PMID- 37733709
OWN - NLM
STAT- MEDLINE
DCOM- 20230925
LR  - 20230926
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 18
IP  - 9
DP  - 2023
TI  - Dexamethasone treatment of murine auditory hair cells and cochlear explants 
      attenuates tumor necrosis factor-alpha-initiated apoptotic damage.
PG  - e0291780
LID - 10.1371/journal.pone.0291780 [doi]
LID - e0291780
AB  - The most common cause of sensorineural hearing loss is damage of auditory hair 
      cells. Tumor necrosis factor-alpha (TNF-alpha) is closely associated with 
      sensorineural hearing loss. The present study examined the preconditioning effect 
      of dexamethasone (DEX) on TNF-alpha-induced ototoxicity in mouse auditory hair cells 
      (HEI-OC1) and cochlear explants. Treatment of HEI-OC1 with 10 ng/ml TNF-alpha for 24 
      h decreased cell viability, increased the accumulation of reactive oxygen species 
      (ROS), and induced caspase-mediated apoptotic signaling pathways. Pretreatment 
      with 10 nM DEX for 6 h before TNF-alpha exposure restored cell viability, decreased 
      ROS accumulation, and attenuated apoptotic signaling activation induced by TNF-alpha. 
      Incubation of cochlear explants with 20 ng/ml TNF-alpha for 24 h resulted in 
      significant loss of both inner hair cells (IHCs) and outer hair cells (OHCs) and 
      an increase in apoptotic activation accessed by annexin V staining. The cochlear 
      explants pre-incubated with 10 nM DEX attenuated TNF-alpha ototoxicity in both IHCs 
      and OHCs and apoptotic cell death. These results indicated that DEX plays a 
      protective role in ototoxicity induced by TNF-alpha through attenuation of 
      caspase-dependent apoptosis signaling pathway and ROS accumulation.
CI  - Copyright: (c) 2023 Kang et al. This is an open access article distributed under 
      the terms of the Creative Commons Attribution License, which permits unrestricted 
      use, distribution, and reproduction in any medium, provided the original author 
      and source are credited.
FAU - Kang, Byung Chul
AU  - Kang BC
AUID- ORCID: 0000-0002-5536-7796
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Ulsan University 
      Hospital, University of Ulsan College of Medicine, Ulsan, Korea.
FAU - Yi, Junyeong
AU  - Yi J
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Asan Medical Center, 
      University of Ulsan College of Medicine, Seoul, Korea.
FAU - Kim, Song Hee
AU  - Kim SH
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Ulsan University 
      Hospital, University of Ulsan College of Medicine, Ulsan, Korea.
FAU - Pak, Jhang Ho
AU  - Pak JH
AUID- ORCID: 0000-0001-5104-5995
AD  - Department of Convergence Medicine, University of Ulsan College of Medicine and 
      Asan Institute for Life Sciences, Asan Medical Center, Seoul, Korea.
FAU - Chung, Jong Woo
AU  - Chung JW
AUID- ORCID: 0000-0003-0765-9134
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Asan Medical Center, 
      University of Ulsan College of Medicine, Seoul, Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230921
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Reactive Oxygen Species)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Mice
MH  - Tumor Necrosis Factor-alpha
MH  - *Ototoxicity
MH  - Reactive Oxygen Species
MH  - Hair Cells, Auditory, Outer
MH  - *Hearing Loss, Sensorineural
MH  - Dexamethasone/pharmacology
PMC - PMC10513268
COIS- The authors have declared that no competing interests exist.
EDAT- 2023/09/21 18:42
MHDA- 2023/09/25 06:42
PMCR- 2023/09/21
CRDT- 2023/09/21 13:43
PHST- 2023/06/19 00:00 [received]
PHST- 2023/09/05 00:00 [accepted]
PHST- 2023/09/25 06:42 [medline]
PHST- 2023/09/21 18:42 [pubmed]
PHST- 2023/09/21 13:43 [entrez]
PHST- 2023/09/21 00:00 [pmc-release]
AID - PONE-D-23-19102 [pii]
AID - 10.1371/journal.pone.0291780 [doi]
PST - epublish
SO  - PLoS One. 2023 Sep 21;18(9):e0291780. doi: 10.1371/journal.pone.0291780. 
      eCollection 2023.