PMID- 37732790
OWN - NLM
STAT- Publisher
LR  - 20231020
IS  - 2165-0497 (Electronic)
IS  - 2165-0497 (Linking)
VI  - 11
IP  - 5
DP  - 2023 Sep 21
TI  - Lugdunin production and activity in Staphylococcus lugdunensis isolates are 
      associated with its genotypes.
PG  - e0129823
LID - 10.1128/spectrum.01298-23 [doi]
LID - e01298-23
AB  - Lugdunin produced by Staphylococcus lugdunensis has been shown to have broad 
      inhibitory activity against Gram-positive bacteria; however, lugdunin activity 
      among S. lugdunensis isolates and its association with different agr, SCCmec, and 
      sequence types remain unclear. We used matrix-assisted laser desorption 
      ionization-time-of-flight mass spectrometry to identify S. lugdunensis and 
      collected 202 S. lugdunensis samples for further assays. Agar spot tests were 
      performed to characterize S. lugdunensis lugdunin production and activity. 
      Multilocus sequence typing, SCCmec, and agr genotyping were performed on S. 
      lugdunensis. In all, 91 Staphylococcus aureus strains with varying vancomycin 
      susceptibilities were used to examine lugdunin activity in S. lugdunensis. In 
      total, 48 S. lugdunensis strains (23.8%) were found to be oxacillin-resistant S. 
      lugdunensis (ORSL), whereas 154 (76.2%) were classified as oxacillin-sensitive S. 
      lugdunensis (OSSL). Moreover, 16 (33.3%) ORSL and 35 (22.7%) OSSL strains showed 
      antibacterial activity against S. aureus. Our data showed that most 
      lugdunin-producing ORSL strains (14/48, 29.2%) were of ST3-SCCmec V-agr II 
      genotypes, whereas most lugdunin-producing OSSL strains (15/154, 9.7%) were of 
      ST3-agr II, followed by ST1-agr I (10/154, 6.5%). Our data also revealed that 
      lugdunin exhibited weak inhibitory activity against the VISA ST239 isolate. In 
      addition, we observed that ST239 VSSA was more resistant to lugdunin than ST5, 
      ST59, and ST45 VSSA. Taken together, our data pioneered the epidemiology of 
      lugdunin production in S. lugdunensis isolates and revealed its association with 
      genotypes. However, further molecular and bioinformatics investigations are 
      needed to elucidate the regulatory mechanisms of lugdunin production and 
      activity. IMPORTANCE Lugdunin is active against both methicillin-resistant 
      Staphylococcus aureus and vancomycin-resistant enterococci by dissipating their 
      membrane potential. However, the association of lugdunin activity with the 
      genotypes of Staphylococcus lugdunensis has not been addressed. Here, we show the 
      high prevalence of lugdunin-producing strains among ST1 (83.3%), ST2 (66.7%), and 
      ST3 (53.3%) S. lugdunensis. Moreover, we identified the antibacterial activity of 
      lugdunin-producing strains against VISA and hVISA. These results shed light on 
      the potential application of lugdunin for the treatment of drug-resistant 
      pathogens.
FAU - Chang, Shih-Cheng
AU  - Chang SC
AUID- ORCID: 0000-0001-5921-7974
AD  - Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital , Taoyuan, 
      Taiwan.
AD  - Department of Medical Biotechnology and Laboratory Science, College of Medicine, 
      Chang Gung University , Taoyuan, Taiwan.
FAU - Kao, Cheng-Yen
AU  - Kao CY
AD  - Institute of Microbiology and Immunology, College of Life Sciences, National Yang 
      Ming Chiao Tung University , Taipei, Taiwan.
FAU - Lin, Lee-Chung
AU  - Lin LC
AD  - Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital , Taoyuan, 
      Taiwan.
FAU - Hidrosollo, Jazon Harl
AU  - Hidrosollo JH
AD  - Institute of Microbiology and Immunology, College of Life Sciences, National Yang 
      Ming Chiao Tung University , Taipei, Taiwan.
FAU - Lu, Jang-Jih
AU  - Lu JJ
AD  - Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital , Taoyuan, 
      Taiwan.
AD  - Department of Medical Biotechnology and Laboratory Science, College of Medicine, 
      Chang Gung University , Taoyuan, Taiwan.
AD  - Department of Medicine, College of Medicine, Chang Gung University , Taoyuan, 
      Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20230921
PL  - United States
TA  - Microbiol Spectr
JT  - Microbiology spectrum
JID - 101634614
SB  - IM
PMC - PMC10580833
OTO - NOTNLM
OT  - S. aureus
OT  - Staphylococcus lugdunensis
OT  - genotype
OT  - lugdunin
COIS- The authors declare no conflict of interest.
EDAT- 2023/09/21 12:42
MHDA- 2023/09/21 12:42
PMCR- 2023/09/21
CRDT- 2023/09/21 09:13
PHST- 2023/09/21 12:42 [medline]
PHST- 2023/09/21 12:42 [pubmed]
PHST- 2023/09/21 09:13 [entrez]
PHST- 2023/09/21 00:00 [pmc-release]
AID - 01298-23 [pii]
AID - spectrum.01298-23 [pii]
AID - 10.1128/spectrum.01298-23 [doi]
PST - aheadofprint
SO  - Microbiol Spectr. 2023 Sep 21;11(5):e0129823. doi: 10.1128/spectrum.01298-23.