PMID- 37709460
OWN - NLM
STAT- MEDLINE
DCOM- 20230918
LR  - 20230918
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 1278
DP  - 2023 Oct 16
TI  - Rapid and sensitive determination of free fatty acids based on in-source 
      microdroplet-driven derivatization coupled with high-resolution mass 
      spectrometry.
PG  - 341717
LID - S0003-2670(23)00938-8 [pii]
LID - 10.1016/j.aca.2023.341717 [doi]
AB  - Accurate and sensitive measurements of free fatty acids (FFAs) in biological 
      samples are valuable for diagnosing and prognosing diseases. In this study, an 
      in-source microdroplet derivation strategy combined with high-resolution mass 
      spectrometry was developed to analyze FFAs in lipid extracts of biological 
      samples directly. FFAs were rapidly derivated with 2-picolylamine (PA) in the 
      microdroplet which is derived by electrospray. With the proposed method, twelve 
      typical FFAs were determined reliably with high sensitivity and acceptable 
      linearities (R(2) >/= 0.94). The LODs and LOQs for the twelve FFAs were 
      9-76 pg mL(-1) and 30-253 pg mL(-1), respectively. The developed method was 
      applied to analyze the alteration of FFAs in liver and kidney samples of rats 
      induced by perfluorooctane sulfonate (PFOS) exposure. The good results 
      demonstrate that the established analysis technique is dependable and has 
      promising applications in detecting FFAs associated with complex biological 
      samples.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Cui, Jiaqi
AU  - Cui J
AD  - Flavor Research Center, Zhengzhou University, Zhengzhou, 450001, China.
FAU - Zhang, Jianxun
AU  - Zhang J
AD  - Flavor Research Center, Zhengzhou University, Zhengzhou, 450001, China; The Key 
      Laboratory of Tobacco Flavor Basic Research of CNTC, Zhengzhou Tobacco Research 
      Institute, Zhengzhou, 450001, China.
FAU - Sun, Shihao
AU  - Sun S
AD  - Flavor Research Center, Zhengzhou University, Zhengzhou, 450001, China; The Key 
      Laboratory of Tobacco Flavor Basic Research of CNTC, Zhengzhou Tobacco Research 
      Institute, Zhengzhou, 450001, China.
FAU - Fan, Wu
AU  - Fan W
AD  - Flavor Research Center, Zhengzhou University, Zhengzhou, 450001, China; The Key 
      Laboratory of Tobacco Flavor Basic Research of CNTC, Zhengzhou Tobacco Research 
      Institute, Zhengzhou, 450001, China.
FAU - Xi, Hui
AU  - Xi H
AD  - The Key Laboratory of Tobacco Flavor Basic Research of CNTC, Zhengzhou Tobacco 
      Research Institute, Zhengzhou, 450001, China.
FAU - Xu, Xiujuan
AU  - Xu X
AD  - The Key Laboratory of Tobacco Flavor Basic Research of CNTC, Zhengzhou Tobacco 
      Research Institute, Zhengzhou, 450001, China.
FAU - Ji, Lingbo
AU  - Ji L
AD  - The Key Laboratory of Tobacco Flavor Basic Research of CNTC, Zhengzhou Tobacco 
      Research Institute, Zhengzhou, 450001, China.
FAU - Zhang, Shusheng
AU  - Zhang S
AD  - Flavor Research Center, Zhengzhou University, Zhengzhou, 450001, China; Center of 
      Advanced Analysis and Gene Sequencing, Zhengzhou University, Zhengzhou, 450001, 
      China.
FAU - Wang, Dingzhong
AU  - Wang D
AD  - Flavor Research Center, Zhengzhou University, Zhengzhou, 450001, China; The Key 
      Laboratory of Tobacco Flavor Basic Research of CNTC, Zhengzhou Tobacco Research 
      Institute, Zhengzhou, 450001, China. Electronic address: wdz426@163.com.
FAU - Zhao, Wuduo
AU  - Zhao W
AD  - Flavor Research Center, Zhengzhou University, Zhengzhou, 450001, China; Center of 
      Advanced Analysis and Gene Sequencing, Zhengzhou University, Zhengzhou, 450001, 
      China. Electronic address: zhaowuduo@163.com.
LA  - eng
PT  - Journal Article
DEP - 20230815
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Fatty Acids, Nonesterified)
SB  - IM
MH  - Animals
MH  - Rats
MH  - *Fatty Acids, Nonesterified
MH  - *Kidney
MH  - Limit of Detection
MH  - Liver
MH  - Mass Spectrometry
OTO - NOTNLM
OT  - Free fatty acids (FFAs)
OT  - High-resolution mass spectrometry (HRMS)
OT  - In-source microdroplet-driven derivatization
OT  - Perfluorooctane sulfonate (PFOS) exposure
OT  - Rapid determination
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/09/15 00:42
MHDA- 2023/09/18 12:44
CRDT- 2023/09/14 21:00
PHST- 2023/04/21 00:00 [received]
PHST- 2023/07/16 00:00 [revised]
PHST- 2023/08/13 00:00 [accepted]
PHST- 2023/09/18 12:44 [medline]
PHST- 2023/09/15 00:42 [pubmed]
PHST- 2023/09/14 21:00 [entrez]
AID - S0003-2670(23)00938-8 [pii]
AID - 10.1016/j.aca.2023.341717 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2023 Oct 16;1278:341717. doi: 10.1016/j.aca.2023.341717. Epub 
      2023 Aug 15.