PMID- 37716172
OWN - NLM
STAT- MEDLINE
DCOM- 20231009
LR  - 20231018
IS  - 1095-8630 (Electronic)
IS  - 0301-4797 (Linking)
VI  - 346
DP  - 2023 Nov 15
TI  - Enhanced stability and catalytic performance of laccase immobilized on magnetic 
      graphene oxide modified with ionic liquids.
PG  - 118975
LID - S0301-4797(23)01763-2 [pii]
LID - 10.1016/j.jenvman.2023.118975 [doi]
AB  - Graphite oxide (GO) is an excellent laccase immobilization material. However, the 
      electrostatic interaction between graphene leads to the accumulation of GO, as 
      well as the interaction with the surface of enzyme molecules causing protein 
      denaturation and deactivation, which limits its further industrial application. 
      In this study, the ionic liquids (ILs) modification strategy was proposed to 
      improve the stability and catalytic performance of immobilized laccase. The 
      laccase-ILs-MGO exhibited remarkable enzymatic properties, with significant 
      enhancements in organic solvent tolerance, thermal and operational stability. The 
      laccase-ILs-MGO system exhibited a remarkable removal efficiency of 95.5% towards 
      2,4-dichlorophenol (2,4-DCP) within 12 h and maintained over 70.0% removal 
      efficiency after seven reaction cycles. In addition, the efficient elimination of 
      other phenolic compounds and recalcitrant polycyclic aromatic hydrocarbons could 
      also be accomplished. Molecular dynamics simulation and molecular docking studies 
      demonstrated that immobilized laccase exhibited superior structural rigidity and 
      stronger hydrogen bond interactions with substrates compared to free laccase, 
      which was beneficial for the stability of both the laccase and substrate 
      degradation efficiency. Therefore, this study proposed a simple and practical 
      strategy for modifying GO with ILs, providing novel insights into developing 
      efficient enzyme immobilization techniques.
CI  - Copyright (c) 2023 Elsevier Ltd. All rights reserved.
FAU - Zhang, Wei
AU  - Zhang W
AD  - State Key Laboratory of Materials-Oriented Chemical Engineering, School of 
      Pharmaceutical Sciences, Nanjing Tech University, Nanjing, China.
FAU - Zhang, Yifei
AU  - Zhang Y
AD  - State Key Laboratory of Materials-Oriented Chemical Engineering, School of 
      Pharmaceutical Sciences, Nanjing Tech University, Nanjing, China.
FAU - Lu, Zeping
AU  - Lu Z
AD  - State Key Laboratory of Materials-Oriented Chemical Engineering, School of 
      Pharmaceutical Sciences, Nanjing Tech University, Nanjing, China.
FAU - Nian, Binbin
AU  - Nian B
AD  - State Key Laboratory of Materials-Oriented Chemical Engineering, School of 
      Pharmaceutical Sciences, Nanjing Tech University, Nanjing, China.
FAU - Yang, Shipin
AU  - Yang S
AD  - College of Electrical Engineering and Control Science, Nanjing Tech University, 
      Nanjing, China. Electronic address: spyang@njtech.edu.cn.
FAU - Hu, Yi
AU  - Hu Y
AD  - State Key Laboratory of Materials-Oriented Chemical Engineering, School of 
      Pharmaceutical Sciences, Nanjing Tech University, Nanjing, China. Electronic 
      address: huyi@njtech.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20230914
PL  - England
TA  - J Environ Manage
JT  - Journal of environmental management
JID - 0401664
RN  - 0 (Ionic Liquids)
RN  - 0 (graphene oxide)
RN  - 7782-42-5 (Graphite)
RN  - EC 1.10.3.2 (Laccase)
RN  - 3A3U0GI71G (Magnesium Oxide)
RN  - 0 (Enzymes, Immobilized)
RN  - 0 (Oxides)
RN  - 0 (Phenols)
SB  - IM
MH  - *Ionic Liquids/chemistry
MH  - *Graphite/chemistry
MH  - Laccase/chemistry/metabolism
MH  - Magnesium Oxide
MH  - Molecular Docking Simulation
MH  - Enzymes, Immobilized/chemistry/metabolism
MH  - Oxides
MH  - Phenols
MH  - Magnetic Phenomena
OTO - NOTNLM
OT  - Graphite oxide
OT  - Ionic liquids
OT  - Laccase immobilization
OT  - Molecular dynamic simulations
OT  - Pollutant removal
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/09/17 00:41
MHDA- 2023/10/09 06:41
CRDT- 2023/09/16 18:06
PHST- 2023/06/26 00:00 [received]
PHST- 2023/08/23 00:00 [revised]
PHST- 2023/09/09 00:00 [accepted]
PHST- 2023/10/09 06:41 [medline]
PHST- 2023/09/17 00:41 [pubmed]
PHST- 2023/09/16 18:06 [entrez]
AID - S0301-4797(23)01763-2 [pii]
AID - 10.1016/j.jenvman.2023.118975 [doi]
PST - ppublish
SO  - J Environ Manage. 2023 Nov 15;346:118975. doi: 10.1016/j.jenvman.2023.118975. 
      Epub 2023 Sep 14.