PMID- 37708022
OWN - NLM
STAT- Publisher
LR  - 20231004
IS  - 2211-1247 (Electronic)
VI  - 42
IP  - 9
DP  - 2023 Sep 26
TI  - Organization of Purkinje cell development by neuronal MEGF11 in cerebellar 
      granule cells.
PG  - 113137
LID - S2211-1247(23)01149-X [pii]
LID - 10.1016/j.celrep.2023.113137 [doi]
AB  - As cerebellar granule cells (GCs) coordinate the formation of regular cerebellar 
      networks during postnatal development, molecules in GCs are expected to be 
      involved. Here, we test the effects of the knockdown (KD) of multiple epidermal 
      growth factor-like domains protein 11 (MEGF11), which is a homolog of proteins 
      mediating astrocytic phagocytosis but is substantially increased at the later 
      developmental stages of GCs on cerebellar development. MEGF11-KD in GCs of 
      developing mice results in abnormal cerebellar structures, including extensively 
      ectopic Purkinje cell (PC) somas, and in impaired motor functions. MEGF11-KD also 
      causes abnormally asynchronous synaptic release from GC axons, parallel fibers, 
      before the appearance of abnormal cerebellar structures. Interestingly, blockade 
      of this abnormal synaptic release restores most of the cerebellar structures. 
      Thus, apart from phagocytic functions of its related homologs in astrocytes, 
      MEGF11 in GCs promotes proper PC development and cerebellar network formation by 
      regulating immature synaptic transmission.
CI  - Copyright (c) 2023 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Jun, Soyoung
AU  - Jun S
AD  - Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 
      02792, Republic of Korea; Division of Bio-Medical Science and Technology, KIST 
      School, Korea University of Science and Technology (UST), Seoul 02792, Republic 
      of Korea.
FAU - Kim, Muwoong
AU  - Kim M
AD  - Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 
      02792, Republic of Korea; Division of Bio-Medical Science and Technology, KIST 
      School, Korea University of Science and Technology (UST), Seoul 02792, Republic 
      of Korea.
FAU - Park, Heeyoun
AU  - Park H
AD  - Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 
      02792, Republic of Korea.
FAU - Hwang, Eunmi
AU  - Hwang E
AD  - Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 
      02792, Republic of Korea; Division of Bio-Medical Science and Technology, KIST 
      School, Korea University of Science and Technology (UST), Seoul 02792, Republic 
      of Korea.
FAU - Yamamoto, Yukio
AU  - Yamamoto Y
AD  - Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 
      02792, Republic of Korea. Electronic address: yukio.kist@gmail.com.
FAU - Tanaka-Yamamoto, Keiko
AU  - Tanaka-Yamamoto K
AD  - Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 
      02792, Republic of Korea; Division of Bio-Medical Science and Technology, KIST 
      School, Korea University of Science and Technology (UST), Seoul 02792, Republic 
      of Korea. Electronic address: keikoyamat@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20230913
PL  - United States
TA  - Cell Rep
JT  - Cell reports
JID - 101573691
SB  - IM
OTO - NOTNLM
OT  - CP: Neuroscience
OT  - MEGF11
OT  - Purkinje cell soma alignment
OT  - cerebellar granule cell
OT  - climbing fiber synapse formation
OT  - migration
OT  - motor coordination
OT  - shRNA-mediated knockdown
OT  - synchronous neurotransmitter release
COIS- Declaration of interests The authors declare no competing interests.
EDAT- 2023/09/14 18:43
MHDA- 2023/09/14 18:43
CRDT- 2023/09/14 12:35
PHST- 2023/02/14 00:00 [received]
PHST- 2023/07/24 00:00 [revised]
PHST- 2023/08/31 00:00 [accepted]
PHST- 2023/09/14 18:43 [pubmed]
PHST- 2023/09/14 18:43 [medline]
PHST- 2023/09/14 12:35 [entrez]
AID - S2211-1247(23)01149-X [pii]
AID - 10.1016/j.celrep.2023.113137 [doi]
PST - ppublish
SO  - Cell Rep. 2023 Sep 26;42(9):113137. doi: 10.1016/j.celrep.2023.113137. Epub 2023 
      Sep 13.