PMID- 37716240
OWN - NLM
STAT- MEDLINE
DCOM- 20231109
LR  - 20231109
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 267
DP  - 2024 Jan 15
TI  - Paper-based microfluidic device for selective detection of peanut allergen Ara h1 
      applying black phosphorus-Au nanocomposites for signal amplification.
PG  - 125188
LID - S0039-9140(23)00939-6 [pii]
LID - 10.1016/j.talanta.2023.125188 [doi]
AB  - This paper developed a portable microfluidic paper-based analysis device (mu-PAD) 
      combined with the electrochemical technique for efficient and sensitive detection 
      of peanut allergen Ara h1. The proposed mu-PAD works based on the variation of 
      differential pulse voltammetry (DPV) response current induced by peanut allergen 
      Ara h1. Black phosphorus (BP)-Au nanocomposites were introduced both to improve 
      the electron transfer rate at the electrode interface for signal amplification, 
      and to immobilize the specific Ara h1 aptamers through Au-S bonding to recognize 
      the target in food matrices. This mu-PAD had good specificity and detection 
      stability for Ara h1 allergen and could complete the entire analysis process 
      within 20 min, achieving a wide linear response range (25-800 ng mL(-1)) and a 
      low detection limit (LOD, 11.8 ng mL(-1)). In the Ara h1 allergen detection 
      applied to real peanut products (cookies, milk, and bread), the constructed mu-PAD 
      obtained acceptable recoveries (93.50%-101.86%) with relative standard deviations 
      (RSDs) of 0.36-2.97% (n = 3), with a good correlation with the ELISA results 
      (R(2) = 0.9956). Therefore, the portable mu-PAD based on BP-Au nanocomposites was 
      demonstrated to provide an effective strategy for rapid analysis and screening of 
      Ara h1 allergen in food, which has broad application prospects.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Pan, Mingfei
AU  - Pan M
AD  - State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science 
      and Technology, Tianjin, 300457, China; Key Laboratory of Food Quality and Health 
      of Tianjin, Tianjin University of Science & Technology, 300457, Tianjin, China.
FAU - Han, Xintong
AU  - Han X
AD  - State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science 
      and Technology, Tianjin, 300457, China; Key Laboratory of Food Quality and Health 
      of Tianjin, Tianjin University of Science & Technology, 300457, Tianjin, China.
FAU - Chen, Sixuan
AU  - Chen S
AD  - State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science 
      and Technology, Tianjin, 300457, China; Key Laboratory of Food Quality and Health 
      of Tianjin, Tianjin University of Science & Technology, 300457, Tianjin, China.
FAU - Yang, Jingying
AU  - Yang J
AD  - State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science 
      and Technology, Tianjin, 300457, China; Key Laboratory of Food Quality and Health 
      of Tianjin, Tianjin University of Science & Technology, 300457, Tianjin, China.
FAU - Wang, Yueyao
AU  - Wang Y
AD  - State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science 
      and Technology, Tianjin, 300457, China; Key Laboratory of Food Quality and Health 
      of Tianjin, Tianjin University of Science & Technology, 300457, Tianjin, China.
FAU - Li, Huilin
AU  - Li H
AD  - State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science 
      and Technology, Tianjin, 300457, China; Key Laboratory of Food Quality and Health 
      of Tianjin, Tianjin University of Science & Technology, 300457, Tianjin, China.
FAU - Wang, Shuo
AU  - Wang S
AD  - State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science 
      and Technology, Tianjin, 300457, China; Key Laboratory of Food Quality and Health 
      of Tianjin, Tianjin University of Science & Technology, 300457, Tianjin, China. 
      Electronic address: s.wang@tust.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20230912
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Allergens)
RN  - 0 (Plant Proteins)
RN  - 0 (Antigens, Plant)
SB  - IM
MH  - *Allergens/analysis
MH  - Plant Proteins/analysis
MH  - Antigens, Plant
MH  - Arachis
MH  - *Nanocomposites
OTO - NOTNLM
OT  - Aptamer
OT  - Black phosphorus nanosheets
OT  - Gold nanoparticles
OT  - Microfluidic paper-based analysis device
OT  - Peanut allergen ara h1
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/09/17 00:41
MHDA- 2023/11/09 06:42
CRDT- 2023/09/16 18:08
PHST- 2023/05/09 00:00 [received]
PHST- 2023/09/04 00:00 [revised]
PHST- 2023/09/08 00:00 [accepted]
PHST- 2023/11/09 06:42 [medline]
PHST- 2023/09/17 00:41 [pubmed]
PHST- 2023/09/16 18:08 [entrez]
AID - S0039-9140(23)00939-6 [pii]
AID - 10.1016/j.talanta.2023.125188 [doi]
PST - ppublish
SO  - Talanta. 2024 Jan 15;267:125188. doi: 10.1016/j.talanta.2023.125188. Epub 2023 
      Sep 12.