PMID- 37706337 OWN - NLM STAT- MEDLINE DCOM- 20231205 LR - 20240415 IS - 1552-4965 (Electronic) IS - 1549-3296 (Linking) VI - 112 IP - 2 DP - 2024 Feb TI - The role of plasma-induced surface chemistry on polycaprolactone nanofibers to direct chondrogenic differentiation of human mesenchymal stem cells. PG - 210-230 LID - 10.1002/jbm.a.37607 [doi] AB - Bone marrow-derived mesenchymal stromal cells (BMSCs) are extensively being utilized for cartilage regeneration owing to their excellent differentiation potential and availability. However, controlled differentiation of BMSCs towards cartilaginous phenotypes to heal full-thickness cartilage defects remains challenging. This study investigates how different surface properties induced by either coating deposition or biomolecules immobilization onto nanofibers (NFs) could affect BMSCs chondro-inductive behavior. Accordingly, electrospun poly(epsilon-caprolactone) (PCL) NFs were exposed to two surface modification strategies based on medium-pressure plasma technology. The first strategy is plasma polymerization, in which cyclopropylamine (CPA) or acrylic acid (AcAc) monomers were plasma polymerized to obtain amine- or carboxylic acid-rich NFs, respectively. The second strategy uses a combination of CPA plasma polymerization and a post-chemical technique to immobilize chondroitin sulfate (CS) onto the NFs. These modifications could affect surface roughness, hydrophilicity, and chemical composition while preserving the NFs' nano-morphology. The results of long-term BMSCs culture in both basic and chondrogenic media proved that the surface modifications modulated BMSCs chondrogenic differentiation. Indeed, the incorporation of polar groups by different modification strategies had a positive impact on the cell proliferation rate, production of the glycosaminoglycan matrix, and expression of extracellular matrix proteins (collagen I and collagen II). The chondro-inductive behavior of the samples was highly dependent on the nature of the introduced polar functional groups. Among all samples, carboxylic acid-rich NFs promoted chondrogenesis by higher expression of aggrecan, Sox9, and collagen II with downregulation of hypertrophic markers. Hence, this approach showed an intrinsic potential to have a non-hypertrophic chondrogenic cell phenotype. CI - (c) 2023 The Authors. Journal of Biomedical Materials Research Part A published by Wiley Periodicals LLC. FAU - Asadian, Mahtab AU - Asadian M AD - Research Unit Plasma Technology (RUPT), Department of Applied Physics, Ghent University, Ghent, Belgium. AD - Prometheus Division of Skeletal Tissue Engineering, Department of Materials Science, KU Leuven University, Leuven, Belgium. FAU - Tomasina, Clarissa AU - Tomasina C AUID- ORCID: 0000-0002-5100-3725 AD - MERLN Institute for Technology-Inspired Regenerative Medicine, Complex Tissue Regeneration Department, Maastricht University, Maastricht, The Netherlands. FAU - Onyshchenko, Yuliia AU - Onyshchenko Y AD - Research Unit Plasma Technology (RUPT), Department of Applied Physics, Ghent University, Ghent, Belgium. FAU - Chan, Ke Vin AU - Chan KV AD - Research Unit Plasma Technology (RUPT), Department of Applied Physics, Ghent University, Ghent, Belgium. FAU - Norouzi, Mohammad AU - Norouzi M AD - Department of Pharmacology, University of Montreal, Montreal, Quebec, Canada. FAU - Zonderland, Jip AU - Zonderland J AD - MERLN Institute for Technology-Inspired Regenerative Medicine, Complex Tissue Regeneration Department, Maastricht University, Maastricht, The Netherlands. FAU - Camarero-Espinosa, Sandra AU - Camarero-Espinosa S AD - MERLN Institute for Technology-Inspired Regenerative Medicine, Complex Tissue Regeneration Department, Maastricht University, Maastricht, The Netherlands. AD - POLYMAT University of the Basque Country UPV/EHU Avenida Tolosa 72, Donostia/San Sebastian, Spain. AD - IKERBASQUE, Basque Foundation for Science, Euskadi Pl. 5, Bilbao, Spain. FAU - Morent, Rino AU - Morent R AD - Research Unit Plasma Technology (RUPT), Department of Applied Physics, Ghent University, Ghent, Belgium. FAU - De Geyter, Nathalie AU - De Geyter N AD - Research Unit Plasma Technology (RUPT), Department of Applied Physics, Ghent University, Ghent, Belgium. FAU - Moroni, Lorenzo AU - Moroni L AD - MERLN Institute for Technology-Inspired Regenerative Medicine, Complex Tissue Regeneration Department, Maastricht University, Maastricht, The Netherlands. LA - eng GR - 1217820N/Fonds Wetenschappelijk Onderzoek/ GR - V419919N/Fonds Wetenschappelijk Onderzoek/ GR - 814410/Horizon 2020 Framework Programme/ PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20230914 PL - United States TA - J Biomed Mater Res A JT - Journal of biomedical materials research. Part A JID - 101234237 RN - 24980-41-4 (polycaprolactone) RN - 9007-34-5 (Collagen) RN - 0 (Carboxylic Acids) SB - IM MH - Humans MH - Chondrogenesis MH - *Nanofibers MH - Cell Differentiation MH - Collagen/chemistry MH - *Mesenchymal Stem Cells MH - Carboxylic Acids MH - Cells, Cultured OTO - NOTNLM OT - biomolecules immobilization OT - bone marrow-derived mesenchymal stromal cells OT - cell differentiation OT - electrospinning OT - plasma modification OT - plasma polymerization EDAT- 2023/09/14 06:42 MHDA- 2023/12/05 12:43 CRDT- 2023/09/14 05:34 PHST- 2023/08/12 00:00 [revised] PHST- 2023/03/27 00:00 [received] PHST- 2023/08/24 00:00 [accepted] PHST- 2023/12/05 12:43 [medline] PHST- 2023/09/14 06:42 [pubmed] PHST- 2023/09/14 05:34 [entrez] AID - 10.1002/jbm.a.37607 [doi] PST - ppublish SO - J Biomed Mater Res A. 2024 Feb;112(2):210-230. doi: 10.1002/jbm.a.37607. Epub 2023 Sep 14.