PMID- 37709188
OWN - NLM
STAT- MEDLINE
DCOM- 20240221
LR  - 20240221
IS  - 1638-6183 (Electronic)
IS  - 0300-9084 (Linking)
VI  - 218
DP  - 2024 Mar
TI  - Structure of glyceraldehyde-3-phosphate dehydrogenase from Paracoccidioides 
      lutzii in complex with an aldonic sugar acid.
PG  - 20-33
LID - S0300-9084(23)00240-7 [pii]
LID - 10.1016/j.biochi.2023.09.013 [doi]
AB  - The pathogen Paracoccidioides lutzii (Pb01) is found in South America countries 
      Colombia, Ecuador, Venezuela and Brazil, especially in the central, west, and 
      north regions of the latter. It belongs to the Ajellomycetaceae family, 
      Onygenales order, and is typically thermodimorphic, presenting yeast cells when 
      it grows in animal tissues, but mycelia when in the environment, where it 
      produces the infectious propagule. This fungus is one of the etiologic agents of 
      Paracoccidioidomycosis (PCM), the most important endemic fungal infection in 
      Latin America. Investigations on its genome have contributed to a better 
      understanding about its metabolism and revealed the complexity of several 
      metabolic glycolytic pathways. Glyceraldehyde-3-Phosphate Dehydrogenase from 
      Paracoccidioides lutzii (PlGAPDH) is considered a moonlighting protein and 
      participates in several biological processes of this pathogen. The enzyme was 
      expressed and purified, as seen in SDS-PAGE gel, crystallized and had its three 
      dimensional structure (3D) determined in complex with NAD(+), a sulphate ion and 
      d-galactonic acid, therefore, a type of 'GAA site'. It is the first GAPDH 
      structure to show this chemical type in this site and how this protein can bind 
      an acid derived from oxidation of a linear hexose.
CI  - Copyright (c) 2023 Elsevier B.V. and Societe Francaise de Biochimie et Biologie 
      Moleculaire (SFBBM). All rights reserved.
FAU - Hernandez-Prieto, Jonathan Heiler
AU  - Hernandez-Prieto JH
AD  - Department of Chemistry, State University of Ponta Grossa, Ponta Grossa, PR, 
      84030-900, Brazil.
FAU - Martini, Viviane Paula
AU  - Martini VP
AD  - Federal Institute of Parana-Campus Irati, Irati, PR, 84507-302, Brazil.
FAU - Iulek, Jorge
AU  - Iulek J
AD  - Department of Chemistry, State University of Ponta Grossa, Ponta Grossa, PR, 
      84030-900, Brazil. Electronic address: iulek@uepg.br.
LA  - eng
PT  - Journal Article
DEP - 20230912
PL  - France
TA  - Biochimie
JT  - Biochimie
JID - 1264604
RN  - 0 (Sugars)
RN  - Paracoccidioides lutzii
SB  - IM
MH  - Animals
MH  - *Paracoccidioides/genetics
MH  - *Paracoccidioidomycosis/epidemiology/microbiology
MH  - Brazil/epidemiology
MH  - Sugars
OTO - NOTNLM
OT  - GAA site
OT  - Glyceraldehyde-3-phosphate dehydrogenase
OT  - Paracoccidioides lutzii
OT  - Paracoccidioidomycosis
OT  - d-galactonic acid
COIS- Declaration of competing interest We have no conflict of interest to declare.
EDAT- 2023/09/15 00:42
MHDA- 2024/02/21 11:15
CRDT- 2023/09/14 19:18
PHST- 2023/02/19 00:00 [received]
PHST- 2023/07/23 00:00 [revised]
PHST- 2023/09/11 00:00 [accepted]
PHST- 2024/02/21 11:15 [medline]
PHST- 2023/09/15 00:42 [pubmed]
PHST- 2023/09/14 19:18 [entrez]
AID - S0300-9084(23)00240-7 [pii]
AID - 10.1016/j.biochi.2023.09.013 [doi]
PST - ppublish
SO  - Biochimie. 2024 Mar;218:20-33. doi: 10.1016/j.biochi.2023.09.013. Epub 2023 Sep 
      12.