital of Linping District, Hangzhou, Zhejiang. 397062405@qq.com. FAU - Zhang, Desheng AU - Zhang D AD - Department of Radiology, Affiliated Center Hospital of Huzhou University, Huzhou, Zhejiang. mayomedical1@163.com. FAU - Qian, Liping AU - Qian L AD - Department of Radiology, Hangzhou Cancer Hospital, Hangzhou, Zhejiang. nolan761@163.com. FAU - Yang, Tianxing AU - Yang T AD - Department of Oncology, Sanmen County People's Hospital, Taizhou, Zhejiang. smcrab@126.com. FAU - Wu, Xiaocheng AU - Wu X AD - Pathology Laboratory, Hangzhou Dean Medical Laboratory, Hangzhou, Zhejiang. wuxc1984@163.com. LA - eng PT - Journal Article DEP - 20230912 PL - Italy TA - Eur J Histochem JT - European journal of histochemistry : EJH JID - 9207930 RN - 877GWI46C2 (crocin) RN - EC 2.7.10.2 (Janus Kinases) RN - 0 (Ki-67 Antigen) RN - 0 (Reactive Oxygen Species) SB - IM MH - Humans MH - *Janus Kinases MH - Ki-67 Antigen MH - Quality of Life MH - Reactive Oxygen Species MH - *Colonic Neoplasms/drug therapy PMC - PMC10543190 EDAT- 2023/09/13 06:42 MHDA- 2023/09/14 06:43 PMCR- 2023/09/12 CRDT- 2023/09/13 03:53 PHST- 2023/02/27 00:00 [received] PHST- 2023/08/10 00:00 [accepted] PHST- 2023/09/14 06:43 [medline] PHST- 2023/09/13 06:42 [pubmed] PHST- 2023/09/13 03:53 [entrez] PHST- 2023/09/12 00:00 [pmc-release] AID - 10.4081/ejh.2023.3697 [doi] PST - epublish SO - Eur J Histochem. 2023 Sep 12;67(3):3697. doi: 10.4081/ejh.2023.3697.