PMID- 37706441 OWN - NLM STAT- MEDLINE DCOM- 20231102 LR - 20231108 IS - 1555-3892 (Electronic) IS - 0963-6897 (Print) IS - 0963-6897 (Linking) VI - 32 DP - 2023 Jan-Dec TI - Role of Serum Inflammatory Cytokines in Sepsis Rats Following BMSCs Transplantation: Protein Microarray Analysis. PG - 9636897231198175 LID - 10.1177/09636897231198175 [doi] LID - 09636897231198175 AB - Bone marrow stromal cells (BMSCs) have emerged as a potential therapy for sepsis, yet the underlying mechanisms remain unclear. In this study, we investigated the effects of BMSCs on serum inflammatory cytokines in a rat model of lipopolysaccharide (LPS)-induced sepsis. Sepsis was induced by intravenous injection of LPS, followed by transplantation of BMSCs. We monitored survival rates for 72 h and evaluated organ functions, histopathological changes, and cytokines expression. Sepsis rats showed decreased levels of white blood cells, platelets, lymphocyte ratio, and oxygen partial pressure, along with increased levels of neutrophil ratio, carbon dioxide partial pressure, lactic acid, alanine aminotransferase, and aspartate aminotransferase. Histologically, lung, intestine, and liver tissues exhibited congestion, edema, and infiltration of inflammatory cells. However, after BMSCs treatment, there was improvement in organ functions, histopathological injuries, and survival rates. Protein microarray analysis revealed significant changes in the expression of 12 out of 34 inflammatory cytokines. These findings were confirmed by enzyme-linked immunosorbent assay. Pro-inflammatory factors, such as interleukin-1beta (IL-1beta), IL-1alpha, tumor necrosis factor-alpha (TNF-alpha), tissue inhibitor of metal protease 1 (TIMP-1), matrix metalloproteinase 8 (MMP-8), Leptin, and L-selectin were upregulated in sepsis, whereas anti-inflammatory and growth factors, including IL-4, beta-nerve growth factor (beta-NGF), ciliary neurotrophic factor (CNTF), interferon gamma (IFN-gamma), and Activin A were downregulated. BMSCs transplantation led to a decrease in pro-inflammatory cytokines and an increase in anti-inflammatory and growth factors. We summarized relevant molecular signaling pathways that resulted from cytokines in BMSCs for treating sepsis. Our results illustrated that BMSCs could promote tissue repair and improve organ functions and survival rates in sepsis through modulating cytokine networks. FAU - Xiu, Guanghui AU - Xiu G AUID- ORCID: 0000-0001-7734-3843 AD - Affiliated Hospital of Yunnan University (The Second People's Hospital of Yunnan Province), School of Medicine, Yunnan University, Kunming, China. FAU - Li, Xiuling AU - Li X AD - Department of Obstetrics, The First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, China. FAU - Chen, Juan AU - Chen J AD - Department of Infectious Diseases, The First People's Hospital of Fuzhou, Fuzhou, China. FAU - Li, Jintao AU - Li J AD - Institute of Neuroscience, Kunming Medicine University, Kunming, China. FAU - Chen, Kun AU - Chen K AD - The Third Clinical School of Medicine, Jinzhou Medical University, Jinzhou, China. FAU - Liu, Ping AU - Liu P AD - Affiliated Hospital of Yunnan University (The Second People's Hospital of Yunnan Province), School of Medicine, Yunnan University, Kunming, China. FAU - Ling, Bin AU - Ling B AD - Affiliated Hospital of Yunnan University (The Second People's Hospital of Yunnan Province), School of Medicine, Yunnan University, Kunming, China. FAU - Yang, Ying AU - Yang Y AD - Affiliated Hospital of Yunnan University (The Second People's Hospital of Yunnan Province), School of Medicine, Yunnan University, Kunming, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Cell Transplant JT - Cell transplantation JID - 9208854 RN - 0 (Cytokines) RN - 0 (Lipopolysaccharides) RN - 0 (Tumor Necrosis Factor-alpha) RN - 0 (Anti-Inflammatory Agents) SB - IM MH - Rats MH - Animals MH - Cytokines/metabolism MH - Lipopolysaccharides/pharmacology MH - Protein Array Analysis MH - Tumor Necrosis Factor-alpha/metabolism MH - Inflammation/metabolism MH - Anti-Inflammatory Agents/therapeutic use MH - *Mesenchymal Stem Cells/metabolism MH - *Sepsis/therapy PMC - PMC10503277 OTO - NOTNLM OT - bone marrow stromal cells OT - inflammatory cytokines OT - protein array analysis OT - sepsis COIS- The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. EDAT- 2023/09/14 12:41 MHDA- 2023/09/14 12:42 PMCR- 2023/09/14 CRDT- 2023/09/14 08:22 PHST- 2023/09/14 12:42 [medline] PHST- 2023/09/14 12:41 [pubmed] PHST- 2023/09/14 08:22 [entrez] PHST- 2023/09/14 00:00 [pmc-release] AID - 10.1177_09636897231198175 [pii] AID - 10.1177/09636897231198175 [doi] PST - ppublish SO - Cell Transplant. 2023 Jan-Dec;32:9636897231198175. doi: 10.1177/09636897231198175.