PMID- 37732576
OWN - NLM
STAT- MEDLINE
DCOM- 20230922
LR  - 20230922
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 39
IP  - 9
DP  - 2023 Sep
TI  - [Nano-vesicles derived from M1 macrophages affect the development of mouse 
      endometriosis by polarizing M2 macrophages to M1 phenotype].
PG  - 807-815
AB  - Objective To explore whether nano-vesicles derived from M1 macrophages (M1-NVs) 
      can reprogram M2 macrophages into M1 phenotype and further affect the development 
      of endometriosis (EMS). Methods Extracellular vesicles (EVs) were isolated from 
      macrophage culture supernatant by differential centrifugation. Immunofluorescence 
      cytochemistry was used to detect the expression of vimentin, CD31 and F4/80 to 
      identify endometrial stromal cells (EMS-ESCs), HUVECs and polarized peritoneal 
      macrophages of EMS patients. M1-NVs were prepared by filtering cell suspension 
      through (5, 1, 0.4, 0.22)mum polycarbonate membrane filters after syringe 
      aspiration at 0-4 DegreesCelsius. Flow cytometry was used to analyze the 
      polarization of RAW264.7 mouse peritoneal macrophages in vitro, and reverse 
      transcription PCR (RT-qPCR) was employed to detect mRNA expression of VEGF, CD86, 
      interleukin-6 (IL-6), IL-1beta, tumor necrosis factor alpha (TNF-alpha), arginase 1 (Arg1), 
      CD163, CD206, and IL-10. PKH67-labeled M1-NVs were co-cultured with EMS-ESCs, 
      HUVECs and macrophages. And tubule formation experiments were conducted to assess 
      the impact of M1-NVs on the tubule formation of HUVECs. Transwell(TM) invasion 
      and migration assays were employed to evaluate changes in the migration and 
      invasion abilities of EMS-ESCs. Results By monitoring the contents of NVs, it was 
      found that NVs contained much more protein and other bioactive particles than the 
      same amount of EVs; immunofluorescence staining results showed that PKH67 labeled 
      M1-NVs were internalized by EMS-ESCs, HUVECs and macrophages when co-cultured. 
      The results of flow cytometry and RT-qPCR multi-target analysis showed that after 
      treatment with different concentrations of M1-NVs or M0-NVs, 20 mug/mL of M1-NVs 
      could effectively reprogram M2 macrophages into M1 macrophages compared with 
      M0-NVs. Transewell(TM) results showed that compared with the blank group and 
      M0-NVs group, the number of EMS-ESCs migrating from the upper chamber to the 
      lower chamber after M1-NV treatment was significantly reduced, while the number 
      of EMS-ESCs treated with M2NVs increased significantly. The invasion situation 
      was similar to the migration situation, indicating that M1-NVs directly or 
      indirectly inhibited invasion, migration and tubule formation of EMS-ESCs in 
      vitro. Conclusion M1-NVs reprogrammes M2 macrophages into M1 macrophages by 
      internalization of primary cells and macrophages, thereby inhibiting invasion, 
      migration and angiogenesis of EMS-ESCs, and further hindering the occurrence and 
      development of EMS.
FAU - Meng, Qiu
AU  - Meng Q
AD  - Department of Obstetrics and Gynecology, Haikou Hospital of Central South 
      University Xiangya School of Medicine, Haikou 570208, China.
FAU - Xie, Xingrun
AU  - Xie X
AD  - Department of Radiology, Haikou Hospital of Central South University Xiangya 
      School of Medicine, Haikou 570208, China.
FAU - Huang, Shouguo
AU  - Huang S
AD  - Department of Obstetrics and Gynecology, Haikou Hospital of Central South 
      University Xiangya School of Medicine, Haikou 570208, China. *Corresponding 
      author, E-mail: shouguohuang@126.com.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (PKH67)
SB  - IM
MH  - Female
MH  - Humans
MH  - Animals
MH  - Mice
MH  - *Endometriosis
MH  - Macrophages
MH  - Macrophages, Peritoneal
MH  - Coculture Techniques
EDAT- 2023/09/21 12:43
MHDA- 2023/09/22 06:42
CRDT- 2023/09/21 07:33
PHST- 2023/09/22 06:42 [medline]
PHST- 2023/09/21 12:43 [pubmed]
PHST- 2023/09/21 07:33 [entrez]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2023 Sep;39(9):807-815.