PMID- 37700922
OWN - NLM
STAT- MEDLINE
DCOM- 20230914
LR  - 20230914
IS  - 1986-5961 (Electronic)
IS  - 0350-199X (Print)
IS  - 0350-199X (Linking)
VI  - 77
IP  - 3
DP  - 2023
TI  - TGF-beta1 Effects on Total Collagen of the New Zealand Rabbit's Urethral Wall 
      (Oryctolagus cuniculus) in Animal Models of Urethral Stricture.
PG  - 189-193
LID - 10.5455/medarh.2023.77.189-193 [doi]
AB  - BACKGROUND: Currently, animal models of urethral stricture are not standardized. 
      Transforming Growth Factor Beta 1 (TGF-beta1) regulates extracellular matrix 
      deposition in homeostatic and pathological responses. OBJECTIVE: The aim of this 
      study was to present the potential model to be developed as a urethral stricture. 
      METHODS: True experimental laboratory research was conducted by using Male New 
      Zealand rabbits (Oryctolagus cuniculus), which were divided into 5 groups; 
      control, placebo, and 3 treatment groups (TGF-beta1 injection of 1 microg, 2 microg, 4 microg). 
      Urethrography, histopathological analysis, and evaluation of total collagen 
      formation of the urethral wall were performed after 6 weeks. RESULTS: An increase 
      in the dose of TGF-beta1 decreased the mean rabbit's urethral lumen diameter (29.3% 
      in the 2microg group and 34% in the 4microg group) compared to controls. Three rabbits 
      decreased as much as </= 50% in urethral lumen diameter. Significant increases in 
      total collagen density in the periluminal and peripheral urethral spongiosum were 
      noted by increasing doses of TGF-beta1. The percentage of urethral lumen diameter 
      has a strong negative correlation with periluminal total collagen density (r = 
      -0,798; p = 0,000) and very strong negative correlation with peripheral spongiosa 
      total collagen density (r = -0,748, p = 0,000). CONCLUSION: TGF-beta1 plays a role 
      in changing total collagen compositions of the rabbit's urethral wall, decreasing 
      the urethral lumen diameter. Further research with increasing doses of TGF-beta1 is 
      needed to determine the effective dose of TGF-beta1 in inducing urethral stricture.
CI  - (c) 2023 Paksi Satyagraha, Hamid Hunaif Dhofi Alluza,I Made Udiyana Indradiputra, 
      Athaya Febriantyo Purnomo, Pradana Nurhadi, Kenty Wantri Anita, Yuyun Yueniwati, 
      Happy Kurnia Permatasari, Basuki Bambang Purnomo.
FAU - Satyagraha, Paksi
AU  - Satyagraha P
AD  - Doctoral Program in Medical Science, Faculty of Medicine Universitas Brawijaya, 
      Malang, Indonesia.
AD  - Department of Urology, Faculty of Medicine Universitas Brawijaya - Saiful Anwar 
      General Hospital, Malang, Indonesia.
FAU - Alluza, Hamid Hunaif Dhofi
AU  - Alluza HHD
AD  - Department of Urology, Faculty of Medicine Universitas Brawijaya - Saiful Anwar 
      General Hospital, Malang, Indonesia.
FAU - Indradiputra, I Made Udiyana
AU  - Indradiputra IMU
AD  - Department of Urology, Faculty of Medicine Universitas Brawijaya - Saiful Anwar 
      General Hospital, Malang, Indonesia.
FAU - Purnomo, Athaya Febriantyo
AU  - Purnomo AF
AD  - Department of Urology, Faculty of Medicine Universitas Brawijaya - Saiful Anwar 
      General Hospital, Malang, Indonesia.
FAU - Nurhadi, Pradana
AU  - Nurhadi P
AD  - Department of Urology, Faculty of Medicine Universitas Brawijaya - Saiful Anwar 
      General Hospital, Malang, Indonesia.
FAU - Anita, Kenty Wantri
AU  - Anita KW
AD  - Department of Pathology Anatomy, Faculty of Medicine Universitas Brawijaya - 
      Saiful Anwar General Hospital, Malang, Indonesia.
FAU - Yueniwati, Yuyun
AU  - Yueniwati Y
AD  - Department of Radiology, Faculty of Medicine Universitas Brawijaya - Saiful Anwar 
      General Hospital, Malang, Indonesia.
FAU - Permatasari, Happy Kurnia
AU  - Permatasari HK
AD  - Department of Biochemistry, Faculty of Medicine, Universitas Brawijaya, Malang, 
      Indonesia.
FAU - Purnomo, Basuki Bambang
AU  - Purnomo BB
AD  - Department of Urology, Faculty of Medicine Universitas Brawijaya - Saiful Anwar 
      General Hospital, Malang, Indonesia.
LA  - eng
PT  - Journal Article
PL  - Bosnia and Herzegovina
TA  - Med Arch
JT  - Medical archives (Sarajevo, Bosnia and Herzegovina)
JID - 101635337
RN  - 0 (Transforming Growth Factor beta1)
RN  - 9007-34-5 (Collagen)
RN  - 26GIW6ZLPH (rimiterol)
SB  - IM
MH  - Male
MH  - Rabbits
MH  - Animals
MH  - *Urethral Stricture
MH  - Transforming Growth Factor beta1
MH  - Urethra
MH  - Collagen
MH  - Models, Animal
PMC - PMC10495137
OTO - NOTNLM
OT  - TGF-beta1
OT  - Total Collagen
OT  - Urethral Stricture
EDAT- 2023/09/13 06:41
MHDA- 2023/09/14 06:43
PMCR- 2023/01/01
CRDT- 2023/09/13 03:58
PHST- 2023/04/06 00:00 [received]
PHST- 2023/05/10 00:00 [accepted]
PHST- 2023/09/14 06:43 [medline]
PHST- 2023/09/13 06:41 [pubmed]
PHST- 2023/09/13 03:58 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 10.5455/medarh.2023.77.189-193 [doi]
PST - ppublish
SO  - Med Arch. 2023;77(3):189-193. doi: 10.5455/medarh.2023.77.189-193.