PMID- 3770103
OWN - NLM
STAT- MEDLINE
DCOM- 19861210
LR  - 20131121
IS  - 0301-472X (Print)
IS  - 0301-472X (Linking)
VI  - 14
IP  - 10
DP  - 1986 Nov
TI  - Countercurrent centrifugal elutriation (CCE) recovery profiles of hematopoietic 
      stem cells in marrow from normal and 5-FU-treated mice.
PG  - 963-70
AB  - Data presented in this report describe countercurrent centrifugal elutriation 
      (CCE) recovery profiles of hematopoietic colony-forming cells (CFC) in marrow 
      from normal and 5-fluorouracil-(5-FU) treated mice. Of the total nucleated cells, 
      75%-95% were recovered, and up to 80% of CFC were recovered after CCE of bone 
      marrow from normal mice. Red blood cells and the majority of lymphocytes were 
      collected in fractions well separated from the CFC. In addition, the CCE recovery 
      profiles of populations of CFC (i.e., BFU-E, CFU-E, GM-CFC, and HPP-CFC) were 
      distinct. The distribution of recovered day 8 CFU-S was different from the 
      distribution of day 12 CFU-S. The CCE recovery profiles of CFC in regenerating 
      marrow from 5-FU-treated mice were shifted to fractions of larger cells, 
      presumably in cell cycle. These data demonstrate that CCE is useful as a method 
      of further characterizing qualitative and quantitative changes in populations of 
      CFC occurring after various hematopoietic-influencing regimens.
FAU - Schwartz, G N
AU  - Schwartz GN
FAU - MacVittie, T J
AU  - MacVittie TJ
FAU - Monroy, R L
AU  - Monroy RL
FAU - Vigneulle, R M
AU  - Vigneulle RM
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Exp Hematol
JT  - Experimental hematology
JID - 0402313
RN  - U3P01618RT (Fluorouracil)
SB  - IM
MH  - Animals
MH  - Bone Marrow/drug effects
MH  - *Bone Marrow Cells
MH  - Cell Separation/*methods
MH  - Countercurrent Distribution
MH  - Female
MH  - Fluorouracil/*pharmacology
MH  - Granulocytes
MH  - Hematopoietic Stem Cells/classification/*cytology
MH  - Macrophages
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred DBA
EDAT- 1986/11/01 00:00
MHDA- 1986/11/01 00:01
CRDT- 1986/11/01 00:00
PHST- 1986/11/01 00:00 [pubmed]
PHST- 1986/11/01 00:01 [medline]
PHST- 1986/11/01 00:00 [entrez]
PST - ppublish
SO  - Exp Hematol. 1986 Nov;14(10):963-70.