PMID- 37728841
OWN - NLM
STAT- Publisher
LR  - 20230920
IS  - 1559-0305 (Electronic)
IS  - 1073-6085 (Linking)
DP  - 2023 Sep 20
TI  - CircPRRC2C Promotes the Oncogenic Phenotypes of Laryngeal Squamous Cell Carcinoma 
      Cells via MiR-136-5p/HOXD11 Axis.
LID - 10.1007/s12033-023-00868-y [doi]
AB  - The involvement of circular RNAs (circRNAs) in laryngeal squamous cell carcinoma 
      (LSCC) carcinogenesis has gradually been proposed. Herein, we aimed to explore 
      the function and mechanism of circPRRC2C in LSCC. Quantitative real-time 
      polymerase chain reaction (qRT-PCR) and Western blotting were used for detecting 
      the content of genes and proteins. In vitro experiments were conducted using 
      5-ethynyl-2'-deoxyuridine, colony formation, flow cytometry, and transwell 
      assays. The binding between miR-136-5p and circPRRC2C or Homeobox D11 (HOXD11) 
      was confirmed by using the dual-luciferase reporter assay. The murine xenograft 
      model was established for in vivo analysis. The commercial kit was used for 
      exosome separation. CircPRRC2C is a stable circRNA, and was highly expressed in 
      LSCC tissues and cell lines. Functionally, circPRRC2C deficiency impaired LSCC 
      cell proliferation, migration and invasion but induced cell apoptosis in vitro 
      and impeded tumor growth in vivo, however, circPRRC2C overexpression showed the 
      exact opposite effects. Mechanistically, circPRRC2C directly targeted miR-136-5p, 
      which showed inhibitory effects on the growth and mobility of LSCC cells. 
      Meanwhile, miR-136-5p directly targeted HOXD11, and circPRRC2C/miR-136-5p/HOXD11 
      formed a feedback loop in LSCC cells. Further rescue assays exhibited that 
      circPRRC2C exerted its effects by miR-136-5p/HOXD11 axis. In addition, circPRRC2C 
      was stably packaged into exosomes and showed potential diagnostic value for 
      LSCC. CircPRRC2C acted as an oncogene to promote LSCC cell oncogenic phenotypes 
      via miR-136-5p/HOXD11 axis, besides, circPRRC2C was stably packaged into 
      exosomes, indicating the potential application of circPRRC2C-targeting agents in 
      the treatment in LSCC.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Peng, Xiu
AU  - Peng X
AD  - Department of Otolaryngology, Wuhan Hospital of Traditional Chinese Medicine, 
      No.49, Nihuangpi Road, Wuhan, 430022, Hubei, China.
FAU - Chen, Xintian
AU  - Chen X
AD  - College of Life Science, South-Central MinZu University, Wuhan, 430074, China.
AD  - College of Resource and Environmental Science, South-Central MinZu University, 
      Wuhan, 430074, China.
FAU - Peng, Shuai
AU  - Peng S
AD  - Hubei Provincial Engineering Laboratory for Clean Production and High Value 
      Utilization of Bio-Based Textile Materials, Wuhan Textile University, Wuhan, 
      430073, China. gwzps211@126.com.
AD  - College of Life Science, South-Central MinZu University, Wuhan, 430074, China. 
      gwzps211@126.com.
FAU - Chen, Yingming
AU  - Chen Y
AD  - College of Resource and Environmental Science, South-Central MinZu University, 
      Wuhan, 430074, China.
FAU - Li, Yong
AU  - Li Y
AUID- ORCID: 0009-0003-9787-6139
AD  - Department of Otolaryngology, Wuhan Hospital of Traditional Chinese Medicine, 
      No.49, Nihuangpi Road, Wuhan, 430022, Hubei, China. niyoo19862022@163.com.
FAU - Tian, Yang
AU  - Tian Y
AD  - College of Life Science and Technology, Huazhong University of Science and 
      Technology, Wuhan, 430074, China.
LA  - eng
PT  - Journal Article
DEP - 20230920
PL  - Switzerland
TA  - Mol Biotechnol
JT  - Molecular biotechnology
JID - 9423533
SB  - IM
OTO - NOTNLM
OT  - Apoptosis
OT  - CircPRRC2C
OT  - Exosome
OT  - HOXD11
OT  - MiR-136-5p
EDAT- 2023/09/20 12:51
MHDA- 2023/09/20 12:51
CRDT- 2023/09/20 11:31
PHST- 2023/03/14 00:00 [received]
PHST- 2023/08/21 00:00 [accepted]
PHST- 2023/09/20 12:51 [medline]
PHST- 2023/09/20 12:51 [pubmed]
PHST- 2023/09/20 11:31 [entrez]
AID - 10.1007/s12033-023-00868-y [pii]
AID - 10.1007/s12033-023-00868-y [doi]
PST - aheadofprint
SO  - Mol Biotechnol. 2023 Sep 20. doi: 10.1007/s12033-023-00868-y.