PMID- 37716665
OWN - NLM
STAT- MEDLINE
DCOM- 20231127
LR  - 20231127
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 253
IP  - Pt 3
DP  - 2023 Dec 31
TI  - Effects of in vitro fermentation of Atractylodes chinensis (DC.) Koidz. 
      polysaccharide on fecal microbiota and metabolites in patients with type 2 
      diabetes mellitus.
PG  - 126860
LID - S0141-8130(23)03757-1 [pii]
LID - 10.1016/j.ijbiomac.2023.126860 [doi]
AB  - Atractylodes chinensis (DC.) Koidz. polysaccharide (AKP) has been shown to have 
      hypoglycemic activity. In this study, the effects of AKP on fecal microbiota and 
      metabolites in healthy subjects and patients with type 2 diabetes mellitus (T2DM) 
      were investigated using an in vitro simulated digestive fermentation model. AKP 
      were isolated and purified from Atractylodes chinensis (DC.) Koidz. Its main 
      component AKP1 (AKP-0 M, about 78 % of AKP) has an average molecular weight of 
      3.25 kDa with monosaccharide composition of rhamnose, arabinose, and 
      galactosamine in a molar ratio of 1: 1.25: 2.88. Notably, AKP fermentation might 
      improve the intestinal microbiota of T2DM patients by the enrichment of some 
      specific bacteria rather than the increase of microbial diversity. The addition 
      of AKP specifically enriched Bifidobacteriaceae and weakened the proportion of 
      Escherichia-Shigella. Moreover, AKP also increased the levels of short-chain 
      fatty acids without affecting total gut gas production, suggesting that AKP could 
      have beneficial effects while avoiding flatulence. Metabolomic analysis revealed 
      that ARP fermentation caused changes in some metabolites, which were mainly 
      related to energy metabolism and amino acid metabolism. Importantly, ARP 
      fermentation significantly increased the level of myo-inositol, an insulin 
      sensitizer. In addition, a significant correlation was observed between specific 
      microbiota and differential metabolites. This study has laid a theoretical 
      foundation for AKP application in functional foods.
CI  - Copyright (c) 2023. Published by Elsevier B.V.
FAU - Zhang, Xin
AU  - Zhang X
AD  - College of Biotechnology, Tianjin University of Science and Technology, Tianjin 
      300457, China; Key Laboratory of Industrial Fermentation Microbiology, Ministry 
      of Education and Tianjin, Tianjin 300457, China.
FAU - Ma, Qian
AU  - Ma Q
AD  - College of Biotechnology, Tianjin University of Science and Technology, Tianjin 
      300457, China; Key Laboratory of Industrial Fermentation Microbiology, Ministry 
      of Education and Tianjin, Tianjin 300457, China.
FAU - Jia, Lina
AU  - Jia L
AD  - College of Biotechnology, Tianjin University of Science and Technology, Tianjin 
      300457, China; Key Laboratory of Industrial Fermentation Microbiology, Ministry 
      of Education and Tianjin, Tianjin 300457, China.
FAU - He, Hongpeng
AU  - He H
AD  - College of Biotechnology, Tianjin University of Science and Technology, Tianjin 
      300457, China; Key Laboratory of Industrial Fermentation Microbiology, Ministry 
      of Education and Tianjin, Tianjin 300457, China.
FAU - Zhang, Tongcun
AU  - Zhang T
AD  - College of Biotechnology, Tianjin University of Science and Technology, Tianjin 
      300457, China; Key Laboratory of Industrial Fermentation Microbiology, Ministry 
      of Education and Tianjin, Tianjin 300457, China.
FAU - Jia, Weiguo
AU  - Jia W
AD  - The Center of Gerontology and Geriatrics, National Clinical Research Center of 
      Geriatrics, West China Hospital, Sichuan University, Chengdu 610041, China.
FAU - Zhu, Liying
AU  - Zhu L
AD  - Institute of Food Science Zhejiang Academy of Agricultural Sciences, Hangzhou 
      310021, China.
FAU - Qi, Wei
AU  - Qi W
AD  - College of Biotechnology, Tianjin University of Science and Technology, Tianjin 
      300457, China; Key Laboratory of Industrial Fermentation Microbiology, Ministry 
      of Education and Tianjin, Tianjin 300457, China. Electronic address: 
      qiweismiling@163.com.
FAU - Wang, Nan
AU  - Wang N
AD  - College of Biotechnology, Tianjin University of Science and Technology, Tianjin 
      300457, China; Key Laboratory of Industrial Fermentation Microbiology, Ministry 
      of Education and Tianjin, Tianjin 300457, China. Electronic address: 
      wn929@tust.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20230914
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Humans
MH  - *Atractylodes/chemistry
MH  - Fermentation
MH  - *Diabetes Mellitus, Type 2/drug therapy
MH  - *Microbiota
MH  - Polysaccharides/chemistry
OTO - NOTNLM
OT  - Atractylodes chinensis (DC.) Koidz.
OT  - Fecal microbiota
OT  - Metabolites
OT  - Type 2 diabetes mellitus
COIS- Declaration of competing interest Authors declare that no competing financial 
      interests could influence the work reported in this paper.
EDAT- 2023/09/17 00:42
MHDA- 2023/11/27 12:42
CRDT- 2023/09/16 19:18
PHST- 2023/06/20 00:00 [received]
PHST- 2023/09/01 00:00 [revised]
PHST- 2023/09/09 00:00 [accepted]
PHST- 2023/11/27 12:42 [medline]
PHST- 2023/09/17 00:42 [pubmed]
PHST- 2023/09/16 19:18 [entrez]
AID - S0141-8130(23)03757-1 [pii]
AID - 10.1016/j.ijbiomac.2023.126860 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2023 Dec 31;253(Pt 3):126860. doi: 
      10.1016/j.ijbiomac.2023.126860. Epub 2023 Sep 14.